LOCUS       Exported     3272 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3272
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1285..1332
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3160..3176
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3177..3208
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /label="MMSYN1_0606"
                     /note="pgk: phosphoglycerate kinase"
                     /translation="MNYNNKKTLKDIDVKNKTVLVRVDFNVPIQSGVITDDNRIIAALPTINYLLENDAKIVLFSHLSRIKSKEDKLKKSLAPVAKRLEELLNKQVKFINQTRGQELEQAISSLQSKEIILVENTRFEDVLDDQVVKYESKNNYELGKYWASLGEVFVNDAFGTAHRAHASNVGIASNIKVSAIGFLVEKELKMLSQAVNEPKKPFIAILGGAKVSDKIGVIENLLPKVDKLLIGGGMSYTFLKALGKTIGKSLLEEDKIDLAKHYLEIGKDKIVVPVDTACAKEFADVSPTIFEGNIPDEWDGLDAGPKTIELFKQEIKKAKTIVWNGPVGVFEFKNFENGTKSVCQAIAEQTQNGAFTLIGGGDSASAAINMGFKDKFSWISTGGGASLEFMEGKELLGISAIQDK"
     primer_bind     complement(1337..1353)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1420..2008)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2179..3039)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3040..3144)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /translation="MNYNNKKTLKDIDVKNKTVLVRVDFNVPIQSGVITDDNRIIAALPTINYLLENDAKIVLFSHLSRIKSKEDKLKKSLAPVAKRLEELLNKQVKFINQTRGQELEQAISSLQSKEIILVENTRFEDVLDDQVVKYESKNNYELGKYWASLGEVFVNDAFGTAHRAHASNVGIASNIKVSAIGFLVEKELKMLSQAVNEPKKPFIAILGGAKVSDKIGVIENLLPKVDKLLIGGGMSYTFLKALGKTIGKSLLEEDKIDLAKHYLEIGKDKIVVPVDTACAKEFADVSPTIFEGNIPDEWDGLDAGPKTIELFKQEIKKAKTIVWNGPVGVFEFKNFENGTKSVCQAIAEQTQNGAFTLIGGGDSASAAINMGFKDKFSWISTGGGASLEFMEGKELLGISAIQDK"
                     /label="MMSYN1_0606"
                     /note="pgk: phosphoglycerate kinase"
ORIGIN
        1 ATGAACTATA ACAATAAGAA AACTTTAAAG GACATCGATG TGAAAAATAA AACTGTCCTT
       61 GTCCGTGTCG ATTTCAACGT ACCAATCCAG AGTGGCGTTA TCACGGATGA CAATCGCATT
      121 ATCGCAGCTT TGCCGACAAT CAACTATCTG CTTGAGAACG ATGCAAAAAT CGTATTGTTT
      181 TCCCACCTTT CGCGCATTAA ATCGAAGGAG GATAAACTGA AGAAATCCCT TGCTCCGGTA
      241 GCAAAGCGTT TGGAGGAGCT TCTGAACAAA CAGGTAAAAT TCATCAACCA AACTCGTGGG
      301 CAGGAGTTAG AACAAGCAAT CTCATCGTTG CAATCCAAGG AGATCATTTT GGTGGAGAAC
      361 ACGCGCTTTG AGGATGTCCT GGATGATCAA GTGGTGAAAT ATGAATCGAA AAATAATTAC
      421 GAATTAGGGA AATATTGGGC GAGTCTGGGA GAAGTATTCG TTAACGATGC CTTTGGGACG
      481 GCCCACCGCG CCCACGCCAG TAACGTTGGA ATTGCGTCGA ACATTAAAGT TAGTGCAATC
      541 GGTTTTCTGG TCGAAAAAGA ACTGAAGATG TTGTCTCAAG CAGTTAATGA ACCTAAGAAG
      601 CCCTTCATTG CAATTTTAGG AGGAGCGAAA GTCTCTGACA AAATTGGCGT GATCGAAAAT
      661 CTGTTGCCTA AAGTAGATAA ATTGCTTATT GGGGGCGGGA TGTCTTATAC GTTTCTGAAG
      721 GCCCTTGGCA AGACTATCGG GAAGTCATTA CTTGAAGAAG ATAAGATTGA TCTGGCTAAA
      781 CATTACTTGG AAATTGGAAA GGATAAAATC GTCGTCCCAG TGGATACGGC ATGTGCCAAG
      841 GAGTTTGCGG ACGTGAGTCC AACAATCTTT GAGGGCAATA TTCCAGATGA ATGGGATGGG
      901 CTGGACGCAG GGCCAAAAAC AATTGAGTTG TTCAAGCAAG AAATCAAAAA GGCCAAGACA
      961 ATCGTATGGA ATGGGCCAGT GGGAGTTTTC GAATTTAAGA ATTTCGAGAA TGGTACCAAG
     1021 TCTGTTTGCC AGGCAATTGC AGAGCAGACG CAAAACGGAG CCTTTACCTT AATCGGCGGG
     1081 GGCGATAGTG CGTCAGCAGC TATTAACATG GGATTTAAGG ACAAATTTTC TTGGATTTCA
     1141 ACCGGAGGCG GCGCTAGCTT AGAGTTCATG GAAGGGAAAG AACTTTTAGG GATCTCCGCT
     1201 ATCCAGGATA AGTGAAGAGC TTAGAGACCG GTACTAGTTC CTGCAGGGCT CGAGGTGTCA
     1261 ATCGTCGGAG CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG
     1321 AGGGGTTTTT TGCATGGTCA TAGCTGTTTC CTGAGAGCTT GGCAGGTGAT GACACACATT
     1381 AACAAATTTC GTGAGGAGTC TCCAGAAGAA TGCCATTAAT TTCCATAGGC TCCGCCCCCC
     1441 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA
     1501 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC
     1561 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC
     1621 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA
     1681 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC
     1741 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG
     1801 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG
     1861 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG
     1921 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA
     1981 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA
     2041 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT
     2101 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA
     2161 GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG
     2221 TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA
     2281 GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA GAACCACGCT CACCGGCTCC
     2341 AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC
     2401 TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC
     2461 AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC
     2521 GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC
     2581 CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT
     2641 GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC
     2701 ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG
     2761 TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG
     2821 CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT
     2881 CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC
     2941 ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA
     3001 AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA
     3061 TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA
     3121 AAATAAACAA ATAGGGGTTC CGCGCACCTG CACCAGTCAG TAAAACGACG GCCAGTAGTC
     3181 AAAAGCCTCC GACCGGAGGC TTTTGACTTG GTCAGGTGGA GTGAATTCGC GGCCGCTTCT
     3241 AGAGGCTCTT CGTAATTACG AAGAGGTCTC TA
//