LOCUS       Exported     3074 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3074
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1087..1134
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2962..2978
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2979..3010
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
                     /label="tonB terminator"
     CDS             1..0
                     /note=""
                     /translation="MSKKVAINGFGRIGRLTFRQLFEKDIEIVAINDLTDTKTLAYLLEFDTAQGIFCEGEISHTDNSIIVKGKEVKVFAEKDASNLPWSELKVDLVIESTGFYTDKEKASAHIKAGAKKVVISAPATGDLKTVVYGVNHKSLTSDDVIISGASCTTNCLTPFTKALDDAFTIKKGFMTTVHAVTNDQRLLDLNHKDVRRGRAAAWNIVPSTTGAAKAVSLVLPHLKGKLDGYALRVPTITGSITDLTVEFENQELTVEQINNAVKKALEADADLAKAMKYETRPIVSSDIIGSKYGSIFDATLTKVMNVDGKQLVKVCSWYDNESSYVSQLVRTTIYFMSL"
                     /label="MMSYN1_0607"
     primer_bind     complement(1139..1155)
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 rev"
     rep_origin      complement(1222..1810)
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /label="ori"
     CDS             complement(1981..2841)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2842..2946)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /label="MMSYN1_0607"
                     /note=""
                     /translation="MSKKVAINGFGRIGRLTFRQLFEKDIEIVAINDLTDTKTLAYLLEFDTAQGIFCEGEISHTDNSIIVKGKEVKVFAEKDASNLPWSELKVDLVIESTGFYTDKEKASAHIKAGAKKVVISAPATGDLKTVVYGVNHKSLTSDDVIISGASCTTNCLTPFTKALDDAFTIKKGFMTTVHAVTNDQRLLDLNHKDVRRGRAAAWNIVPSTTGAAKAVSLVLPHLKGKLDGYALRVPTITGSITDLTVEFENQELTVEQINNAVKKALEADADLAKAMKYETRPIVSSDIIGSKYGSIFDATLTKVMNVDGKQLVKVCSWYDNESSYVSQLVRTTIYFMSL"
ORIGIN
        1 ATGTCAAAGA AAGTCGCCAT TAATGGATTT GGACGTATTG GTCGCCTTAC TTTCCGCCAA
       61 TTGTTCGAGA AAGACATCGA GATTGTCGCA ATTAACGATT TGACTGACAC TAAGACTCTT
      121 GCGTATTTGT TGGAGTTTGA TACCGCACAA GGTATCTTTT GTGAAGGTGA GATTTCACAC
      181 ACAGACAACA GCATCATCGT CAAGGGGAAG GAAGTAAAAG TATTCGCCGA AAAAGACGCC
      241 AGTAATTTAC CCTGGTCTGA GTTAAAAGTT GATCTTGTTA TCGAAAGCAC AGGATTCTAC
      301 ACAGATAAAG AAAAAGCCAG TGCACACATC AAAGCCGGAG CGAAAAAAGT CGTCATCAGC
      361 GCTCCTGCAA CTGGAGACTT AAAAACGGTC GTCTACGGAG TGAATCATAA ATCATTGACA
      421 TCTGATGATG TCATTATCTC GGGTGCATCT TGTACGACAA ATTGTTTAAC ACCTTTCACG
      481 AAAGCCCTGG ATGATGCCTT CACGATTAAG AAGGGCTTCA TGACCACTGT ACACGCAGTA
      541 ACGAATGACC AACGCCTGCT TGACCTGAAC CATAAAGACG TTCGTCGTGG CCGCGCCGCT
      601 GCATGGAACA TCGTACCTTC AACCACTGGT GCAGCTAAGG CAGTAAGCCT TGTCTTACCG
      661 CATCTTAAAG GCAAACTTGA TGGATATGCC TTGCGTGTCC CAACGATCAC AGGATCTATC
      721 ACAGACTTAA CAGTCGAATT TGAAAACCAA GAGTTAACAG TGGAACAAAT CAATAACGCT
      781 GTGAAGAAAG CGCTTGAAGC GGATGCCGAT CTGGCGAAGG CAATGAAGTA TGAAACTCGC
      841 CCCATTGTTT CATCAGATAT CATTGGCAGT AAATACGGTT CCATTTTCGA CGCCACCTTA
      901 ACCAAGGTGA TGAACGTCGA CGGCAAGCAA CTGGTGAAAG TTTGCTCGTG GTACGACAAT
      961 GAATCTAGCT ACGTGTCGCA ACTTGTTCGC ACAACCATTT ATTTCATGAG CCTGTGAAGA
     1021 GCTTAGAGAC CGGTACTAGT TCCTGCAGGG CTCGAGGTGT CAATCGTCGG AGCCGCTGAG
     1081 CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCATGGT
     1141 CATAGCTGTT TCCTGAGAGC TTGGCAGGTG ATGACACACA TTAACAAATT TCGTGAGGAG
     1201 TCTCCAGAAG AATGCCATTA ATTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA
     1261 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC
     1321 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC
     1381 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG
     1441 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA
     1501 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC
     1561 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC
     1621 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG
     1681 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA
     1741 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA
     1801 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA
     1861 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT
     1921 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG
     1981 TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT
     2041 AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC
     2101 CAGTGCTGCA ATGATACCGC GAGAACCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA
     2161 CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA
     2221 GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA
     2281 CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT
     2341 CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC
     2401 GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT
     2461 CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC
     2521 TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG
     2581 CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT
     2641 CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC
     2701 CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG
     2761 CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC
     2821 ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG
     2881 TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT
     2941 TCCGCGCACC TGCACCAGTC AGTAAAACGA CGGCCAGTAG TCAAAAGCCT CCGACCGGAG
     3001 GCTTTTGACT TGGTCAGGTG GAGTGAATTC GCGGCCGCTT CTAGAGGCTC TTCGTAATTA
     3061 CGAAGAGGTC TCTA
//