LOCUS       Exported     2996 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2996
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1009..1056
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2884..2900
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2901..2932
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
                     /label="tonB terminator"
     CDS             1..0
                     /label="MMSYN1_0608"
                     /note="DnaI: Primosomal protein"
                     /translation="MKLSDYKNNVKIKKLIEDSQNSNDIITDKVLLENQNILDEFLLNYKECNLDTKCEQVVKNYQVDLVFKDHQFYLKNVLCVHGKQTEKLFIIKKNYWFCDFDLNLFHLTIDEYFNTQLNNATFTLLDQNEKNIRKTILKTIIKQIQKGYKKGFYLYGNSGVGKTYIFKVLANTLASKNKTVIFSTLRSLIDKLKESFNSSEINSLTLIKKIKTVDFLFLDDIGGENLSLWARDDFLFEVLNYRMENQKPTFFTSNFSIDLLEKNLQFTKQYNIFLTTQDVFKLEKIKIDRLISRIKTLAKEINLTGKNKRQTN"
     primer_bind     complement(1061..1077)
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 rev"
     rep_origin      complement(1144..1732)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1903..2763)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2764..2868)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /translation="MKLSDYKNNVKIKKLIEDSQNSNDIITDKVLLENQNILDEFLLNYKECNLDTKCEQVVKNYQVDLVFKDHQFYLKNVLCVHGKQTEKLFIIKKNYWFCDFDLNLFHLTIDEYFNTQLNNATFTLLDQNEKNIRKTILKTIIKQIQKGYKKGFYLYGNSGVGKTYIFKVLANTLASKNKTVIFSTLRSLIDKLKESFNSSEINSLTLIKKIKTVDFLFLDDIGGENLSLWARDDFLFEVLNYRMENQKPTFFTSNFSIDLLEKNLQFTKQYNIFLTTQDVFKLEKIKIDRLISRIKTLAKEINLTGKNKRQTN"
                     /label="MMSYN1_0608"
                     /note="DnaI: Primosomal protein"
ORIGIN
        1 ATGAAACTGT CTGACTACAA GAATAACGTC AAGATCAAAA AACTGATTGA GGACTCTCAA
       61 AACTCTAATG ACATCATTAC TGATAAGGTG CTGCTGGAAA ACCAAAATAT TTTAGATGAG
      121 TTTCTGCTTA ACTACAAGGA ATGTAATTTA GACACAAAAT GTGAGCAAGT TGTGAAGAAC
      181 TATCAGGTAG ATTTGGTCTT TAAGGATCAC CAATTCTATC TTAAAAATGT GTTATGCGTG
      241 CATGGGAAGC AGACCGAAAA GTTGTTTATT ATTAAAAAGA ACTATTGGTT TTGCGACTTT
      301 GATCTGAACC TGTTCCATCT TACGATTGAC GAATATTTTA ATACACAGCT TAACAACGCC
      361 ACTTTCACTC TGTTGGATCA AAATGAAAAG AATATCCGCA AGACTATTCT TAAGACTATC
      421 ATTAAGCAAA TCCAAAAGGG CTACAAAAAA GGGTTCTATT TATACGGAAA TAGCGGTGTG
      481 GGTAAGACCT ATATTTTCAA GGTCCTTGCC AATACCCTTG CCAGCAAAAA CAAAACTGTA
      541 ATTTTTAGTA CGCTTCGTTC GCTGATCGAC AAGTTAAAAG AATCATTTAA TTCGTCGGAG
      601 ATTAATAGCC TTACCTTGAT TAAGAAAATC AAGACGGTCG ACTTCTTATT TTTGGACGAC
      661 ATCGGAGGAG AGAATTTGAG CTTATGGGCT CGTGACGACT TCTTGTTCGA GGTCCTGAAC
      721 TACCGCATGG AAAACCAGAA GCCAACTTTT TTCACATCTA ATTTTTCAAT TGATCTGTTG
      781 GAGAAGAATT TGCAATTCAC TAAGCAATAT AACATCTTTT TAACCACGCA AGATGTCTTT
      841 AAGTTAGAGA AGATCAAGAT TGATCGTTTA ATTTCCCGCA TCAAAACATT AGCCAAAGAG
      901 ATCAATCTTA CGGGAAAAAA CAAACGTCAA ACTAACTGAA GAGCTTAGAG ACCGGTACTA
      961 GTTCCTGCAG GGCTCGAGGT GTCAATCGTC GGAGCCGCTG AGCAATAACT AGCATAACCC
     1021 CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCATG GTCATAGCTG TTTCCTGAGA
     1081 GCTTGGCAGG TGATGACACA CATTAACAAA TTTCGTGAGG AGTCTCCAGA AGAATGCCAT
     1141 TAATTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG
     1201 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT
     1261 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG
     1321 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG
     1381 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG
     1441 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC
     1501 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG
     1561 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT
     1621 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG
     1681 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC
     1741 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT
     1801 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT
     1861 TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG
     1921 TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT
     1981 CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC
     2041 GCGAGAACCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC
     2101 CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG
     2161 GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC
     2221 AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG
     2281 ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC
     2341 TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT
     2401 GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC
     2461 AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT
     2521 ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC
     2581 TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC
     2641 TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA
     2701 AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT
     2761 CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG
     2821 ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CCTGCACCAG
     2881 TCAGTAAAAC GACGGCCAGT AGTCAAAAGC CTCCGACCGG AGGCTTTTGA CTTGGTCAGG
     2941 TGGAGTGAAT TCGCGGCCGC TTCTAGAGGC TCTTCGTAAT TACGAAGAGG TCTCTA
//