LOCUS       Exported     3242 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3242
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1255..1302
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3130..3146
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3147..3178
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /label="MMSYN1_0609"
                     /note=""
                     /translation="MLSKNFSYSVSLNFELDQEQYKSLTCLYQPLISAQAISLYLTLIQEVRISNILKEEALESKRLLNITNFSYKELIKTLDLLNAFKLIKVYVKKSDYSLIKFEILAPLKSDEFFNHTYLNNLLLNKLEANDYEITKFMLVNETKINTNQYQQIVVDLTDIYDQSLIADINIFDTEVNNFNTYLKKLNQLINVDYVLNSLKDKDIDLDFIQQSTLKSLYDLLTIKKLSEDQIIYLITNSYDFINKNIDINIFKKLLINLITKKETNFDNKELLDLINQTTWSEYSKKKYDIDLSSYTTVFENIKQNYCLSNGIINCLIDFSYKKNNGQIIVKYIAKIAKTLFDKNINTTLKVMQFLKNIQSKSINYNYETMFDSNDFNLQTEAIFEFSEEELKCLV"
     primer_bind     complement(1307..1323)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1390..1978)
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /label="ori"
     CDS             complement(2149..3009)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3010..3114)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /label="MMSYN1_0609"
                     /note=""
                     /translation="MLSKNFSYSVSLNFELDQEQYKSLTCLYQPLISAQAISLYLTLIQEVRISNILKEEALESKRLLNITNFSYKELIKTLDLLNAFKLIKVYVKKSDYSLIKFEILAPLKSDEFFNHTYLNNLLLNKLEANDYEITKFMLVNETKINTNQYQQIVVDLTDIYDQSLIADINIFDTEVNNFNTYLKKLNQLINVDYVLNSLKDKDIDLDFIQQSTLKSLYDLLTIKKLSEDQIIYLITNSYDFINKNIDINIFKKLLINLITKKETNFDNKELLDLINQTTWSEYSKKKYDIDLSSYTTVFENIKQNYCLSNGIINCLIDFSYKKNNGQIIVKYIAKIAKTLFDKNINTTLKVMQFLKNIQSKSINYNYETMFDSNDFNLQTEAIFEFSEEELKCLV"
ORIGIN
        1 ATGTTGTCGA AAAATTTCTC ATACTCAGTC TCGCTGAACT TTGAATTAGA CCAGGAACAG
       61 TACAAATCCC TGACGTGCTT GTACCAACCC TTAATCTCTG CACAGGCCAT TTCGTTATAC
      121 TTAACTTTAA TCCAAGAGGT CCGCATTAGT AACATTCTTA AGGAAGAAGC TCTGGAATCG
      181 AAGCGTCTGT TAAACATCAC CAACTTTAGC TACAAGGAAT TAATCAAGAC ACTGGATTTG
      241 CTTAACGCGT TTAAGCTTAT CAAAGTGTAC GTTAAGAAAA GCGACTACAG TCTGATTAAA
      301 TTCGAAATCC TGGCACCTCT GAAATCTGAT GAATTCTTTA ACCATACTTA CCTTAACAAT
      361 CTTCTGTTAA ACAAGCTTGA AGCGAATGAT TATGAGATCA CCAAGTTCAT GCTGGTTAAC
      421 GAAACGAAAA TCAACACAAA CCAATACCAG CAAATCGTCG TCGATTTGAC CGATATCTAC
      481 GATCAGTCTT TAATTGCTGA CATTAATATC TTTGACACTG AAGTAAATAA TTTCAACACA
      541 TACTTGAAGA AGTTAAATCA GTTAATCAAC GTTGACTATG TCCTGAATAG TCTTAAAGAC
      601 AAAGATATTG ACCTGGATTT TATTCAACAA TCCACGTTAA AGAGCTTATA CGATCTGTTA
      661 ACTATTAAAA AATTATCGGA GGACCAAATT ATTTACCTGA TCACTAATTC ATACGACTTT
      721 ATCAATAAAA ACATTGATAT CAATATCTTC AAAAAACTTC TGATCAATCT TATTACCAAG
      781 AAAGAAACTA ATTTCGACAA CAAGGAATTA CTGGACTTGA TTAATCAGAC AACATGGTCA
      841 GAGTACTCGA AGAAAAAGTA TGATATCGAT TTGTCAAGTT ACACAACGGT GTTCGAGAAT
      901 ATCAAGCAAA ACTACTGCCT TTCTAACGGA ATCATCAACT GTCTGATCGA TTTTTCGTAT
      961 AAGAAAAATA ACGGGCAAAT CATCGTGAAA TATATTGCCA AAATCGCAAA AACCTTATTC
     1021 GACAAAAATA TTAACACAAC CCTTAAGGTT ATGCAATTCT TGAAGAATAT TCAATCAAAA
     1081 TCAATCAATT ACAATTACGA AACCATGTTT GACAGCAACG ACTTTAATTT ACAAACTGAA
     1141 GCAATTTTTG AGTTTAGTGA AGAGGAGCTT AAGTGTTTGG TGTGAAGAGC TTAGAGACCG
     1201 GTACTAGTTC CTGCAGGGCT CGAGGTGTCA ATCGTCGGAG CCGCTGAGCA ATAACTAGCA
     1261 TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCATGGTCA TAGCTGTTTC
     1321 CTGAGAGCTT GGCAGGTGAT GACACACATT AACAAATTTC GTGAGGAGTC TCCAGAAGAA
     1381 TGCCATTAAT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG
     1441 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC
     1501 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC
     1561 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT
     1621 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT
     1681 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC
     1741 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA
     1801 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA
     1861 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG
     1921 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA
     1981 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG
     2041 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG
     2101 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT
     2161 AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT
     2221 CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT
     2281 GATACCGCGA GAACCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG
     2341 AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG
     2401 TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT
     2461 TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC
     2521 CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT
     2581 CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC
     2641 AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA
     2701 GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC
     2761 GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA
     2821 ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA
     2881 ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG
     2941 AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG
     3001 AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT
     3061 GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACCTG
     3121 CACCAGTCAG TAAAACGACG GCCAGTAGTC AAAAGCCTCC GACCGGAGGC TTTTGACTTG
     3181 GTCAGGTGGA GTGAATTCGC GGCCGCTTCT AGAGGCTCTT CGTAATTACG AAGAGGTCTC
     3241 TA
//