LOCUS       Exported     4793 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4793
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
                     /label="T7 terminator"
     terminator      2806..2853
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     4681..4697
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      4698..4729
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /label="MMSYN1_0611"
                     /note="PolA: DNA polymerase I"
                     /translation="MKTKILVVDGNSLIFRAFYATAYSPNTSLLKTKSGVLTNAVYSFINMLLSVIHQRGPYDHILIAFDKGKKTFRHDLLSDYKANRIKTPNELVEQFSVVREFLTKANIQWFEQENIEADDIVGSICKYAEKQFDNLQAEILSSDKDMYQLITNKVICLNPVQGVNELEEVDTNKLFEKWQILPNQVPDYKAIVGDSSDNLKGVNGIGQKGAIKLIQQYQNLENIYNNLEQIKGAIKTKLEQDKKMAFLCKDLATIKTDVILENFSFKKLDFNVDNIYEFLNKYEMYSLKKRFTNILNLDFNPYQNKKQNLDVKIINSWSKDYEDSINYLYVESLEEDYHKDKIIGIGISNNKGNFYLDFKNKAQQLSFFEDTTLSSTDSLFEEFLNNSNLKKYTYDIKKTTYLLKNHKYNVLASNFDFDFMVACYSLNANVVSDLSNQIKLVDNLIELETIDQIFGKGVKKNPDIDLDIKSKYISKKAYLLKKYSDQLIEQLKQTNTYDLYLKIDHPLIEVLYDIEVQGILIDKEQLKLQTQQILKKINHIEGQMKILVAEEIDNNFNFSSPKQIQELLFDKLKLPNLEKGTTSKEVLEKLITYHPIINLLLEHRKYTKLYTTYLKGFEKFIFDDSKVHTIFNHTLTNTGRLSSSYPNIQNISIRDNEQKEVRKIFITNNNKTFLSYDYSQIELRVLAQMSKETNLINAFNQNADIHLQAAKLIFNLSDDQITSEQRRIAKVFNFGILYGLTDFGLASDLNISVNQAKQMIKDYYSAFPSLLEFKEKQVEIATSQGYITTLSNRRRYINELNSTNHNIRQFGKRIAVNTPIQGTASDILKVAMISIYKKLKEQNLDARIVCQIHDEIILEVDDNQLEQTKRIVVSELENALEKLFLDLNIKEQVVVKLKVGESVGKTWFDLK"
     primer_bind     complement(2858..2874)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(2941..3529)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(3700..4560)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
     promoter        complement(4561..4665)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /label="MMSYN1_0611"
                     /note="PolA: DNA polymerase I"
                     /translation="MKTKILVVDGNSLIFRAFYATAYSPNTSLLKTKSGVLTNAVYSFINMLLSVIHQRGPYDHILIAFDKGKKTFRHDLLSDYKANRIKTPNELVEQFSVVREFLTKANIQWFEQENIEADDIVGSICKYAEKQFDNLQAEILSSDKDMYQLITNKVICLNPVQGVNELEEVDTNKLFEKWQILPNQVPDYKAIVGDSSDNLKGVNGIGQKGAIKLIQQYQNLENIYNNLEQIKGAIKTKLEQDKKMAFLCKDLATIKTDVILENFSFKKLDFNVDNIYEFLNKYEMYSLKKRFTNILNLDFNPYQNKKQNLDVKIINSWSKDYEDSINYLYVESLEEDYHKDKIIGIGISNNKGNFYLDFKNKAQQLSFFEDTTLSSTDSLFEEFLNNSNLKKYTYDIKKTTYLLKNHKYNVLASNFDFDFMVACYSLNANVVSDLSNQIKLVDNLIELETIDQIFGKGVKKNPDIDLDIKSKYISKKAYLLKKYSDQLIEQLKQTNTYDLYLKIDHPLIEVLYDIEVQGILIDKEQLKLQTQQILKKINHIEGQMKILVAEEIDNNFNFSSPKQIQELLFDKLKLPNLEKGTTSKEVLEKLITYHPIINLLLEHRKYTKLYTTYLKGFEKFIFDDSKVHTIFNHTLTNTGRLSSSYPNIQNISIRDNEQKEVRKIFITNNNKTFLSYDYSQIELRVLAQMSKETNLINAFNQNADIHLQAAKLIFNLSDDQITSEQRRIAKVFNFGILYGLTDFGLASDLNISVNQAKQMIKDYYSAFPSLLEFKEKQVEIATSQGYITTLSNRRRYINELNSTNHNIRQFGKRIAVNTPIQGTASDILKVAMISIYKKLKEQNLDARIVCQIHDEIILEVDDNQLEQTKRIVVSELENALEKLFLDLNIKEQVVVKLKVGESVGKTWFDLK"
ORIGIN
        1 ATGAAAACGA AAATTCTGGT AGTCGATGGT AATTCACTGA TTTTTCGCGC TTTCTACGCA
       61 ACCGCCTATT CGCCCAATAC TAGCTTACTT AAAACTAAGA GCGGTGTATT AACCAACGCC
      121 GTGTACTCAT TCATTAATAT GTTACTTAGC GTCATTCACC AGCGCGGGCC TTATGATCAT
      181 ATTCTTATTG CATTTGACAA GGGGAAGAAA ACATTCCGTC ATGATCTGCT TTCAGACTAT
      241 AAGGCCAACC GCATTAAAAC ACCGAACGAA TTAGTTGAGC AGTTTTCAGT CGTACGTGAA
      301 TTTCTGACGA AGGCGAATAT CCAATGGTTC GAACAAGAAA ACATTGAGGC AGACGATATT
      361 GTAGGATCCA TCTGTAAGTA CGCCGAAAAA CAGTTCGACA ATCTTCAAGC TGAAATTTTA
      421 TCATCAGATA AAGATATGTA TCAACTGATT ACTAATAAGG TCATTTGTCT GAATCCGGTC
      481 CAGGGTGTCA ATGAACTGGA AGAAGTGGAT ACAAACAAGT TGTTCGAAAA ATGGCAGATT
      541 TTACCGAACC AAGTCCCAGA TTACAAAGCC ATCGTGGGCG ACAGCTCCGA CAATCTTAAA
      601 GGTGTCAACG GAATTGGCCA GAAAGGGGCT ATTAAGTTGA TCCAGCAGTA TCAAAACCTT
      661 GAGAACATTT ACAATAATTT AGAGCAAATC AAGGGGGCTA TCAAGACTAA GTTGGAGCAG
      721 GATAAAAAGA TGGCGTTCTT ATGCAAAGAC CTTGCGACGA TCAAAACCGA TGTCATCTTA
      781 GAGAACTTTT CGTTTAAAAA GTTGGATTTC AACGTCGATA ATATCTACGA ATTCTTGAAC
      841 AAGTATGAAA TGTACTCACT GAAAAAGCGT TTTACCAATA TTTTAAATTT GGACTTCAAT
      901 CCTTACCAGA ATAAGAAACA GAACCTGGAC GTCAAAATCA TCAATTCCTG GTCCAAAGAT
      961 TATGAGGATT CAATTAACTA CCTTTATGTA GAAAGTCTGG AGGAGGACTA CCACAAAGAC
     1021 AAAATTATCG GGATTGGAAT TTCGAACAAC AAGGGAAACT TCTACTTGGA TTTCAAGAAC
     1081 AAGGCGCAAC AGTTATCGTT TTTCGAGGAC ACCACGCTTA GTTCTACAGA CTCGTTATTC
     1141 GAAGAATTCC TTAATAACAG CAACCTCAAG AAATATACAT ACGATATTAA GAAAACTACA
     1201 TATCTGTTAA AGAACCACAA GTACAACGTA CTTGCGTCAA ATTTTGATTT CGACTTCATG
     1261 GTGGCGTGTT ACTCACTTAA CGCGAACGTA GTCTCAGACT TGTCAAATCA AATTAAGTTA
     1321 GTCGATAACT TGATTGAATT GGAAACTATC GATCAGATCT TCGGGAAAGG TGTCAAAAAG
     1381 AACCCCGATA TTGATTTGGA CATCAAGAGC AAATACATTT CGAAAAAGGC GTATTTGCTG
     1441 AAGAAATATA GTGATCAGTT GATTGAGCAG TTGAAACAAA CCAACACTTA CGACTTATAC
     1501 TTGAAGATTG ACCACCCACT TATTGAAGTA CTTTATGACA TCGAAGTCCA AGGGATCTTG
     1561 ATCGATAAAG AACAACTTAA GTTACAAACA CAACAGATCC TGAAGAAAAT CAATCATATC
     1621 GAGGGCCAGA TGAAAATTTT GGTGGCAGAG GAGATTGACA ATAATTTTAA TTTTAGCAGT
     1681 CCCAAGCAAA TTCAGGAGTT GCTTTTTGAC AAGCTGAAGC TGCCGAATCT GGAAAAGGGT
     1741 ACGACTAGTA AAGAGGTATT GGAGAAACTG ATCACATATC ATCCCATCAT CAATTTGCTT
     1801 TTGGAGCACC GTAAATACAC GAAGTTGTAC ACGACGTATC TTAAAGGCTT CGAAAAATTT
     1861 ATCTTCGATG ATTCGAAGGT CCATACGATT TTTAACCACA CCTTGACTAA CACAGGCCGT
     1921 TTATCCAGCT CATACCCTAA TATCCAGAAT ATTTCGATCC GCGACAACGA ACAGAAGGAA
     1981 GTTCGCAAAA TCTTCATTAC TAACAACAAC AAAACCTTTT TATCCTATGA TTATAGTCAA
     2041 ATTGAGCTTC GTGTGCTTGC ACAGATGAGT AAAGAAACGA ATTTGATTAA CGCATTCAAT
     2101 CAAAATGCAG ATATTCATCT GCAGGCCGCA AAGCTGATCT TCAACTTGAG CGACGACCAA
     2161 ATTACCTCTG AACAACGCCG TATTGCTAAA GTTTTCAATT TCGGGATCCT GTACGGCCTT
     2221 ACAGATTTTG GACTGGCCTC AGACTTGAAC ATTTCCGTAA ATCAGGCAAA GCAGATGATT
     2281 AAAGACTATT ACAGCGCATT CCCATCTTTA TTAGAATTTA AGGAAAAACA GGTGGAAATC
     2341 GCTACATCCC AAGGCTACAT TACAACATTA TCCAACCGTC GTCGCTATAT CAACGAGTTG
     2401 AATTCTACCA ACCACAATAT TCGCCAATTT GGAAAGCGTA TCGCCGTCAA TACTCCAATT
     2461 CAGGGAACAG CCAGTGATAT CTTAAAAGTA GCAATGATCT CCATCTATAA GAAGCTTAAA
     2521 GAACAAAACT TGGACGCCCG CATCGTCTGT CAGATTCATG ATGAAATTAT CTTGGAGGTT
     2581 GATGATAATC AACTGGAGCA GACAAAGCGC ATCGTAGTCT CAGAGTTGGA AAACGCACTG
     2641 GAGAAGTTAT TTTTAGACTT AAACATCAAG GAACAGGTGG TTGTAAAACT GAAGGTCGGC
     2701 GAATCAGTGG GCAAAACTTG GTTCGACTTG AAGTGAAGAG CTTAGAGACC GGTACTAGTT
     2761 CCTGCAGGGC TCGAGGTGTC AATCGTCGGA GCCGCTGAGC AATAACTAGC ATAACCCCTT
     2821 GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCATGGTC ATAGCTGTTT CCTGAGAGCT
     2881 TGGCAGGTGA TGACACACAT TAACAAATTT CGTGAGGAGT CTCCAGAAGA ATGCCATTAA
     2941 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG
     3001 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG
     3061 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG
     3121 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC
     3181 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA
     3241 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG
     3301 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC
     3361 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC
     3421 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG
     3481 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT
     3541 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT
     3601 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA
     3661 ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA
     3721 GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT
     3781 GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG
     3841 AGAACCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA
     3901 GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA
     3961 AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG
     4021 CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC
     4081 AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC
     4141 GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA
     4201 TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC
     4261 CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG
     4321 GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC
     4381 GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG
     4441 TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC
     4501 AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT
     4561 ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA
     4621 CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACCT GCACCAGTCA
     4681 GTAAAACGAC GGCCAGTAGT CAAAAGCCTC CGACCGGAGG CTTTTGACTT GGTCAGGTGG
     4741 AGTGAATTCG CGGCCGCTTC TAGAGGCTCT TCGTAATTAC GAAGAGGTCT CTA
//