LOCUS       Exported     4987 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4987
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2010..4973
                     /label="MMSYN1_0612"
                     /note="DnaE: DNA polymerase III subunit alpha"
                     /translation="MNYISLLTIKNQYDFLESLITIDQYIEFIKKNKLNYAFYSETHTMYGVAEFFKKATDNNIKPIIGLTIEFEDSTKLIIYAKNKKGYQILNFVSSFLNDGFNHYDYEIKEYILELVNNNVVVVGLINDLDFKSYLINKLNNDFYDVKELNLYFNQISYLDINDQKTYNILNAIKTNKTINQIQNINNYFYPDIDYLIKNYSLENIKKVISEINSKVDFNLFDSNQKHLVKYKNINNLSSYEYLRQVCLLSLKKYQQKIKPNLDLKLYINRLNYELEIIKQMGFSDYFLIVSDYVNFAKKNDILVGPGRGSAAGSLISYLLRITDIDPLEYDLLFERFLNPDRSNLPDIDLDFQDNRREEVLEYLFEKYGKYHVGMITTYQTIGYKMAWRDVCRVFNIDLLIVNKISKVLDQYTNSDFLEFIKENKLLNDYFQNDLFKEIFITMHKIIGLPRQTSTHAAGIVLTDCDLRELVPIKIGFNGINQTQFDMNYLDALGLIKMDILGLRNLTTIQEIKHLIYLNQNLKISLNKIDLNDKKAFELLKNKQTSGIFQLESKGMSDLISKMQVDSIEQISIASALYRPGPQEMIPIYLENKKTNKFKIIDKSVYEILKPTYGIIVYQEQVMQMLNKVANFSYAKADIIRRAMSKKNNKVMQSMKLEFINSAIKNNFSYNKANLIWNWIEKFSNYGFNKSHSISYSYISYWLAYFKAHFTTEFYTSLLDQNIGNEIKTQQYIKELYDYKIKVNKPSVINSNFNYQIINKQIYMPLTCIKSIGYEVVKKINLAKSENENMYLDIHNFILAMIKQKINVNVLQTLIKAGALDIFNYNKKTMIENLDLLISQANAYKQVNNILDDEKINLIIYDEYEDEILASFEKELYGFFIDQNPILKLKTNNFNLNLIDISKLEYNKVQVILGYILKIKEIKDKNNNKMAFVTVFDNTSELELTIFSSDYKDISDQLIENKAYVFKVLKTKTNNKTSIKFISLIKAI"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GGGTCTCAAA TGAATTATAT TTCCCTTCTG ACTATCAAGA
     2041 ACCAATACGA TTTTTTGGAA AGTCTGATCA CGATCGACCA ATATATTGAG TTCATCAAAA
     2101 AGAATAAGTT AAATTACGCG TTTTACTCGG AAACGCACAC GATGTACGGG GTCGCAGAAT
     2161 TTTTTAAGAA AGCAACCGAC AATAATATTA AACCTATTAT CGGCTTAACG ATTGAGTTTG
     2221 AGGACTCCAC AAAGCTTATT ATTTATGCCA AGAATAAAAA GGGATATCAG ATTTTGAATT
     2281 TTGTCAGTAG TTTTTTAAAC GATGGTTTTA ACCACTATGA CTACGAGATC AAGGAATACA
     2341 TTTTAGAATT AGTTAACAAC AATGTAGTAG TGGTAGGTCT TATTAATGAT TTAGACTTTA
     2401 AGTCCTATCT GATTAATAAG TTGAATAATG ACTTCTATGA CGTCAAGGAA CTGAACTTGT
     2461 ATTTCAACCA GATTTCATAT TTAGATATCA ATGATCAGAA AACCTATAAC ATCTTAAATG
     2521 CAATCAAGAC CAACAAAACA ATTAACCAGA TTCAAAACAT TAACAATTAC TTCTATCCAG
     2581 ACATTGATTA TTTAATTAAG AACTACAGTT TAGAGAATAT CAAGAAAGTG ATTTCCGAGA
     2641 TCAATTCGAA AGTGGACTTT AATCTTTTTG ACTCTAATCA AAAACATCTT GTTAAGTATA
     2701 AAAATATCAA TAATCTTAGC AGTTATGAAT ACTTACGTCA GGTGTGTCTG CTTAGCTTGA
     2761 AGAAATATCA GCAGAAGATC AAACCTAATT TAGACTTAAA GTTATACATT AATCGCTTAA
     2821 ATTACGAGTT GGAAATTATC AAGCAAATGG GCTTCTCAGA TTATTTTTTG ATTGTCTCGG
     2881 ATTACGTGAA CTTCGCGAAG AAGAATGATA TCTTGGTCGG GCCGGGACGC GGAAGCGCTG
     2941 CAGGCTCTCT GATCTCGTAT TTATTGCGCA TTACTGATAT CGATCCCTTA GAGTACGATC
     3001 TGCTGTTCGA ACGTTTTTTA AATCCCGATC GCTCCAACTT GCCAGACATT GATTTAGACT
     3061 TTCAAGATAA TCGTCGTGAA GAGGTCCTGG AATACCTTTT CGAGAAGTAC GGTAAATATC
     3121 ATGTCGGAAT GATTACGACG TACCAGACCA TCGGCTATAA AATGGCATGG CGTGACGTAT
     3181 GCCGCGTATT CAACATTGAT TTGCTTATTG TTAATAAGAT CTCAAAAGTG CTTGACCAAT
     3241 ACACAAATAG TGATTTTTTA GAGTTCATTA AGGAGAATAA ATTATTGAAT GACTATTTCC
     3301 AGAATGACCT GTTCAAAGAA ATTTTCATCA CTATGCATAA AATTATTGGA TTACCTCGCC
     3361 AGACTTCCAC TCACGCAGCT GGAATCGTAC TTACCGACTG TGATTTGCGC GAACTTGTGC
     3421 CCATCAAGAT CGGGTTTAAT GGGATCAACC AAACGCAATT CGACATGAAT TATTTGGATG
     3481 CCTTGGGGCT GATTAAAATG GATATCTTGG GCTTACGCAA CCTGACCACA ATTCAAGAAA
     3541 TTAAACACTT GATCTATTTG AATCAGAATC TTAAGATCTC TCTTAACAAA ATCGACTTAA
     3601 ATGATAAGAA AGCGTTCGAG TTACTTAAAA ATAAGCAGAC GAGCGGCATT TTTCAGTTGG
     3661 AGAGTAAGGG CATGAGCGAT TTGATTTCCA AAATGCAAGT TGATTCTATT GAACAAATTT
     3721 CAATTGCTTC CGCTCTGTAT CGTCCAGGGC CGCAAGAAAT GATCCCTATC TACTTAGAGA
     3781 ACAAGAAAAC CAACAAATTT AAAATTATTG ATAAGTCTGT ATATGAGATC CTTAAACCAA
     3841 CTTACGGGAT TATCGTTTAC CAGGAGCAAG TGATGCAAAT GCTTAACAAG GTAGCTAACT
     3901 TCTCGTACGC CAAGGCCGAT ATTATTCGCC GTGCAATGTC TAAGAAAAAC AACAAAGTAA
     3961 TGCAGAGTAT GAAACTTGAG TTTATTAATT CTGCAATTAA GAATAACTTT TCATACAACA
     4021 AAGCCAACTT GATTTGGAAT TGGATTGAGA AGTTCAGTAA TTACGGATTC AACAAATCTC
     4081 ATTCCATTAG CTATAGCTAC ATCAGCTACT GGCTTGCCTA CTTCAAAGCG CACTTCACGA
     4141 CGGAGTTCTA TACCAGCCTG CTGGATCAGA ATATCGGCAA TGAGATTAAG ACACAGCAGT
     4201 ACATCAAGGA GTTGTACGAT TACAAAATTA AGGTCAATAA GCCTTCAGTG ATTAACTCGA
     4261 ATTTTAATTA TCAGATTATC AATAAGCAGA TCTATATGCC ATTGACTTGT ATTAAGTCGA
     4321 TCGGTTACGA GGTTGTTAAG AAAATTAATC TGGCAAAGAG CGAAAATGAG AATATGTACC
     4381 TTGACATCCA CAATTTTATT CTTGCCATGA TTAAACAGAA GATCAACGTT AATGTCCTGC
     4441 AAACACTGAT TAAAGCTGGT GCATTAGACA TCTTTAATTA TAACAAAAAG ACAATGATTG
     4501 AGAACTTGGA TCTGCTTATT TCGCAAGCAA ACGCTTATAA ACAAGTGAAT AACATCTTGG
     4561 ATGATGAAAA GATCAACTTG ATTATTTACG ACGAATACGA GGATGAAATT TTAGCCTCAT
     4621 TCGAAAAAGA GCTTTACGGA TTTTTTATTG ACCAAAACCC AATCTTAAAA CTTAAGACTA
     4681 ACAACTTCAA TCTGAATTTA ATCGATATCA GCAAACTTGA ATATAACAAA GTCCAAGTAA
     4741 TCTTGGGATA TATCCTGAAA ATCAAGGAGA TTAAGGATAA GAACAACAAC AAAATGGCGT
     4801 TTGTAACAGT ATTTGATAAT ACCAGTGAGC TGGAATTAAC AATCTTCTCT TCTGACTACA
     4861 AGGATATTAG TGATCAGCTG ATTGAAAATA AGGCGTACGT ATTCAAGGTC CTTAAGACTA
     4921 AAACCAACAA TAAGACATCA ATCAAGTTTA TTAGTTTGAT CAAAGCGATT TGAAGAGCTT
     4981 AGAGACC
//