LOCUS       Exported     3302 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3302
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1315..1362
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3190..3206
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3207..3238
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
                     /label="tonB terminator"
     CDS             1..0
                     /label="MMSYN1_0613"
                     /note="Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr)."
                     /translation="MKNNILEELKWRGLIKQITNESKILDAQNNSDAVYCGFDPTADSLHVGHLMMIITLKRFADYNFKPIALIGGATGMIGDPSFKANERVLQTKDQVEHNINKISAQLKQIIPNVNFVNNNTWLSSISLIDFLRDIGKHFNLSYLLAKESIATRIQTGLSVTEFCYTMLQAYDFYYLYKNNNCSIQIGGSDQWGNITSGIDFISDTINKNNKAAGLTINLLTKSDGQKFGKTESGAVWLDKTKTSEYEFYQFWFNQTDQDSINLLKCLTFLTKEQIDNLIKEHQNQPSKHLLQKALASEMTKFVHQQQGLDKALKLTEAFFSGDLFSLTDDLFKMALNSLPNTQINKDTKVIDALIEVKAASSKREAREFLTNKAIMINNQIIEDENTLISSFDLIQNKYLLVKKGKKKYFVILIK"
     primer_bind     complement(1367..1383)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1450..2038)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2209..3069)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3070..3174)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /note="Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr)."
                     /translation="MKNNILEELKWRGLIKQITNESKILDAQNNSDAVYCGFDPTADSLHVGHLMMIITLKRFADYNFKPIALIGGATGMIGDPSFKANERVLQTKDQVEHNINKISAQLKQIIPNVNFVNNNTWLSSISLIDFLRDIGKHFNLSYLLAKESIATRIQTGLSVTEFCYTMLQAYDFYYLYKNNNCSIQIGGSDQWGNITSGIDFISDTINKNNKAAGLTINLLTKSDGQKFGKTESGAVWLDKTKTSEYEFYQFWFNQTDQDSINLLKCLTFLTKEQIDNLIKEHQNQPSKHLLQKALASEMTKFVHQQQGLDKALKLTEAFFSGDLFSLTDDLFKMALNSLPNTQINKDTKVIDALIEVKAASSKREAREFLTNKAIMINNQIIEDENTLISSFDLIQNKYLLVKKGKKKYFVILIK"
                     /label="MMSYN1_0613"
ORIGIN
        1 ATGAAGAACA ACATTTTGGA GGAGCTGAAG TGGCGTGGCC TTATCAAACA AATTACTAAT
       61 GAAAGTAAAA TTCTGGATGC CCAGAATAAT TCAGACGCAG TCTACTGTGG CTTTGACCCA
      121 ACCGCTGATT CCCTTCATGT AGGCCACTTA ATGATGATTA TTACGTTGAA GCGTTTTGCA
      181 GATTATAATT TCAAACCCAT TGCACTGATC GGTGGGGCTA CCGGGATGAT TGGCGACCCG
      241 AGTTTTAAAG CAAACGAGCG TGTTTTACAA ACCAAGGACC AGGTTGAGCA TAATATTAAT
      301 AAGATTAGCG CTCAGTTGAA GCAGATCATT CCTAATGTAA ACTTTGTAAA TAACAACACA
      361 TGGCTTTCGT CGATCTCGCT TATCGACTTC CTGCGTGACA TCGGTAAACA TTTCAACTTG
      421 TCTTATCTTC TGGCAAAGGA GTCTATCGCA ACCCGTATTC AAACTGGGCT GTCCGTGACC
      481 GAGTTTTGCT ACACAATGTT GCAGGCTTAC GACTTTTATT ACCTTTACAA AAATAATAAT
      541 TGTTCCATCC AGATCGGAGG ATCGGATCAG TGGGGTAATA TCACAAGTGG GATTGATTTT
      601 ATTAGTGATA CCATTAACAA AAACAATAAG GCAGCGGGTC TGACTATCAA CTTACTTACC
      661 AAGTCAGACG GTCAAAAATT TGGTAAAACG GAGAGCGGAG CCGTATGGCT TGACAAAACT
      721 AAAACTAGTG AGTACGAATT TTACCAGTTT TGGTTCAACC AGACGGACCA AGACTCAATC
      781 AATCTGCTGA AATGTTTGAC GTTCCTGACT AAGGAGCAGA TTGACAACCT TATTAAGGAG
      841 CATCAAAATC AGCCTTCAAA GCATCTGTTA CAGAAAGCGC TGGCTTCGGA AATGACCAAG
      901 TTCGTGCACC AACAGCAGGG CCTTGATAAG GCACTTAAAC TTACTGAGGC GTTTTTCAGC
      961 GGGGACCTTT TCAGTCTTAC AGATGACTTG TTCAAAATGG CACTTAATTC GCTGCCCAAC
     1021 ACGCAGATTA ACAAGGATAC CAAAGTTATC GATGCCTTAA TCGAAGTCAA AGCTGCGTCG
     1081 TCAAAGCGCG AAGCGCGCGA GTTTCTGACT AATAAGGCGA TCATGATCAA TAATCAGATT
     1141 ATCGAGGACG AGAATACGCT TATCAGTTCG TTCGACCTGA TCCAAAATAA GTACCTGTTA
     1201 GTCAAGAAAG GTAAGAAAAA ATACTTTGTT ATTTTGATCA AGTGAAGAGC TTAGAGACCG
     1261 GTACTAGTTC CTGCAGGGCT CGAGGTGTCA ATCGTCGGAG CCGCTGAGCA ATAACTAGCA
     1321 TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCATGGTCA TAGCTGTTTC
     1381 CTGAGAGCTT GGCAGGTGAT GACACACATT AACAAATTTC GTGAGGAGTC TCCAGAAGAA
     1441 TGCCATTAAT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG
     1501 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC
     1561 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC
     1621 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT
     1681 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT
     1741 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC
     1801 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA
     1861 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA
     1921 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG
     1981 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA
     2041 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG
     2101 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG
     2161 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT
     2221 AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT
     2281 CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT
     2341 GATACCGCGA GAACCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG
     2401 AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG
     2461 TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT
     2521 TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC
     2581 CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT
     2641 CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC
     2701 AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA
     2761 GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC
     2821 GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA
     2881 ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA
     2941 ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG
     3001 AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG
     3061 AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT
     3121 GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACCTG
     3181 CACCAGTCAG TAAAACGACG GCCAGTAGTC AAAAGCCTCC GACCGGAGGC TTTTGACTTG
     3241 GTCAGGTGGA GTGAATTCGC GGCCGCTTCT AGAGGCTCTT CGTAATTACG AAGAGGTCTC
     3301 TA
//