LOCUS       Exported     3119 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3119
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1132..1179
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3007..3023
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3024..3055
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
                     /label="tonB terminator"
     CDS             1..0
                     /label="MMSYN1_0614"
                     /note="PncB: Nicotinate phosphoribosyltransferase"
                     /translation="MQNKTKFKFDPRVKDGYFIADYFKKTVEILKNFKHDQIITMQFFQRNDNVVLCGISEVIDLLKFASPNYDDLEIYALNDGDIINSKEPVLKITGRYQDFGWLEGMIDGILSRNTSIATNSKQIIDAANHKDVLNMLDRADNYLTLASDGYASYIGGFRLFVTEASLEYIDDKTVLQPSGTMPHALIQAFNGDTLKAADAFYKTYPNNKLVVLIDYDNDCVNMAAKIGQHFKEKLYAVRLDTSENLVDKFFINNKEYTNQTNLNGVSEQLVREVRKALDNVGCNHTKIIVSSSFSANKIKEFESKNVPVDIYGVGSALAKINIHFTGDAVLINNQKQAKFGRENIENLRLKKVK"
     primer_bind     complement(1184..1200)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1267..1855)
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /label="ori"
     CDS             complement(2026..2886)
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
     promoter        complement(2887..2991)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /label="MMSYN1_0614"
                     /note="PncB: Nicotinate phosphoribosyltransferase"
                     /translation="MQNKTKFKFDPRVKDGYFIADYFKKTVEILKNFKHDQIITMQFFQRNDNVVLCGISEVIDLLKFASPNYDDLEIYALNDGDIINSKEPVLKITGRYQDFGWLEGMIDGILSRNTSIATNSKQIIDAANHKDVLNMLDRADNYLTLASDGYASYIGGFRLFVTEASLEYIDDKTVLQPSGTMPHALIQAFNGDTLKAADAFYKTYPNNKLVVLIDYDNDCVNMAAKIGQHFKEKLYAVRLDTSENLVDKFFINNKEYTNQTNLNGVSEQLVREVRKALDNVGCNHTKIIVSSSFSANKIKEFESKNVPVDIYGVGSALAKINIHFTGDAVLINNQKQAKFGRENIENLRLKKVK"
ORIGIN
        1 ATGCAAAACA AAACTAAGTT CAAGTTCGAC CCACGCGTAA AGGATGGGTA CTTCATAGCG
       61 GATTATTTCA AGAAAACAGT AGAGATTCTT AAAAACTTCA AGCACGACCA GATAATAACA
      121 ATGCAGTTCT TCCAACGTAA TGATAACGTG GTATTATGCG GAATCAGTGA AGTGATTGAT
      181 CTGTTGAAGT TTGCCTCCCC TAATTATGAC GACTTGGAGA TCTATGCTCT GAACGATGGT
      241 GATATAATAA ACTCGAAGGA ACCGGTCTTG AAGATAACTG GACGCTATCA AGACTTTGGT
      301 TGGTTAGAAG GCATGATAGA TGGCATTCTT AGTCGTAACA CGAGTATCGC AACGAATTCC
      361 AAGCAGATCA TTGACGCTGC CAATCATAAA GATGTGCTTA ATATGTTGGA TCGGGCAGAC
      421 AATTACTTGA CGTTAGCGTC GGACGGATAT GCAAGTTACA TCGGCGGATT CCGTCTTTTC
      481 GTCACCGAAG CTTCACTGGA GTACATCGAT GACAAGACGG TGCTTCAACC GAGTGGAACA
      541 ATGCCACACG CACTGATACA AGCATTCAAC GGGGATACGC TTAAAGCAGC CGATGCCTTT
      601 TATAAAACCT ATCCGAACAA CAAATTGGTC GTGCTGATCG ATTACGATAA CGATTGCGTC
      661 AATATGGCTG CGAAAATCGG ACAGCATTTC AAAGAAAAAT TATATGCTGT GCGTTTGGAT
      721 ACCAGTGAAA ATCTTGTCGA TAAGTTCTTT ATCAATAATA AGGAATACAC AAACCAGACT
      781 AATTTAAATG GCGTTTCTGA GCAGCTGGTC CGGGAAGTTC GCAAGGCGCT GGATAATGTC
      841 GGCTGCAATC ACACCAAAAT TATCGTTAGT TCATCCTTTT CTGCCAACAA AATAAAAGAA
      901 TTCGAGTCCA AAAACGTTCC TGTCGATATC TACGGTGTCG GCTCTGCCCT GGCCAAAATA
      961 AATATCCATT TTACCGGGGA TGCTGTTCTG ATTAATAATC AAAAGCAAGC AAAGTTTGGT
     1021 AGAGAAAATA TCGAGAATCT GAGACTGAAA AAAGTAAAGT GAAGAGCTTA GAGACCGGTA
     1081 CTAGTTCCTG CAGGGCTCGA GGTGTCAATC GTCGGAGCCG CTGAGCAATA ACTAGCATAA
     1141 CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC ATGGTCATAG CTGTTTCCTG
     1201 AGAGCTTGGC AGGTGATGAC ACACATTAAC AAATTTCGTG AGGAGTCTCC AGAAGAATGC
     1261 CATTAATTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA
     1321 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT
     1381 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC
     1441 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT
     1501 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC
     1561 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC
     1621 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG
     1681 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC
     1741 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG
     1801 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA
     1861 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT
     1921 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG
     1981 TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT
     2041 CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC
     2101 CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT
     2161 ACCGCGAGAA CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG
     2221 GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG
     2281 CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC
     2341 TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA
     2401 ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG
     2461 TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC
     2521 ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA
     2581 CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC
     2641 AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG
     2701 TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC
     2761 CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC
     2821 AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT
     2881 ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG
     2941 CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACCTGCAC
     3001 CAGTCAGTAA AACGACGGCC AGTAGTCAAA AGCCTCCGAC CGGAGGCTTT TGACTTGGTC
     3061 AGGTGGAGTG AATTCGCGGC CGCTTCTAGA GGCTCTTCGT AATTACGAAG AGGTCTCTA
//