LOCUS Exported 2909 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..2909 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 922..969 /note="transcription terminator for bacteriophage T7 RNA polymerase" /label="T7 terminator" primer_bind 2797..2813 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 2814..2845 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 1..0 /note="" /translation="MKFGILVDSAAVYDPAEFKNTIIDVIPLHIVFPNNDEYLDIKNIVEQEKILEKVSMGENIKTSQASPGELEKKYDELLEQYEHIIHIPITNNLSSMLQTATLVSQDEKYKDKITVYQNNDLAAQGIALTALSLAKAIKSNKIKTAQQAIDFIENFKEKVLIAIIPGDLKKLSNGGRAKGVITTVLNLLKTKLLIIWAKEPKKEAIGRTYNSLIEKLIKNLSNKFKKNKYKLYFLSTPLTSSKTVEIVKQILSDEKINFVHGNVPNIYTIHAGVETIGFVAIEE" /label="MMSYN1_0617" primer_bind complement(974..990) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" rep_origin complement(1057..1645) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(1816..2676) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(2677..2781) /label="bla promoter" /note="ampicillin resistance gene promoter" CDS complement(1..0) /label="MMSYN1_0617" /note="" /translation="MKFGILVDSAAVYDPAEFKNTIIDVIPLHIVFPNNDEYLDIKNIVEQEKILEKVSMGENIKTSQASPGELEKKYDELLEQYEHIIHIPITNNLSSMLQTATLVSQDEKYKDKITVYQNNDLAAQGIALTALSLAKAIKSNKIKTAQQAIDFIENFKEKVLIAIIPGDLKKLSNGGRAKGVITTVLNLLKTKLLIIWAKEPKKEAIGRTYNSLIEKLIKNLSNKFKKNKYKLYFLSTPLTSSKTVEIVKQILSDEKINFVHGNVPNIYTIHAGVETIGFVAIEE" ORIGIN 1 ATGAAATTTG GTATCCTTGT GGATAGCGCT GCGGTTTACG ACCCAGCTGA ATTCAAAAAC 61 ACGATCATCG ACGTCATCCC ACTGCATATT GTATTCCCAA ACAATGATGA ATACCTGGAT 121 ATCAAAAATA TCGTTGAACA GGAAAAGATC CTGGAAAAGG TATCGATGGG AGAAAATATT 181 AAAACATCCC AAGCGAGCCC AGGAGAGCTG GAGAAAAAAT ATGATGAACT TCTTGAGCAG 241 TATGAACATA TCATTCACAT TCCCATCACG AATAACCTTA GCTCCATGTT ACAGACAGCC 301 ACGCTTGTCT CTCAGGACGA GAAGTATAAA GACAAGATCA CTGTCTATCA AAACAATGAT 361 CTTGCTGCTC AGGGCATTGC ATTAACCGCA TTGTCTCTTG CGAAGGCTAT TAAGTCAAAC 421 AAAATTAAAA CTGCTCAGCA AGCCATTGAC TTTATTGAAA ACTTTAAGGA GAAAGTCCTG 481 ATTGCCATCA TCCCAGGGGA CTTAAAGAAG TTGTCCAATG GTGGTCGTGC CAAAGGAGTG 541 ATCACCACAG TGCTGAACTT ATTAAAGACG AAACTTCTGA TTATTTGGGC AAAGGAGCCC 601 AAGAAAGAGG CTATTGGTCG CACTTATAAT AGCTTGATCG AGAAGCTGAT TAAGAATTTA 661 TCAAACAAGT TTAAGAAGAA TAAGTATAAG CTTTACTTTT TGTCAACGCC TCTGACATCT 721 TCTAAGACTG TGGAAATTGT GAAGCAAATT CTGTCGGACG AGAAAATTAA TTTTGTGCAC 781 GGTAATGTTC CTAATATTTA CACCATTCAT GCCGGTGTCG AGACTATCGG ATTTGTGGCG 841 ATCGAAGAGT GAAGAGCTTA GAGACCGGTA CTAGTTCCTG CAGGGCTCGA GGTGTCAATC 901 GTCGGAGCCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG 961 GGTTTTTTGC ATGGTCATAG CTGTTTCCTG AGAGCTTGGC AGGTGATGAC ACACATTAAC 1021 AAATTTCGTG AGGAGTCTCC AGAAGAATGC CATTAATTTC CATAGGCTCC GCCCCCCTGA 1081 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 1141 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 1201 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 1261 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 1321 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 1381 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 1441 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 1501 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 1561 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 1621 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 1681 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 1741 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 1801 AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT 1861 ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG 1921 CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAA CCACGCTCAC CGGCTCCAGA 1981 TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT 2041 ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT 2101 TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT 2161 TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT 2221 GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC 2281 CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC 2341 CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT 2401 GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG 2461 AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT 2521 ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC 2581 TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA 2641 GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG 2701 AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA 2761 TAAACAAATA GGGGTTCCGC GCACCTGCAC CAGTCAGTAA AACGACGGCC AGTAGTCAAA 2821 AGCCTCCGAC CGGAGGCTTT TGACTTGGTC AGGTGGAGTG AATTCGCGGC CGCTTCTAGA 2881 GGCTCTTCGT AATTACGAAG AGGTCTCTA //