LOCUS       Exported     4438 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4438
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2010..4424
                     /translation="MDFSHKAIEKKWQKYWKENNIYKTTNNSENKAYILDMFPYPSGSGLHVGHIKGYTATDVYSRFKRMQGYDVLHPIGWDAFGLPAEQYALKTGNDPREFTLQNIENFKVQLNKMGFSYDYDKEINTADPNYYKTTQWIFKQLYKKGLAENRDIDVNWCQELGTVLANDEIIEKNGLMVSERGEYPVVKKKMRQWVLKITDYADKLLDGLDNLDWPNSVKELQRNWIGKSEGCEINFKSNDINIPVFTTRADTIFGATYIVLAPENELVLKLTTPEKLDEVKKYIELTANKSEIERKDESRTKTGVFIGSYATNPLTKEQIQIWISDYVLNDYGSGAIMAVPAHDKRDWDFATKFNLPIRFVISTKDESKAFVGEGIHINSEFLNDLDRVQALQVIHNYVEKNNLGKKKINYKLRDWLFSRQRFYGEPFPVLYDKNNNIVLIEDDNLPITLPTTDYIKPTNTGESPLANVRNWVNVKIGDKEYKRETNTMPQSAGSSWYFIAYILADSKNNLIDLTSDEAKKRLEKWLPVDLYIGGQEHAVGHLLYARFWTHFLYDLGLLPTNEPFKRLFNQGMILGPDNRKMSKSWGNVINPDDVIDTHGADALRLYEMFMGPLDASLPWSFDGLDASLKWLNRCYRMINKIEFSNTNNHKLDYVYNDVVKKVTQMIQELKFNTAISQLMVLVNAIYKEELNTVYKPYIEGFVKMLSLFAPHLSEELWEKLGNNSSVTLQTWPEFDETKIIKNTVVIALQVNGKLRSTIEVEKGTDKETLINLAEKNENIIKFIKDHKILKRIAVIDRIVNIVIE"
                     /label="MMSYN1_0634"
                     /note=""
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /note="ampicillin resistance gene promoter"
                     /label="bla promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GGGTCTCAAA TGGACTTCTC TCATAAAGCG ATTGAGAAGA
     2041 AATGGCAAAA GTACTGGAAG GAGAATAATA TTTACAAAAC AACGAATAAC AGCGAAAATA
     2101 AGGCGTATAT TCTTGACATG TTCCCTTACC CTAGTGGGTC GGGACTGCAC GTGGGCCATA
     2161 TCAAGGGGTA TACTGCTACG GACGTTTACT CTCGCTTCAA GCGCATGCAA GGATACGATG
     2221 TACTGCACCC TATTGGATGG GACGCGTTTG GGCTGCCCGC CGAGCAATAT GCGTTGAAAA
     2281 CCGGCAACGA CCCCCGCGAG TTCACCTTAC AGAACATTGA AAACTTCAAA GTCCAACTGA
     2341 ATAAGATGGG CTTCAGTTAT GATTATGATA AGGAGATTAA TACAGCAGAC CCCAACTACT
     2401 ACAAAACTAC ACAATGGATC TTCAAACAAT TGTACAAGAA AGGCTTAGCC GAAAATCGCG
     2461 ATATTGACGT GAACTGGTGC CAAGAATTGG GGACCGTGCT GGCGAATGAC GAGATTATCG
     2521 AGAAAAACGG TTTAATGGTT TCTGAGCGCG GCGAGTATCC GGTTGTTAAA AAGAAGATGC
     2581 GTCAGTGGGT CCTGAAGATC ACAGATTATG CAGACAAACT GTTAGATGGC TTAGACAACC
     2641 TGGATTGGCC AAATTCGGTG AAAGAGTTAC AGCGCAACTG GATTGGGAAA AGTGAAGGGT
     2701 GTGAGATTAA CTTCAAATCA AATGACATTA ATATTCCGGT TTTTACGACC CGCGCGGACA
     2761 CGATTTTTGG CGCGACGTAT ATTGTATTGG CTCCTGAAAA TGAATTGGTG CTGAAGCTGA
     2821 CAACCCCTGA GAAGTTAGAT GAGGTAAAGA AGTATATCGA ATTGACCGCC AACAAGTCAG
     2881 AGATTGAACG CAAAGATGAG TCACGCACTA AAACCGGTGT ATTCATTGGC AGTTATGCTA
     2941 CGAATCCTCT TACAAAGGAG CAAATTCAGA TTTGGATTTC GGATTACGTA CTGAATGATT
     3001 ATGGATCTGG TGCAATCATG GCAGTCCCTG CGCACGACAA ACGCGATTGG GATTTTGCAA
     3061 CTAAATTTAA TTTACCTATC CGTTTCGTGA TTAGCACCAA AGATGAGTCC AAAGCGTTTG
     3121 TCGGCGAAGG GATTCATATT AACTCGGAGT TTTTGAATGA CTTGGATCGC GTACAAGCCC
     3181 TTCAGGTAAT CCATAACTAC GTTGAGAAAA ACAATTTAGG CAAGAAGAAA ATCAATTATA
     3241 AACTTCGTGA CTGGTTGTTT AGCCGTCAGC GTTTCTATGG GGAACCATTT CCAGTCCTGT
     3301 ACGACAAAAA TAATAACATT GTCCTTATTG AGGACGATAA TCTGCCCATT ACCTTGCCAA
     3361 CTACTGACTA TATCAAACCA ACGAATACGG GCGAGAGCCC ATTAGCAAAC GTGCGCAACT
     3421 GGGTCAACGT TAAAATCGGA GATAAGGAGT ATAAACGTGA AACGAATACA ATGCCCCAGT
     3481 CCGCTGGGAG TAGCTGGTAC TTCATTGCAT ATATTCTTGC GGATAGCAAG AATAACCTGA
     3541 TCGATTTGAC CTCAGATGAG GCTAAAAAGC GTCTGGAAAA GTGGTTACCC GTCGATTTAT
     3601 ATATTGGGGG TCAGGAACAT GCAGTAGGTC ATCTGCTTTA CGCACGTTTT TGGACGCACT
     3661 TCTTGTATGA CCTTGGCCTG CTGCCAACTA ACGAGCCTTT CAAGCGTTTA TTTAATCAGG
     3721 GCATGATTCT TGGCCCAGAT AACCGCAAGA TGAGCAAAAG TTGGGGAAAT GTGATTAACC
     3781 CCGACGATGT GATTGATACA CATGGCGCTG ATGCGCTTCG CTTGTATGAG ATGTTCATGG
     3841 GGCCCTTGGA CGCGTCGCTT CCCTGGTCGT TTGATGGGCT TGATGCCTCC CTTAAGTGGT
     3901 TGAACCGCTG TTATCGCATG ATCAACAAAA TTGAGTTCTC GAACACCAAC AACCACAAGT
     3961 TGGATTACGT ATACAACGAT GTGGTGAAAA AAGTCACTCA AATGATCCAA GAATTAAAAT
     4021 TTAATACTGC GATTTCGCAA TTGATGGTGC TTGTCAACGC GATTTACAAA GAGGAGCTTA
     4081 ACACCGTATA CAAACCTTAT ATCGAGGGTT TTGTTAAGAT GCTTAGTTTA TTCGCACCAC
     4141 ACTTGTCAGA AGAATTATGG GAGAAGCTTG GTAACAACTC ATCGGTAACC CTGCAAACAT
     4201 GGCCCGAGTT TGACGAGACT AAAATTATCA AAAATACTGT TGTGATCGCA CTTCAGGTAA
     4261 ACGGAAAGCT TCGTTCGACA ATCGAAGTAG AAAAAGGGAC AGACAAAGAA ACCTTAATCA
     4321 ATCTTGCCGA AAAGAATGAG AACATTATCA AATTTATCAA GGACCATAAG ATCTTAAAAC
     4381 GTATTGCGGT TATTGACCGC ATCGTCAACA TCGTCATTGA GTGAAGAGCT TAGAGACC
//