LOCUS       Exported     4813 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4813
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
                     /label="T7 terminator"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
                     /label="tonB terminator"
     CDS             2010..4799
                     /label="MMSYN1_0636"
                     /note="lipoprotein, putative"
                     /translation="MKKILAILSSLTLVSTGVFSTVLSCKKTLTPTTKPNTNNNKVLKNNSLDNIKTISAMLLKQAVLADMYGYNFDFLKSYFNNKNLNEQAKRYKLNTEIKDNITLSTDFEDALANYFSTNLVIKKNDNVNLDGIKGTDIDFLTSVLPKTVFGTTSKQISAAISIILENISGAGITGLLDLAKNIDVNSKFSDFVKNLNVSKELITTLLNTIFTNDKFLKELEEEINKFDALTLYKDFELSELSNLALLNILDGINGILDKDYQLVSSDIKKNNGSTLNVKLWNTSKTFINKVAKFDQTSNVSTISSFSNSTSPTILPTNIKRNIKTAASLIRGLELFQYLFSLFDESRKDEFKISDENIFDKSKKNSEFIKNIYKINGSTGGSNNGSNKIESLNGTSNGSTSKTTLNLKYIIDTLQYYLGNLDKSDKAYRLRQFIAILFSGKYTENIYKPENNNNGNGSNEYKSFFFEFNGAPENKIKEIKLNGFQIFLTSILFESLSNIKLQNIKIESGIFSLAKPFIEKINLKNFFESEVFLKKGLADFLISLMNLITDSFVYNQPLVNDNFDKILENLVTILKTLKFDDLLKALFNETNGIVSSLKSLIEKYVKFEDISKKIDEFIKKKETFSLVKVGIKSFIPILGEKFFEYIYDGKVEQTFDTLANLSNDVLIRTLVEKLKIQIPAALNFILPYFKKIAMSLRTIFPPNVHLNLKNLFTIKLSDFIKLENKPNFGSDYLDKSITTILNELSGADGSGSKLKDLDNAYGFKIDSLKEFINKIFKYDYKWNGKDLENGNLISLLLNNPNKFKEIIGLTEEGMKKDSKSLIDILSNKLIPNDKSKKQDSLQWFAGVLNKVIINLNKKPNFTISLEKHFNNDKFNNFEFSETKAEKSGLITSQTISTTINNQKYTLVITRDPKQSTFIVESLTKQLVQNN"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GGGTCTCAAA TGAAGAAAAT TCTGGCGATT CTTTCGAGTC
     2041 TGACCTTGGT GTCCACCGGA GTGTTTAGCA CCGTCTTGTC TTGTAAGAAA ACATTAACAC
     2101 CTACAACTAA ACCGAATACA AACAACAATA AGGTTCTTAA GAATAACTCT CTGGATAATA
     2161 TTAAAACGAT CTCGGCAATG TTGTTAAAAC AAGCCGTTTT AGCGGATATG TATGGCTATA
     2221 ACTTTGACTT TCTGAAGTCT TACTTCAATA ATAAAAATCT TAATGAACAA GCTAAGCGTT
     2281 ACAAACTTAA TACCGAAATC AAAGACAATA TTACGCTGTC CACCGACTTT GAGGACGCAC
     2341 TTGCGAATTA CTTCTCAACT AACTTGGTAA TCAAAAAGAA CGACAATGTC AACCTTGACG
     2401 GGATTAAAGG GACGGACATT GACTTCCTGA CATCGGTTTT ACCCAAGACT GTGTTTGGGA
     2461 CTACGAGTAA GCAGATCTCT GCTGCGATTT CCATTATTCT TGAGAACATC TCTGGTGCTG
     2521 GTATCACTGG ACTTCTGGAT CTGGCGAAAA ACATCGACGT GAATAGTAAG TTCTCTGACT
     2581 TCGTTAAGAA TTTAAATGTA TCAAAGGAAC TTATCACTAC GTTACTTAAT ACCATCTTTA
     2641 CTAACGATAA ATTCCTGAAG GAACTTGAGG AGGAAATTAA CAAATTTGAT GCGCTGACGC
     2701 TGTACAAAGA TTTTGAACTT TCGGAGCTGA GCAATCTTGC ACTGTTAAAC ATCTTGGATG
     2761 GCATCAATGG GATCCTTGAC AAAGATTATC AATTGGTATC ATCTGATATT AAGAAGAATA
     2821 ACGGTAGTAC TCTTAACGTG AAATTATGGA ATACTTCAAA AACCTTCATC AATAAGGTTG
     2881 CAAAGTTCGA TCAAACGAGT AATGTAAGCA CGATTTCTAG CTTCTCTAAC TCTACCAGTC
     2941 CAACGATTCT TCCTACAAAC ATCAAACGCA ACATCAAAAC TGCGGCATCA CTGATTCGCG
     3001 GTTTGGAGCT GTTTCAATAC CTGTTTTCGT TATTTGATGA GTCTCGCAAG GACGAGTTCA
     3061 AAATCTCGGA TGAGAATATC TTTGATAAAT CAAAGAAGAA TAGCGAGTTC ATCAAGAATA
     3121 TCTACAAGAT CAATGGGAGC ACCGGGGGGA GCAACAACGG TTCGAATAAG ATTGAAAGCC
     3181 TGAATGGCAC CTCCAACGGT TCTACATCCA AAACTACCCT GAATCTGAAA TATATTATCG
     3241 ACACGCTGCA GTACTACTTG GGTAACTTAG ATAAGAGTGA CAAGGCGTAC CGCCTGCGTC
     3301 AATTTATCGC TATCTTATTT AGCGGAAAGT ATACCGAGAA CATTTACAAG CCCGAAAATA
     3361 ATAACAACGG TAATGGAAGC AACGAGTACA AATCCTTTTT CTTTGAGTTC AACGGTGCAC
     3421 CAGAAAATAA AATTAAAGAA ATTAAGCTTA ACGGCTTTCA GATCTTTCTT ACCTCGATTT
     3481 TATTCGAGTC CTTGAGTAAC ATCAAGTTGC AGAACATTAA GATTGAATCA GGGATTTTCT
     3541 CACTTGCTAA GCCGTTTATC GAAAAGATCA ATCTGAAAAA CTTTTTCGAG TCTGAGGTCT
     3601 TTCTGAAAAA AGGGCTGGCA GATTTCTTGA TCTCCCTTAT GAATTTAATC ACAGACTCGT
     3661 TCGTATACAA TCAACCACTG GTGAACGATA ACTTCGATAA GATCCTGGAG AATCTTGTGA
     3721 CTATCTTAAA AACCCTTAAG TTCGATGACC TTCTGAAGGC ACTTTTTAAT GAAACGAACG
     3781 GGATTGTATC GTCACTGAAG TCCCTGATCG AGAAATACGT CAAGTTTGAA GATATCTCTA
     3841 AGAAGATCGA CGAGTTTATC AAGAAAAAAG AAACCTTCTC TCTGGTGAAG GTGGGGATCA
     3901 AATCCTTTAT CCCCATCCTG GGCGAGAAGT TCTTTGAATA CATTTATGAC GGCAAGGTGG
     3961 AACAGACATT CGATACATTA GCGAATTTAT CAAACGACGT GTTGATTCGC ACACTTGTCG
     4021 AGAAGTTGAA AATCCAAATC CCCGCAGCAT TAAATTTTAT CTTGCCGTAT TTCAAGAAAA
     4081 TTGCCATGTC ATTACGCACT ATTTTTCCGC CGAACGTGCA TCTGAATTTA AAGAATTTGT
     4141 TCACTATCAA GCTGTCCGAC TTCATCAAAC TGGAAAACAA GCCGAATTTT GGTAGCGATT
     4201 ACTTGGACAA AAGTATTACG ACCATCCTTA ATGAACTTAG CGGCGCTGAT GGATCGGGTA
     4261 GTAAGTTGAA GGATCTTGAC AACGCTTATG GATTTAAGAT CGACTCATTG AAAGAATTCA
     4321 TTAATAAAAT CTTCAAGTAC GATTACAAAT GGAACGGCAA GGACCTGGAG AACGGCAATT
     4381 TGATTTCGTT ACTGTTGAAT AACCCTAATA AATTTAAAGA GATCATCGGA CTTACAGAGG
     4441 AAGGAATGAA GAAGGATAGC AAGTCCTTAA TCGATATTTT ATCGAACAAG CTTATCCCGA
     4501 ATGATAAGAG CAAAAAACAA GATTCGCTTC AATGGTTTGC AGGAGTATTG AATAAAGTCA
     4561 TTATCAACCT TAACAAGAAA CCCAACTTCA CCATCAGCTT GGAGAAGCAC TTTAATAATG
     4621 ACAAGTTTAA CAATTTTGAG TTCTCCGAAA CAAAAGCCGA AAAGAGTGGC CTTATTACGA
     4681 GCCAAACCAT CTCTACTACA ATCAATAATC AAAAATATAC TCTGGTAATC ACCCGCGACC
     4741 CCAAACAATC CACTTTTATT GTGGAGTCGT TGACCAAACA GTTGGTCCAG AACAACTGAA
     4801 GAGCTTAGAG ACC
//