LOCUS       Exported     6169 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..6169
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2010..6155
                     /label="MMSYN1_0639"
                     /note="efflux ABC transporter, permease protein"
                     /translation="MKTKNKKNKWLGLILKNSLKNSFKYKSQLFGLVLLVMIMSLIMSLISAINSRVLDKYDDLITNSNQHNLVLKLDPYENVSTSLITSNNQIQAQQQFINRLNEKLYSRYNFKFDWSRTESREFKQVKSLNNLQTLKAVSKQYLTDNKVDQLVIVKGRNINSNKEVLIDPIYAKKHNIKINDIIRFQKDVLGDQLLVNSLENKTTTKQQFEDINKITKQGLTDNNGIYQIKYASSFDWYQVVGFANSADFIFPTINAYSPIPNRLNEGIIYVDPLRFGLIKQTDGFYKYDSTSSKLVVSSNNEWESFYSLKTKQKLSDEIVDWMNQYFSQLINKKAQDKWIYKLEDPNYRFNSRTSVIKKTISAYNIYSFIVLLAVISVVLYTTFLITKKQILNSRGQIGTMRAIGYKKRQMVLNYVMMPFFTSIVGGILGYILSCLISIIIINRFSNYFSLDYGVFSFDWIGLLNNLIFMWLIISSISFLIGYLIMKKGAINLLENRNAKKISKLGSLIKSLSNKRKFNHRLRAALLVNSGSKLTGVGFVVLIATILFTISFVSPNLLKNNKIYAYNGVKYNQIVEYSQPTYNNPFSFIRVFNPDKKSDDKYNIIKNNNRYLATSLPTKNNQYDLQTIINDYLNQTYNNAYYSLAIDLQDKQEVQAINLALSNMKLLQAQDIALTKQYFKYISSLSITPSSIHHILLKNWPDYDNLINKLKEIKENEFETLLNQFKYLQQFYATYTNSIGLAINRSYINSFDLKDKKDLRIQKFNNNSSDQNNLKTKAYDDILNSDLLALSKSSFSAKDFKNKIIDQFKLTNSDSSLGMYHILDNKWNKSNSISDQFLDISAFDFINKKYKLDDLKDLVIKLSLWFSVMFYKRDDQALIQAAYSRAPYFVKQNLKISYNSNKDYTLGFNLTTFNKNYEQLGTLLNVKTLDNKHTFKIYGILNNHDYIDLYDQNKTDLIKKLFDSEQNSIIINQTIAKRLNLKPNDKISLNVLQNELQHIKNNKTTIFKTSDWSMKQDTSYDSFIQRSDISTNNLKVKTNNSVLELNNGFSDVNSYYQSYLNNELKLGTKIQNKTFKIVGIHDGYNENMAWIKESDAQEILNYKQNKSIWWKDIFAPQWNKTFSSIQAKQVLNDTLDLNNKSLTDYSYEQFVNEFINNKNHKNHKIAKKVLQIFDNQFPIFNYKYSKSNDIGNLDTIVSTYSKIADYNPVSLNGQHLENKTSYDGIGQGVIQTITPIQITKQILDQISNLVMLALVLAIITILMIAFVIILLTTSLIISDNTRFIATLKVLGYSNKYITENILGMYFIVIANMLVIGFVSGWFIFDSTIKSLYSIIVLPIIFPIWLPFAVILAVSGIYLITLIVGFNSIYKTDATLTLKDNDV"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /label="ori"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GGGTCTCAAA TGAAAACCAA AAATAAGAAG AATAAATGGC
     2041 TTGGACTTAT CTTAAAGAAC TCACTTAAGA ACTCTTTCAA ATATAAGTCG CAGTTATTTG
     2101 GCCTTGTGTT ATTGGTAATG ATCATGTCTC TTATTATGTC TCTTATCAGT GCTATCAATT
     2161 CTCGTGTTCT TGATAAGTAC GACGATTTGA TTACGAACTC TAACCAACAT AATTTAGTTT
     2221 TAAAATTGGA TCCGTATGAA AACGTTTCAA CGTCATTGAT TACTAGCAAC AACCAGATCC
     2281 AGGCACAACA GCAGTTCATC AATCGTCTGA ATGAGAAATT GTACTCACGC TATAATTTCA
     2341 AATTTGATTG GTCGCGTACT GAATCGCGTG AATTTAAACA GGTGAAGAGT TTAAATAATT
     2401 TGCAAACTCT GAAGGCCGTA AGCAAGCAGT ACCTGACGGA TAATAAGGTT GACCAATTAG
     2461 TAATCGTTAA GGGGCGTAAT ATTAATTCTA ATAAGGAAGT TTTAATCGAC CCGATTTACG
     2521 CGAAGAAACA TAACATTAAG ATCAATGATA TTATCCGTTT CCAAAAGGAT GTATTAGGCG
     2581 ACCAATTGCT GGTGAATTCC CTGGAAAATA AGACCACAAC CAAACAGCAG TTTGAGGACA
     2641 TTAATAAGAT TACTAAACAG GGGTTGACGG ACAATAATGG GATCTATCAA ATTAAGTACG
     2701 CCAGTTCTTT CGATTGGTAC CAGGTTGTTG GATTCGCAAA TAGCGCGGAT TTTATCTTTC
     2761 CTACCATTAA TGCGTATTCG CCCATTCCCA ACCGCCTGAA TGAGGGTATC ATCTACGTTG
     2821 ATCCGTTACG CTTCGGATTA ATTAAGCAAA CCGACGGATT CTATAAATAT GACAGTACGT
     2881 CCAGCAAATT AGTGGTTTCT TCTAACAATG AATGGGAATC GTTCTATAGT CTGAAAACGA
     2941 AGCAAAAGTT AAGTGACGAA ATTGTCGATT GGATGAACCA ATATTTTTCT CAGCTGATTA
     3001 ACAAGAAAGC CCAGGACAAA TGGATCTACA AGTTAGAGGA TCCAAACTAT CGTTTCAACT
     3061 CCCGCACGTC TGTTATTAAA AAGACTATCA GTGCATACAA TATTTATTCA TTCATTGTCT
     3121 TACTTGCAGT CATCTCCGTG GTGCTGTATA CCACATTTTT GATTACTAAA AAGCAAATTT
     3181 TGAATTCTCG CGGTCAGATT GGTACTATGC GTGCCATCGG CTATAAGAAG CGTCAGATGG
     3241 TGCTGAATTA TGTCATGATG CCGTTTTTCA CCTCTATCGT AGGGGGAATT TTAGGCTACA
     3301 TTCTTTCATG TCTTATCAGC ATTATCATTA TCAACCGTTT TTCCAATTAC TTTTCATTGG
     3361 ACTATGGGGT ATTTTCTTTC GACTGGATTG GGTTATTGAA CAACTTAATC TTTATGTGGT
     3421 TGATTATTTC CTCCATCTCG TTTCTTATTG GATACCTGAT TATGAAGAAG GGAGCCATTA
     3481 ATCTGCTGGA AAATCGCAAT GCGAAGAAAA TTTCCAAATT AGGCTCACTT ATTAAGAGTT
     3541 TATCGAATAA GCGTAAATTT AACCACCGCT TGCGCGCAGC GCTGTTGGTG AACTCGGGTT
     3601 CCAAATTAAC TGGTGTAGGA TTCGTTGTTC TGATCGCAAC TATCTTGTTT ACGATTTCAT
     3661 TTGTCAGTCC CAATCTTTTA AAGAACAATA AAATTTACGC CTACAACGGG GTTAAATACA
     3721 ATCAAATCGT GGAATACTCA CAACCAACGT ATAATAACCC CTTTTCATTC ATCCGTGTTT
     3781 TTAATCCTGA CAAAAAGTCC GATGATAAAT ACAATATCAT TAAGAATAAT AACCGTTACT
     3841 TGGCCACGAG CCTGCCGACG AAAAACAACC AATATGATCT GCAGACAATT ATTAATGACT
     3901 ATCTGAATCA GACGTACAAC AATGCTTACT ACAGCCTGGC AATCGATTTA CAGGACAAAC
     3961 AGGAGGTGCA AGCAATCAAT TTGGCTCTGA GCAACATGAA ACTTTTACAA GCGCAAGACA
     4021 TTGCACTGAC CAAACAATAT TTCAAATATA TTTCGAGTTT GTCAATTACA CCCTCTTCAA
     4081 TCCATCACAT CTTGTTAAAA AACTGGCCAG ACTATGATAA CCTGATTAAT AAGTTAAAGG
     4141 AGATTAAGGA AAATGAGTTC GAAACTTTGT TAAATCAGTT TAAGTACCTT CAGCAATTCT
     4201 ATGCGACCTA CACAAACTCC ATCGGACTTG CAATCAATCG TAGTTACATC AACTCCTTCG
     4261 ACTTAAAAGA TAAAAAAGAC CTTCGCATCC AAAAATTTAA TAATAATTCC TCCGACCAAA
     4321 ACAACCTCAA GACAAAGGCG TACGACGATA TCTTAAATTC CGATCTTTTG GCTTTGTCTA
     4381 AATCCTCATT CTCGGCAAAG GACTTTAAGA ATAAGATCAT TGACCAATTC AAGTTAACTA
     4441 ACTCGGATAG CTCTTTAGGT ATGTATCACA TTCTTGATAA CAAGTGGAAT AAGTCAAACA
     4501 GCATTTCGGA CCAGTTCCTT GACATTTCTG CTTTCGACTT TATCAATAAG AAATATAAGC
     4561 TGGACGACTT GAAGGACTTG GTCATTAAGC TTTCATTATG GTTTTCCGTG ATGTTCTACA
     4621 AGCGTGATGA CCAAGCCTTA ATTCAGGCTG CCTATTCGCG CGCGCCGTAT TTCGTTAAAC
     4681 AGAACTTAAA GATCTCATAT AATAGTAATA AGGATTACAC CTTAGGCTTC AACTTGACCA
     4741 CTTTTAACAA GAACTACGAA CAACTTGGTA CTTTACTGAA CGTCAAAACT CTTGACAACA
     4801 AACACACTTT CAAAATCTAC GGCATCCTGA ACAACCACGA TTACATCGAC TTGTATGATC
     4861 AGAACAAGAC CGATTTAATT AAAAAGCTGT TCGACTCAGA ACAGAATTCT ATCATTATTA
     4921 ATCAGACGAT CGCTAAACGC CTTAATCTTA AGCCCAACGA TAAAATCTCC TTAAACGTCT
     4981 TGCAAAATGA GTTACAACAC ATCAAAAACA ACAAGACGAC CATCTTTAAA ACCTCGGACT
     5041 GGTCAATGAA GCAGGACACG AGCTATGATT CCTTTATTCA ACGTTCGGAC ATTTCGACGA
     5101 ATAATCTTAA GGTAAAGACT AACAATTCTG TTCTTGAACT TAACAACGGG TTCAGTGACG
     5161 TGAATTCATA CTATCAAAGT TATTTAAACA ATGAATTGAA GCTTGGTACG AAAATCCAAA
     5221 ACAAAACGTT TAAAATCGTT GGTATCCATG ATGGATACAA CGAAAACATG GCCTGGATTA
     5281 AAGAATCCGA CGCGCAGGAA ATTCTGAATT ACAAGCAGAA TAAGAGCATT TGGTGGAAAG
     5341 ATATTTTCGC TCCCCAGTGG AACAAGACGT TTAGCTCTAT CCAGGCAAAA CAAGTTCTTA
     5401 ATGATACGTT GGACTTGAAT AATAAGTCCC TGACCGACTA TAGTTATGAG CAATTCGTAA
     5461 ATGAGTTTAT CAATAATAAG AACCACAAAA ACCATAAGAT CGCCAAAAAG GTGTTACAGA
     5521 TCTTCGACAA TCAGTTCCCG ATCTTTAACT ATAAATATTC AAAATCCAAT GACATCGGGA
     5581 ATTTAGATAC GATCGTAAGT ACGTACAGCA AAATTGCAGA TTATAACCCT GTTTCGCTTA
     5641 ATGGTCAACA TCTGGAGAAC AAAACGTCAT ACGACGGTAT CGGGCAGGGT GTGATCCAAA
     5701 CGATCACACC GATCCAAATC ACCAAACAGA TCCTGGACCA AATTTCGAAC CTGGTTATGT
     5761 TGGCCCTTGT GTTAGCAATC ATCACCATTC TTATGATCGC ATTTGTAATT ATCTTATTAA
     5821 CCACAAGCTT AATCATTTCG GACAACACTC GTTTTATCGC TACACTGAAG GTCCTTGGCT
     5881 ACTCTAACAA GTATATCACT GAGAATATTC TTGGAATGTA CTTCATCGTG ATTGCCAATA
     5941 TGTTAGTTAT CGGCTTCGTT TCTGGGTGGT TCATCTTCGA TTCTACGATC AAATCGTTAT
     6001 ACTCGATCAT TGTACTGCCT ATCATCTTCC CGATTTGGCT TCCATTTGCC GTTATCTTGG
     6061 CGGTGAGCGG TATTTATTTG ATCACCTTAA TTGTAGGTTT TAACTCGATT TATAAGACCG
     6121 ATGCCACTCT TACCTTAAAG GATAACGATG TGTGAAGAGC TTAGAGACC
//