LOCUS       Exported     2969 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2969
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      982..1029
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2857..2873
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2874..2905
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /label="MMSYN1_0642"
                     /note="ATP-binding (A) component of a common energy-coupling factor (ECF) ABC-transporter complex. Unlike classic ABC transporters this ECF transporter provides the energy necessary to transport a number of different substrates."
                     /translation="MQKVNKKTNQNLKDIDFSKDIILDNVSYTYAKKTPFEFKALNNTSLTFKKNKVTCVIGTTGSGKSTMIQLTNGLIISETGQIIVGDYAIPANTKKIKEVKRLRKEIGLVFQFPEYQLFQETIEKDIAFGPVNLGENKQEAYNKVPELLKLVQLPEDYVKRSPFELSGGQKRRVALAGIIAMDGNTLVLDEPTGGLDPKGEEDFINLFERLNKEYKKRIIMVTHNMDQVLRIADEVIVMHEGKVIAIGSPFEIFSNMELLTKIEIDPPKLYQLMYKLKNKGIDLLNKKIRTIEDFADELAKVLK"
     primer_bind     complement(1034..1050)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1117..1705)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1876..2736)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2737..2841)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /note="ATP-binding (A) component of a common energy-coupling factor (ECF) ABC-transporter complex. Unlike classic ABC transporters this ECF transporter provides the energy necessary to transport a number of different substrates."
                     /translation="MQKVNKKTNQNLKDIDFSKDIILDNVSYTYAKKTPFEFKALNNTSLTFKKNKVTCVIGTTGSGKSTMIQLTNGLIISETGQIIVGDYAIPANTKKIKEVKRLRKEIGLVFQFPEYQLFQETIEKDIAFGPVNLGENKQEAYNKVPELLKLVQLPEDYVKRSPFELSGGQKRRVALAGIIAMDGNTLVLDEPTGGLDPKGEEDFINLFERLNKEYKKRIIMVTHNMDQVLRIADEVIVMHEGKVIAIGSPFEIFSNMELLTKIEIDPPKLYQLMYKLKNKGIDLLNKKIRTIEDFADELAKVLK"
                     /label="MMSYN1_0642"
ORIGIN
        1 ATGCAGAAGG TGAATAAAAA GACAAATCAG AACCTTAAAG ACATAGACTT TTCTAAGGAC
       61 ATTATACTTG ACAATGTGTC TTACACGTAC GCCAAGAAAA CGCCATTCGA ATTCAAGGCG
      121 CTTAATAATA CTTCTTTAAC GTTCAAGAAA AACAAAGTCA CCTGTGTCAT CGGTACAACT
      181 GGATCTGGCA AATCGACTAT GATTCAGCTG ACTAACGGAT TAATCATTTC CGAAACTGGG
      241 CAAATCATAG TCGGTGACTA CGCTATCCCC GCAAATACAA AGAAGATTAA GGAGGTCAAG
      301 CGGCTTCGCA AGGAGATTGG ATTAGTCTTT CAGTTTCCAG AATATCAGTT ATTCCAGGAA
      361 ACGATAGAGA AAGATATCGC CTTCGGCCCG GTGAACTTAG GGGAAAATAA ACAGGAGGCT
      421 TATAATAAAG TTCCCGAGTT ATTGAAGTTG GTACAATTGC CAGAGGACTA CGTTAAGCGT
      481 TCCCCATTCG AGTTGTCTGG TGGGCAGAAA CGTCGTGTTG CATTGGCTGG CATTATCGCC
      541 ATGGACGGCA ACACCTTAGT ATTGGATGAA CCTACGGGAG GACTGGATCC GAAGGGCGAG
      601 GAGGACTTCA TCAACCTTTT CGAGCGGTTG AATAAAGAGT ATAAGAAACG CATCATCATG
      661 GTGACACACA ACATGGATCA AGTGCTGCGG ATCGCTGATG AGGTAATCGT AATGCACGAA
      721 GGAAAAGTTA TTGCTATTGG TTCTCCCTTC GAGATATTTT CCAATATGGA GTTGTTGACA
      781 AAGATCGAAA TTGATCCACC CAAATTATAT CAGTTGATGT ATAAGTTGAA AAATAAGGGA
      841 ATTGATCTTT TAAACAAGAA GATCCGCACT ATCGAGGACT TCGCCGATGA GCTGGCTAAA
      901 GTTTTGAAGT GAAGAGCTTA GAGACCGGTA CTAGTTCCTG CAGGGCTCGA GGTGTCAATC
      961 GTCGGAGCCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG
     1021 GGTTTTTTGC ATGGTCATAG CTGTTTCCTG AGAGCTTGGC AGGTGATGAC ACACATTAAC
     1081 AAATTTCGTG AGGAGTCTCC AGAAGAATGC CATTAATTTC CATAGGCTCC GCCCCCCTGA
     1141 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG
     1201 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT
     1261 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG
     1321 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC
     1381 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT
     1441 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA
     1501 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC
     1561 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC
     1621 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT
     1681 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC
     1741 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT
     1801 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA
     1861 AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT
     1921 ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG
     1981 CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAA CCACGCTCAC CGGCTCCAGA
     2041 TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT
     2101 ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT
     2161 TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT
     2221 TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT
     2281 GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC
     2341 CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC
     2401 CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT
     2461 GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG
     2521 AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT
     2581 ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC
     2641 TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA
     2701 GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG
     2761 AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA
     2821 TAAACAAATA GGGGTTCCGC GCACCTGCAC CAGTCAGTAA AACGACGGCC AGTAGTCAAA
     2881 AGCCTCCGAC CGGAGGCTTT TGACTTGGTC AGGTGGAGTG AATTCGCGGC CGCTTCTAGA
     2941 GGCTCTTCGT AATTACGAAG AGGTCTCTA
//