LOCUS       Exported     3284 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3284
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
                     /label="T7 terminator"
     terminator      1297..1344
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3172..3188
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3189..3220
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /label="MMSYN1_0643"
                     /note="EcfA: Energy-coupling factor transporter, A component"
                     /translation="MDNLAIFEEFNSKKISQDDLEATITSLNNYFVKLNDLNNQYINLIRQDNIDKIEKQNIRQQQKQVKAEIKKISATTKLFKQNLKLAESLYKKIKLTNNQNDINKAKQEVEIAKSMLLQLKEVINGQGKSIKLEKLSDIAIEINHLSFKYGPEFPNAIDDVSFTINQGEYVTIIGHNGSGKSTISKILIGVLNAQHGEIKIFGNIVNDHNIEQARKFLGIVFQNPDNQFIGSTVEADIAFGLENKRIDPKKMPDIILDSAKKVGMEWALKKEPLNLSGGQKQRVAIASTLALDPDIMIFDEATSMLDPKGKREIKEIMVQLRETRTKTILSITHDMDEILNADKVIVLDHGKLVRVAKPLEIVEDKDFLRNIQLDVPFVGLVREELEKKGIKIASTQNIDELVEQICKK"
     primer_bind     complement(1349..1365)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1432..2020)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2191..3051)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3052..3156)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /label="MMSYN1_0643"
                     /note="EcfA: Energy-coupling factor transporter, A component"
                     /translation="MDNLAIFEEFNSKKISQDDLEATITSLNNYFVKLNDLNNQYINLIRQDNIDKIEKQNIRQQQKQVKAEIKKISATTKLFKQNLKLAESLYKKIKLTNNQNDINKAKQEVEIAKSMLLQLKEVINGQGKSIKLEKLSDIAIEINHLSFKYGPEFPNAIDDVSFTINQGEYVTIIGHNGSGKSTISKILIGVLNAQHGEIKIFGNIVNDHNIEQARKFLGIVFQNPDNQFIGSTVEADIAFGLENKRIDPKKMPDIILDSAKKVGMEWALKKEPLNLSGGQKQRVAIASTLALDPDIMIFDEATSMLDPKGKREIKEIMVQLRETRTKTILSITHDMDEILNADKVIVLDHGKLVRVAKPLEIVEDKDFLRNIQLDVPFVGLVREELEKKGIKIASTQNIDELVEQICKK"
ORIGIN
        1 ATGGATAATT TAGCTATTTT CGAAGAGTTC AATTCCAAGA AAATCTCCCA GGATGACTTG
       61 GAAGCGACAA TCACATCTCT GAATAACTAT TTCGTAAAAC TGAACGATTT GAACAACCAG
      121 TATATTAACT TGATCCGCCA AGACAATATC GACAAGATCG AGAAGCAGAA TATTCGCCAG
      181 CAGCAAAAAC AGGTAAAGGC AGAAATTAAG AAAATCTCTG CCACCACCAA GCTGTTCAAG
      241 CAAAACCTTA AGTTGGCCGA ATCATTGTAT AAGAAAATTA AACTGACGAA CAACCAAAAT
      301 GACATCAACA AGGCCAAACA AGAAGTCGAA ATTGCCAAAT CTATGCTTCT GCAGTTGAAA
      361 GAGGTGATCA ACGGTCAAGG AAAATCGATC AAGCTTGAAA AGCTTTCAGA CATTGCGATT
      421 GAGATCAATC ATCTTTCGTT TAAATATGGC CCGGAGTTCC CTAACGCCAT CGATGATGTA
      481 TCATTTACCA TCAACCAGGG CGAATACGTA ACGATTATTG GCCACAATGG TAGTGGTAAA
      541 AGTACGATTA GCAAGATTTT AATCGGAGTT TTGAATGCTC AGCATGGTGA GATCAAGATC
      601 TTTGGAAACA TTGTAAACGA CCATAATATT GAGCAGGCAC GCAAATTCCT TGGGATTGTT
      661 TTCCAGAATC CTGATAATCA GTTCATCGGT TCGACGGTAG AGGCTGATAT TGCTTTTGGT
      721 TTAGAGAACA AACGCATTGA CCCTAAGAAA ATGCCCGACA TTATCTTGGA CTCTGCGAAA
      781 AAGGTAGGTA TGGAATGGGC CCTGAAAAAA GAGCCGTTGA ATCTTTCCGG CGGACAAAAG
      841 CAACGTGTTG CTATCGCCTC TACTCTTGCG CTGGATCCAG ATATCATGAT TTTCGATGAG
      901 GCTACATCGA TGTTAGATCC GAAGGGTAAG CGCGAAATCA AAGAAATCAT GGTCCAACTT
      961 CGTGAAACCC GCACAAAAAC GATTCTGTCG ATCACTCATG ATATGGATGA GATTTTAAAC
     1021 GCCGACAAAG TAATCGTATT GGATCATGGA AAACTTGTGC GCGTGGCTAA GCCGCTGGAA
     1081 ATTGTCGAGG ATAAAGACTT CCTTCGCAAC ATTCAACTGG ATGTTCCCTT CGTTGGTCTG
     1141 GTCCGTGAAG AACTTGAGAA AAAAGGGATT AAAATTGCGT CGACCCAAAA CATTGATGAG
     1201 CTGGTAGAAC AAATTTGCAA GAAGTGAAGA GCTTAGAGAC CGGTACTAGT TCCTGCAGGG
     1261 CTCGAGGTGT CAATCGTCGG AGCCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT
     1321 AAACGGGTCT TGAGGGGTTT TTTGCATGGT CATAGCTGTT TCCTGAGAGC TTGGCAGGTG
     1381 ATGACACACA TTAACAAATT TCGTGAGGAG TCTCCAGAAG AATGCCATTA ATTTCCATAG
     1441 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC
     1501 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT
     1561 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT
     1621 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG
     1681 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT
     1741 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT
     1801 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG
     1861 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA
     1921 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT
     1981 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC
     2041 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT
     2101 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA
     2161 AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT
     2221 CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC
     2281 TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGAACCACG
     2341 CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG
     2401 TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT
     2461 AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT
     2521 GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT
     2581 TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT
     2641 CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT
     2701 TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT
     2761 CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC
     2821 CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA
     2881 ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA
     2941 CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA
     3001 AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT
     3061 TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA
     3121 ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACC TGCACCAGTC AGTAAAACGA
     3181 CGGCCAGTAG TCAAAAGCCT CCGACCGGAG GCTTTTGACT TGGTCAGGTG GAGTGAATTC
     3241 GCGGCCGCTT CTAGAGGCTC TTCGTAATTA CGAAGAGGTC TCTA
//