LOCUS       Exported     2813 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2813
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      826..873
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2701..2717
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 for"
     terminator      2718..2749
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /label="MMSYN1_0650"
                     /note="met_pdase_I: methionine aminopeptidase, type I"
                     /translation="MITIKNQEQIQKMKIAGQVLAKGLNLLKSMIKPGVNCLDLDKAFEEFIKQNGCESNFKNYQGFPKTICISINDQLIHGIPKNRILQNGDIVSIDAGCMYQKWHADSAFTMVCGIANDKKNDILIRVTEKALDLAIAELKPGIRVGTIGSIIQNYVESHNFSVPRDYTGHGIGLALHEDPYIPNYGIPNTGVRLQENMVICIEPMVQMGTYKTKLADDNWTVYSADHSMTAHFEHTILITKDGCEVLTKEER"
     primer_bind     complement(878..894)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(961..1549)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1720..2580)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2581..2685)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /label="MMSYN1_0650"
                     /note="met_pdase_I: methionine aminopeptidase, type I"
                     /translation="MITIKNQEQIQKMKIAGQVLAKGLNLLKSMIKPGVNCLDLDKAFEEFIKQNGCESNFKNYQGFPKTICISINDQLIHGIPKNRILQNGDIVSIDAGCMYQKWHADSAFTMVCGIANDKKNDILIRVTEKALDLAIAELKPGIRVGTIGSIIQNYVESHNFSVPRDYTGHGIGLALHEDPYIPNYGIPNTGVRLQENMVICIEPMVQMGTYKTKLADDNWTVYSADHSMTAHFEHTILITKDGCEVLTKEER"
ORIGIN
        1 ATGATCACGA TCAAGAACCA AGAACAGATT CAAAAAATGA AAATTGCGGG ACAGGTTTTG
       61 GCTAAGGGGT TGAATCTTTT GAAAAGTATG ATCAAACCCG GTGTCAATTG CTTGGACCTG
      121 GACAAAGCAT TCGAGGAGTT CATCAAGCAA AATGGCTGTG AATCCAACTT TAAGAACTAC
      181 CAAGGATTCC CAAAAACGAT TTGCATCAGT ATCAATGATC AGCTGATCCA TGGGATCCCC
      241 AAAAATCGTA TCTTACAAAA TGGAGATATT GTCTCTATTG ACGCCGGCTG CATGTACCAG
      301 AAGTGGCACG CGGATTCCGC TTTCACCATG GTATGCGGGA TTGCAAATGA TAAGAAAAAT
      361 GACATTCTTA TTCGCGTGAC TGAAAAGGCG TTGGATCTGG CAATTGCAGA ATTGAAGCCT
      421 GGCATCCGTG TTGGAACGAT CGGGTCGATC ATTCAGAATT ATGTTGAATC ACATAACTTT
      481 TCAGTTCCCC GCGATTACAC AGGCCATGGC ATCGGATTGG CGTTACACGA GGATCCCTAC
      541 ATTCCAAACT ATGGCATTCC AAATACGGGG GTTCGTTTAC AGGAGAATAT GGTCATTTGT
      601 ATCGAGCCAA TGGTGCAGAT GGGTACTTAT AAAACAAAAT TGGCTGACGA TAATTGGACA
      661 GTATATAGTG CCGACCACAG CATGACCGCT CACTTTGAAC ATACAATTCT GATTACCAAG
      721 GACGGTTGCG AGGTCTTAAC GAAGGAGGAA CGCTGAAGAG CTTAGAGACC GGTACTAGTT
      781 CCTGCAGGGC TCGAGGTGTC AATCGTCGGA GCCGCTGAGC AATAACTAGC ATAACCCCTT
      841 GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCATGGTC ATAGCTGTTT CCTGAGAGCT
      901 TGGCAGGTGA TGACACACAT TAACAAATTT CGTGAGGAGT CTCCAGAAGA ATGCCATTAA
      961 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG
     1021 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG
     1081 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG
     1141 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC
     1201 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA
     1261 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG
     1321 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC
     1381 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC
     1441 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG
     1501 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT
     1561 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT
     1621 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA
     1681 ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA
     1741 GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT
     1801 GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG
     1861 AGAACCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA
     1921 GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA
     1981 AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG
     2041 CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC
     2101 AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC
     2161 GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA
     2221 TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC
     2281 CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG
     2341 GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC
     2401 GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG
     2461 TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC
     2521 AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT
     2581 ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA
     2641 CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACCT GCACCAGTCA
     2701 GTAAAACGAC GGCCAGTAGT CAAAAGCCTC CGACCGGAGG CTTTTGACTT GGTCAGGTGG
     2761 AGTGAATTCG CGGCCGCTTC TAGAGGCTCT TCGTAATTAC GAAGAGGTCT CTA
//