LOCUS       Exported     3506 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3506
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
                     /label="T7 terminator"
     terminator      1519..1566
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3394..3410
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3411..3442
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /translation="MVIKKPANKVDKKTTFKSSTKKKNLFKSNFFTKNKDLILRILFTLLALIIIRLGVYITVPGVTLDKRFATDSSRIQFFQLLSTLGGGSIGRFSILALGVSPYITASIIVQLLSTDVIPVLTRWSKSGERGRKKLDKLTKIIMIPFALMQAEATIFTLSSQGLIVPGWDSTNVIANSAFYYVLIPLVMLGGSFFMLWIADQITIKGIGNGISIVIFIGIIISMPTNLKATFEYWVSNSGEEANIFFSGLLNFMIYISVFLLVILSVVIMNEAERKIPIQQTGSGLTDSSEHTPYLPLKLNNAGVIPVIFASAIISTPITISQIIEAVNPDSGFVIFTRDYLSFNTWWGISIFGILIVLFTFLYSQVQINPEKVAENFQKSGTFIPGIKPGKDTTKYLTGIINRLSVVGSVFLAIIALLPYVISKLTQLPSNLAIGGTGLIICISVAIQTVQQLKGRIIQQNFIEKKKEKFTNNINKNKTSHIW"
                     /label="MMSYN1_0652"
                     /note="The central subunit of the protein translocation channel SecYEG. Consists of two halves formed by TMs 1-5 and 6-10. These two domains form a lateral gate at the front which open onto the bilayer between TMs 2 and 7, and are clamped together by SecE at the back. The channel is closed by both a pore ring composed of hydrophobic SecY resides and a short helix (helix 2A) on the extracellular side of the membrane which forms a plug. The plug probably moves laterally to allow the channel to open. The ring and the pore may move independently."
     primer_bind     complement(1571..1587)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1654..2242)
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /label="ori"
     CDS             complement(2413..3273)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3274..3378)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /label="MMSYN1_0652"
                     /note="The central subunit of the protein translocation channel SecYEG. Consists of two halves formed by TMs 1-5 and 6-10. These two domains form a lateral gate at the front which open onto the bilayer between TMs 2 and 7, and are clamped together by SecE at the back. The channel is closed by both a pore ring composed of hydrophobic SecY resides and a short helix (helix 2A) on the extracellular side of the membrane which forms a plug. The plug probably moves laterally to allow the channel to open. The ring and the pore may move independently."
                     /translation="MVIKKPANKVDKKTTFKSSTKKKNLFKSNFFTKNKDLILRILFTLLALIIIRLGVYITVPGVTLDKRFATDSSRIQFFQLLSTLGGGSIGRFSILALGVSPYITASIIVQLLSTDVIPVLTRWSKSGERGRKKLDKLTKIIMIPFALMQAEATIFTLSSQGLIVPGWDSTNVIANSAFYYVLIPLVMLGGSFFMLWIADQITIKGIGNGISIVIFIGIIISMPTNLKATFEYWVSNSGEEANIFFSGLLNFMIYISVFLLVILSVVIMNEAERKIPIQQTGSGLTDSSEHTPYLPLKLNNAGVIPVIFASAIISTPITISQIIEAVNPDSGFVIFTRDYLSFNTWWGISIFGILIVLFTFLYSQVQINPEKVAENFQKSGTFIPGIKPGKDTTKYLTGIINRLSVVGSVFLAIIALLPYVISKLTQLPSNLAIGGTGLIICISVAIQTVQQLKGRIIQQNFIEKKKEKFTNNINKNKTSHIW"
ORIGIN
        1 ATGGTTATTA AAAAGCCGGC CAACAAGGTG GACAAGAAAA CAACCTTTAA ATCATCCACG
       61 AAAAAGAAGA ATCTGTTCAA ATCTAACTTC TTCACGAAAA ATAAAGATCT GATCCTTCGT
      121 ATCCTTTTCA CATTACTGGC TTTGATTATC ATTCGCCTGG GGGTCTATAT CACTGTACCC
      181 GGAGTAACAC TGGACAAGCG TTTTGCAACA GATAGTAGTC GCATCCAGTT TTTCCAGCTG
      241 CTTTCCACTT TAGGGGGCGG ATCGATTGGC CGCTTCTCTA TCCTTGCTCT TGGTGTTAGT
      301 CCTTATATCA CCGCTTCAAT CATCGTGCAG CTGCTGTCGA CAGATGTCAT TCCAGTTTTA
      361 ACGCGTTGGA GTAAAAGTGG GGAGCGCGGC CGCAAGAAAC TTGACAAGCT TACGAAAATT
      421 ATTATGATTC CCTTTGCCTT AATGCAAGCG GAGGCCACCA TTTTTACTCT TTCATCTCAG
      481 GGATTAATTG TACCCGGCTG GGATAGCACG AATGTCATTG CGAATAGTGC CTTTTACTAT
      541 GTGCTTATCC CGTTAGTGAT GCTGGGAGGC TCTTTCTTCA TGTTGTGGAT TGCCGATCAA
      601 ATCACCATCA AGGGTATTGG CAACGGCATC TCGATTGTAA TCTTTATCGG AATTATCATC
      661 TCAATGCCAA CAAATCTGAA GGCTACGTTC GAATATTGGG TCAGTAATTC GGGCGAGGAA
      721 GCGAATATCT TCTTCTCAGG GCTGCTGAAC TTTATGATCT ACATCTCTGT TTTCCTGTTA
      781 GTGATTCTTT CAGTCGTGAT TATGAACGAA GCCGAGCGTA AAATTCCAAT TCAACAGACC
      841 GGCAGTGGGC TGACGGATTC ATCTGAACAC ACTCCTTACT TACCGCTTAA GTTAAACAAT
      901 GCCGGGGTGA TCCCAGTAAT TTTTGCGTCT GCAATCATCT CCACCCCAAT TACTATTTCA
      961 CAAATTATCG AAGCAGTGAA TCCTGATAGT GGGTTCGTCA TCTTCACGCG CGACTACTTA
     1021 TCTTTTAATA CGTGGTGGGG GATTAGCATC TTTGGCATTC TTATCGTGCT TTTCACGTTC
     1081 TTATACAGCC AAGTACAGAT CAACCCGGAG AAAGTCGCGG AGAATTTCCA AAAGAGCGGT
     1141 ACGTTCATTC CAGGCATCAA ACCAGGAAAG GACACTACGA AGTATCTTAC AGGCATTATC
     1201 AACCGTCTTT CAGTTGTAGG CTCTGTGTTT TTAGCCATTA TCGCCTTGTT GCCTTATGTT
     1261 ATCTCGAAAT TAACCCAACT GCCGAGTAAT TTGGCAATTG GCGGTACGGG ACTTATTATT
     1321 TGTATCTCAG TTGCCATTCA AACTGTCCAG CAGTTAAAGG GCCGCATCAT TCAACAGAAT
     1381 TTCATCGAGA AAAAGAAGGA AAAGTTCACA AACAATATTA ACAAGAACAA GACATCCCAC
     1441 ATCTGGTGAA GAGCTTAGAG ACCGGTACTA GTTCCTGCAG GGCTCGAGGT GTCAATCGTC
     1501 GGAGCCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT
     1561 TTTTTGCATG GTCATAGCTG TTTCCTGAGA GCTTGGCAGG TGATGACACA CATTAACAAA
     1621 TTTCGTGAGG AGTCTCCAGA AGAATGCCAT TAATTTCCAT AGGCTCCGCC CCCCTGACGA
     1681 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA
     1741 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC
     1801 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG
     1861 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC
     1921 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG
     1981 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT
     2041 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT
     2101 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG
     2161 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC
     2221 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA
     2281 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC
     2341 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC
     2401 TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT
     2461 TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT
     2521 ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGAACCA CGCTCACCGG CTCCAGATTT
     2581 ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC
     2641 CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA
     2701 TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG
     2761 TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT
     2821 GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC
     2881 AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT
     2941 AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG
     3001 GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC
     3061 TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC
     3121 GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT
     3181 TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG
     3241 AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG
     3301 CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA
     3361 ACAAATAGGG GTTCCGCGCA CCTGCACCAG TCAGTAAAAC GACGGCCAGT AGTCAAAAGC
     3421 CTCCGACCGG AGGCTTTTGA CTTGGTCAGG TGGAGTGAAT TCGCGGCCGC TTCTAGAGGC
     3481 TCTTCGTAAT TACGAAGAGG TCTCTA
//