LOCUS       Exported     2822 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2822
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      835..882
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2710..2726
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2727..2758
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /label="MMSYN1_0654"
                     /note="With S4 and S12 plays an important role in translational accuracy."
                     /translation="MTEEMNVVETSSEMNSNVEKASTQVKETKKFERRTRPQSKSKQVKDEFEEKVVTIRRVTKVTKGGRHFRFAAVVVVGNKKGLVGMGTGKANEVPEAIKKAIKEAKKNLVSVTLRNTTVPHEVLGTFGAGKILIKPAKVGTGIIAGGPARAVIELAGISDVYAKSLGSNNAINMIRATFEGLSSMQTLKRVQELRYGKTFDTQKVKPVEQKVAEVKSVEKKQPKQVVKKVTVKKAENQENTVEVITNAETESKAE"
     primer_bind     complement(887..903)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(970..1558)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1729..2589)
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
     promoter        complement(2590..2694)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /label="MMSYN1_0654"
                     /note="With S4 and S12 plays an important role in translational accuracy."
                     /translation="MTEEMNVVETSSEMNSNVEKASTQVKETKKFERRTRPQSKSKQVKDEFEEKVVTIRRVTKVTKGGRHFRFAAVVVVGNKKGLVGMGTGKANEVPEAIKKAIKEAKKNLVSVTLRNTTVPHEVLGTFGAGKILIKPAKVGTGIIAGGPARAVIELAGISDVYAKSLGSNNAINMIRATFEGLSSMQTLKRVQELRYGKTFDTQKVKPVEQKVAEVKSVEKKQPKQVVKKVTVKKAENQENTVEVITNAETESKAE"
ORIGIN
        1 ATGACTGAAG AGATGAATGT TGTAGAAACC AGCTCTGAGA TGAACTCAAA CGTAGAGAAA
       61 GCCTCTACCC AGGTCAAGGA AACTAAAAAG TTCGAGCGTC GTACCCGTCC GCAGTCCAAG
      121 AGCAAACAAG TAAAAGACGA GTTTGAAGAG AAGGTGGTAA CTATCCGCCG CGTGACAAAG
      181 GTTACAAAGG GTGGTCGCCA CTTTCGCTTT GCCGCGGTGG TTGTTGTTGG GAATAAAAAG
      241 GGTCTGGTTG GCATGGGAAC AGGCAAAGCA AATGAGGTGC CGGAAGCCAT TAAAAAAGCA
      301 ATTAAAGAGG CTAAGAAGAA CTTAGTTTCA GTCACGCTTC GCAATACCAC GGTCCCTCAC
      361 GAAGTCCTGG GGACGTTTGG GGCTGGTAAA ATTTTGATTA AACCAGCTAA AGTCGGAACG
      421 GGAATTATCG CAGGCGGTCC GGCGCGCGCT GTAATTGAGC TTGCCGGAAT TTCAGATGTG
      481 TACGCGAAAT CGTTAGGGTC GAACAACGCA ATCAATATGA TTCGCGCTAC TTTTGAGGGC
      541 TTATCCAGTA TGCAGACTTT GAAACGCGTG CAAGAACTGC GCTACGGTAA AACTTTTGAT
      601 ACCCAAAAGG TCAAACCTGT TGAGCAAAAA GTAGCTGAAG TTAAGTCCGT TGAGAAAAAG
      661 CAGCCCAAAC AGGTAGTAAA AAAGGTTACT GTCAAAAAGG CAGAGAATCA GGAGAACACA
      721 GTTGAGGTAA TCACGAATGC AGAGACTGAG TCCAAAGCCG AGTGAAGAGC TTAGAGACCG
      781 GTACTAGTTC CTGCAGGGCT CGAGGTGTCA ATCGTCGGAG CCGCTGAGCA ATAACTAGCA
      841 TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCATGGTCA TAGCTGTTTC
      901 CTGAGAGCTT GGCAGGTGAT GACACACATT AACAAATTTC GTGAGGAGTC TCCAGAAGAA
      961 TGCCATTAAT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG
     1021 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC
     1081 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC
     1141 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT
     1201 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT
     1261 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC
     1321 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA
     1381 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA
     1441 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG
     1501 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA
     1561 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG
     1621 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG
     1681 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT
     1741 AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT
     1801 CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT
     1861 GATACCGCGA GAACCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG
     1921 AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG
     1981 TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT
     2041 TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC
     2101 CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT
     2161 CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC
     2221 AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA
     2281 GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC
     2341 GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA
     2401 ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA
     2461 ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG
     2521 AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG
     2581 AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT
     2641 GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACCTG
     2701 CACCAGTCAG TAAAACGACG GCCAGTAGTC AAAAGCCTCC GACCGGAGGC TTTTGACTTG
     2761 GTCAGGTGGA GTGAATTCGC GGCCGCTTCT AGAGGCTCTT CGTAATTACG AAGAGGTCTC
     2821 TA
//