LOCUS       Exported     2759 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2759
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      772..819
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2647..2663
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2664..2695
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /label="MMSYN1_0665"
                     /note="Binds the lower part of the 30S subunit head. Binds mRNA in the 70S ribosome, positioning it for translation."
                     /translation="MGQKVSPNVLRLGIVRDWENRWYAEKDQYVKWLDQDIKIRTALFKLLKDAAVSKIDIERTTKDLTLFIKTARPAIVLGQEGKNIEKIVLAVRKTVKNKKLIVNVRVIEIKSPDADATLVARWIGEQISNRASFRTVQKLAIKKALKAGAKGIKTAVSGRLGGVEMARTEGYLEGSVPLSTLRNNIDYALYEAPTTYGQIGVKVWINHGEVFKKERMNNSQIMAKPRTNKGGKR"
     primer_bind     complement(824..840)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(907..1495)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1666..2526)
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
     promoter        complement(2527..2631)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /label="MMSYN1_0665"
                     /note="Binds the lower part of the 30S subunit head. Binds mRNA in the 70S ribosome, positioning it for translation."
                     /translation="MGQKVSPNVLRLGIVRDWENRWYAEKDQYVKWLDQDIKIRTALFKLLKDAAVSKIDIERTTKDLTLFIKTARPAIVLGQEGKNIEKIVLAVRKTVKNKKLIVNVRVIEIKSPDADATLVARWIGEQISNRASFRTVQKLAIKKALKAGAKGIKTAVSGRLGGVEMARTEGYLEGSVPLSTLRNNIDYALYEAPTTYGQIGVKVWINHGEVFKKERMNNSQIMAKPRTNKGGKR"
ORIGIN
        1 ATGGGCCAAA AGGTTAGCCC TAATGTCTTA CGTTTGGGGA TTGTACGTGA CTGGGAAAAT
       61 CGTTGGTATG CAGAGAAAGA CCAGTATGTG AAGTGGCTTG ACCAAGACAT CAAAATCCGC
      121 ACTGCCTTAT TCAAATTACT TAAAGACGCT GCAGTATCGA AAATCGACAT TGAACGCACG
      181 ACCAAAGATT TAACATTATT CATTAAGACC GCGCGTCCTG CAATCGTCCT TGGTCAAGAA
      241 GGCAAAAATA TCGAAAAGAT TGTCCTTGCG GTCCGCAAAA CGGTTAAAAA TAAGAAGCTT
      301 ATCGTCAATG TCCGTGTGAT CGAGATTAAA TCTCCAGACG CTGATGCAAC TCTTGTGGCT
      361 CGTTGGATTG GAGAACAAAT TTCCAACCGT GCTTCCTTCC GTACGGTTCA AAAGCTTGCC
      421 ATTAAGAAAG CATTAAAGGC CGGTGCGAAG GGAATCAAGA CAGCTGTATC AGGTCGCCTT
      481 GGAGGAGTTG AGATGGCTCG CACCGAGGGG TATCTTGAAG GGTCTGTGCC ACTTTCGACG
      541 TTACGCAATA ATATCGACTA TGCACTTTAT GAAGCCCCCA CAACTTACGG TCAAATTGGA
      601 GTAAAGGTCT GGATCAATCA CGGCGAAGTA TTTAAGAAAG AGCGTATGAA TAATTCCCAA
      661 ATCATGGCGA AACCCCGTAC AAACAAAGGC GGAAAGCGCT GAAGAGCTTA GAGACCGGTA
      721 CTAGTTCCTG CAGGGCTCGA GGTGTCAATC GTCGGAGCCG CTGAGCAATA ACTAGCATAA
      781 CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC ATGGTCATAG CTGTTTCCTG
      841 AGAGCTTGGC AGGTGATGAC ACACATTAAC AAATTTCGTG AGGAGTCTCC AGAAGAATGC
      901 CATTAATTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA
      961 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT
     1021 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC
     1081 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT
     1141 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC
     1201 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC
     1261 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG
     1321 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC
     1381 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG
     1441 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA
     1501 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT
     1561 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG
     1621 TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT
     1681 CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC
     1741 CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT
     1801 ACCGCGAGAA CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG
     1861 GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG
     1921 CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC
     1981 TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA
     2041 ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG
     2101 TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC
     2161 ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA
     2221 CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC
     2281 AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG
     2341 TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC
     2401 CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC
     2461 AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT
     2521 ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG
     2581 CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACCTGCAC
     2641 CAGTCAGTAA AACGACGGCC AGTAGTCAAA AGCCTCCGAC CGGAGGCTTT TGACTTGGTC
     2701 AGGTGGAGTG AATTCGCGGC CGCTTCTAGA GGCTCTTCGT AATTACGAAG AGGTCTCTA
//