LOCUS       Exported     2729 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2729
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      742..789
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2617..2633
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2634..2665
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /label="MMSYN1_0671"
                     /note="One of the primary rRNA binding proteins, it binds directly near the 3'-end of the 23S rRNA, where it nucleates assembly of the 50S subunit."
                     /translation="MKGILGRKVEMTQVFTSAGQLVPVTVVEVLPNTVLQVKTIDSDGYVAVQLGTTDKRVNLVNKPELGHFKKANSNPKRFVKEIRNMQGYELGQVINVSDIFVSGEYVDVTGISKGKGFAGGIKRHNYARGPMAHGSGYHRGIGSMGAIINRIFKSKKMPGHMGNAKRTIQNLEIIAIDQPNNIMLIKGSIPGPKNSFVQIKQNIKGMSSKQAVELLNRNASVEA"
     primer_bind     complement(794..810)
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 rev"
     rep_origin      complement(877..1465)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1636..2496)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2497..2601)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /label="MMSYN1_0671"
                     /note="One of the primary rRNA binding proteins, it binds directly near the 3'-end of the 23S rRNA, where it nucleates assembly of the 50S subunit."
                     /translation="MKGILGRKVEMTQVFTSAGQLVPVTVVEVLPNTVLQVKTIDSDGYVAVQLGTTDKRVNLVNKPELGHFKKANSNPKRFVKEIRNMQGYELGQVINVSDIFVSGEYVDVTGISKGKGFAGGIKRHNYARGPMAHGSGYHRGIGSMGAIINRIFKSKKMPGHMGNAKRTIQNLEIIAIDQPNNIMLIKGSIPGPKNSFVQIKQNIKGMSSKQAVELLNRNASVEA"
ORIGIN
        1 ATGAAAGGAA TCTTAGGTCG TAAGGTAGAA ATGACTCAAG TATTTACTAG TGCTGGTCAA
       61 TTAGTTCCAG TAACAGTAGT TGAAGTTCTA CCAAACACAG TTTTACAAGT TAAAACTATT
      121 GACAGTGATG GATATGTTGC TGTGCAATTA GGTACTACAG ATAAAAGAGT TAACTTAGTA
      181 AATAAACCTG AATTAGGACA CTTTAAGAAA GCTAATTCAA ATCCTAAGCG CTTCGTAAAA
      241 GAAATCAGAA ACATGCAAGG ATATGAACTT GGACAAGTTA TTAATGTAAG TGATATCTTT
      301 GTTTCTGGTG AATACGTTGA TGTTACAGGA ATTTCTAAAG GTAAAGGTTT TGCTGGAGGA
      361 ATTAAAAGAC ATAATTATGC AAGAGGACCA ATGGCTCATG GATCAGGATA TCATAGAGGA
      421 ATTGGTTCAA TGGGAGCAAT CATTAACCGT ATTTTTAAAA GCAAGAAAAT GCCAGGGCAC
      481 ATGGGTAATG CAAAAAGAAC TATTCAAAAT CTAGAAATTA TTGCTATTGA TCAACCAAAC
      541 AACATTATGT TAATCAAAGG ATCAATTCCT GGACCAAAAA ATAGTTTTGT ACAAATCAAA
      601 CAAAATATTA AAGGTATGAG TTCTAAACAA GCAGTTGAAT TATTAAATAG AAATGCATCA
      661 GTTGAAGCCT GAAGAGCTTA GAGACCGGTA CTAGTTCCTG CAGGGCTCGA GGTGTCAATC
      721 GTCGGAGCCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG
      781 GGTTTTTTGC ATGGTCATAG CTGTTTCCTG AGAGCTTGGC AGGTGATGAC ACACATTAAC
      841 AAATTTCGTG AGGAGTCTCC AGAAGAATGC CATTAATTTC CATAGGCTCC GCCCCCCTGA
      901 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG
      961 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT
     1021 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG
     1081 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC
     1141 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT
     1201 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA
     1261 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC
     1321 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC
     1381 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT
     1441 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC
     1501 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT
     1561 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA
     1621 AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT
     1681 ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG
     1741 CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAA CCACGCTCAC CGGCTCCAGA
     1801 TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT
     1861 ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT
     1921 TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT
     1981 TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT
     2041 GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC
     2101 CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC
     2161 CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT
     2221 GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG
     2281 AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT
     2341 ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC
     2401 TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA
     2461 GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG
     2521 AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA
     2581 TAAACAAATA GGGGTTCCGC GCACCTGCAC CAGTCAGTAA AACGACGGCC AGTAGTCAAA
     2641 AGCCTCCGAC CGGAGGCTTT TGACTTGGTC AGGTGGAGTG AATTCGCGGC CGCTTCTAGA
     2701 GGCTCTTCGT AATTACGAAG AGGTCTCTA
//