LOCUS       Exported     2924 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2924
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      937..984
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
                     /label="T7 terminator"
     primer_bind     2812..2828
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 for"
     terminator      2829..2860
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /label="MMSYN1_0684"
                     /note="Catalyzes the oxidation of 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10-formyltetrahydrofolate."
                     /translation="MVILDGKLVSKQIKETLKQQIDTYLNKNYKKPKLVVILIGNDPASELYVSNKIKACNLVGIESVLLRFDQNITSEILSDQINQLNNDNSVDAILLQLPLPKHLNEQEFLQAIDPLKDVDGFHYINQGKMLEGYDTIYPCTPIGIINLLKAYNIDVRSKDITIIGTSNIVGKPLAIMLSNMGATISMCNKNTKSLKKYTKRSDIVISATGKQALIKKDMIKKNAIVIDVGIIKDPITNKIVGDVDFENVKELCSYISPVPGGVGPMTVAMLLENTFELYKLHIKENYEN"
     primer_bind     complement(989..1005)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1072..1660)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1831..2691)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2692..2796)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /label="MMSYN1_0684"
                     /note="Catalyzes the oxidation of 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10-formyltetrahydrofolate."
                     /translation="MVILDGKLVSKQIKETLKQQIDTYLNKNYKKPKLVVILIGNDPASELYVSNKIKACNLVGIESVLLRFDQNITSEILSDQINQLNNDNSVDAILLQLPLPKHLNEQEFLQAIDPLKDVDGFHYINQGKMLEGYDTIYPCTPIGIINLLKAYNIDVRSKDITIIGTSNIVGKPLAIMLSNMGATISMCNKNTKSLKKYTKRSDIVISATGKQALIKKDMIKKNAIVIDVGIIKDPITNKIVGDVDFENVKELCSYISPVPGGVGPMTVAMLLENTFELYKLHIKENYEN"
ORIGIN
        1 ATGGTAATCT TGGATGGTAA GCTTGTTAGT AAGCAGATCA AGGAAACCTT GAAGCAGCAA
       61 ATCGACACGT ATCTTAATAA AAACTACAAG AAACCTAAGC TGGTCGTAAT TTTAATCGGA
      121 AATGATCCTG CAAGCGAGCT TTACGTATCA AATAAGATTA AGGCCTGTAA CCTTGTAGGC
      181 ATCGAATCCG TTTTATTGCG TTTCGATCAA AACATTACGA GTGAGATCCT GTCCGACCAG
      241 ATCAACCAGT TGAACAATGA CAATTCTGTA GATGCCATCT TATTGCAATT ACCGCTGCCT
      301 AAGCACCTTA ACGAACAGGA ATTCCTGCAA GCAATCGACC CATTAAAGGA CGTTGATGGG
      361 TTCCATTATA TTAACCAAGG AAAAATGCTG GAAGGCTACG ATACGATCTA CCCGTGCACA
      421 CCTATCGGTA TCATTAATTT GCTGAAAGCT TACAATATCG ATGTACGTTC CAAGGACATC
      481 ACAATCATCG GGACTTCAAA TATTGTGGGT AAACCCTTAG CCATTATGTT GAGCAATATG
      541 GGCGCAACCA TTAGTATGTG CAATAAGAAT ACCAAATCGT TGAAAAAATA TACGAAACGC
      601 TCGGATATCG TAATCTCTGC AACTGGAAAA CAGGCTCTTA TTAAAAAAGA CATGATTAAG
      661 AAAAATGCTA TCGTGATCGA CGTCGGCATC ATCAAAGACC CCATTACCAA TAAAATTGTA
      721 GGGGACGTAG ACTTCGAAAA CGTAAAAGAG TTGTGCAGCT ATATCTCGCC TGTTCCAGGG
      781 GGCGTAGGTC CCATGACAGT GGCAATGTTA TTGGAGAACA CTTTTGAACT GTATAAGCTT
      841 CACATCAAGG AAAACTATGA AAACTGAAGA GCTTAGAGAC CGGTACTAGT TCCTGCAGGG
      901 CTCGAGGTGT CAATCGTCGG AGCCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT
      961 AAACGGGTCT TGAGGGGTTT TTTGCATGGT CATAGCTGTT TCCTGAGAGC TTGGCAGGTG
     1021 ATGACACACA TTAACAAATT TCGTGAGGAG TCTCCAGAAG AATGCCATTA ATTTCCATAG
     1081 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC
     1141 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT
     1201 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT
     1261 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG
     1321 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT
     1381 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT
     1441 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG
     1501 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA
     1561 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT
     1621 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC
     1681 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT
     1741 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA
     1801 AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT
     1861 CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC
     1921 TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGAACCACG
     1981 CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG
     2041 TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT
     2101 AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT
     2161 GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT
     2221 TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT
     2281 CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT
     2341 TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT
     2401 CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC
     2461 CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA
     2521 ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA
     2581 CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA
     2641 AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT
     2701 TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA
     2761 ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACC TGCACCAGTC AGTAAAACGA
     2821 CGGCCAGTAG TCAAAAGCCT CCGACCGGAG GCTTTTGACT TGGTCAGGTG GAGTGAATTC
     2881 GCGGCCGCTT CTAGAGGCTC TTCGTAATTA CGAAGAGGTC TCTA
//