LOCUS       Exported     3659 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3659
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1672..1719
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3547..3563
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3564..3595
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /label="MMSYN1_0685"
                     /note=""
                     /translation="MFFKKKNNKKHKLKFTSRLFKKTSNFNEEKYKFFRLVKDIWPLSKTSEKIFLIYVAIILLGGLLLSIPNFSLTKSGSKYNWDFLTGIFIASSGFSDTGLTVLDVSHSYTFWGQLILLLLIEFGGIGVLTFKIVLFLIINKKISISDTIVAQSERGSATTSLTIDLIKDGFIWLTSVQVISAFILFFLFFFNQPSNNPNLEVVSPYHDFWKSLWFAVFHSTSAVNNAGFDIISPNSLQPYNVDNHRVYAIQVIFMLEWIIGGLGYPTFHDIKRKLKARKTKEKINFSLFTKINFWVYLVLFIFGPLAVFATEYSNYNNSLIFHYYDENFTVLNAKSNTVVFMDILFNTTASRNAGFSTIDISTFNSGSKAILSILMFIGSAPSSTAGGIRTTTFGVLLLSTFTIIKNQKFTSAFRKTIPSETVNRSYAAFFISTFLIFIALFIIYVDSNSVFHTLKNHNSASINTILLITSAFGTVGLSPLAHFQMYQLGVVTKISLILIMFIGQLGVSNTLLIFLKPARDKAYKYLEEDITIG"
     primer_bind     complement(1724..1740)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1807..2395)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2566..3426)
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
     promoter        complement(3427..3531)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /label="MMSYN1_0685"
                     /note=""
                     /translation="MFFKKKNNKKHKLKFTSRLFKKTSNFNEEKYKFFRLVKDIWPLSKTSEKIFLIYVAIILLGGLLLSIPNFSLTKSGSKYNWDFLTGIFIASSGFSDTGLTVLDVSHSYTFWGQLILLLLIEFGGIGVLTFKIVLFLIINKKISISDTIVAQSERGSATTSLTIDLIKDGFIWLTSVQVISAFILFFLFFFNQPSNNPNLEVVSPYHDFWKSLWFAVFHSTSAVNNAGFDIISPNSLQPYNVDNHRVYAIQVIFMLEWIIGGLGYPTFHDIKRKLKARKTKEKINFSLFTKINFWVYLVLFIFGPLAVFATEYSNYNNSLIFHYYDENFTVLNAKSNTVVFMDILFNTTASRNAGFSTIDISTFNSGSKAILSILMFIGSAPSSTAGGIRTTTFGVLLLSTFTIIKNQKFTSAFRKTIPSETVNRSYAAFFISTFLIFIALFIIYVDSNSVFHTLKNHNSASINTILLITSAFGTVGLSPLAHFQMYQLGVVTKISLILIMFIGQLGVSNTLLIFLKPARDKAYKYLEEDITIG"
ORIGIN
        1 ATGTTTTTCA AGAAGAAGAA CAACAAGAAG CACAAGTTAA AATTCACCTC TCGTCTGTTC
       61 AAGAAAACTT CAAATTTTAA TGAGGAGAAA TACAAATTCT TCAGATTAGT CAAAGACATC
      121 TGGCCTTTAA GCAAAACCTC CGAAAAAATC TTTTTAATTT ATGTCGCAAT AATTTTGCTT
      181 GGAGGTCTGT TACTGTCAAT ACCCAACTTT TCACTGACCA AGTCGGGATC CAAGTATAAC
      241 TGGGATTTTC TTACAGGCAT TTTCATAGCG TCCAGTGGCT TTAGCGACAC AGGGTTGACT
      301 GTGTTGGACG TATCGCACAG CTACACATTC TGGGGTCAAC TGATTTTATT GTTATTGATA
      361 GAATTTGGGG GTATAGGGGT CTTGACTTTT AAAATAGTCC TTTTTCTTAT CATCAACAAG
      421 AAAATTTCAA TTAGCGATAC TATTGTTGCG CAATCGGAGC GTGGCTCAGC TACTACCAGC
      481 TTAACCATTG ACCTTATAAA GGATGGATTC ATTTGGTTGA CATCAGTACA AGTAATTTCT
      541 GCATTTATCC TGTTCTTTCT GTTCTTTTTC AACCAACCTA GTAATAATCC GAATTTAGAA
      601 GTAGTGAGTC CATATCATGA TTTCTGGAAA AGTCTGTGGT TCGCTGTATT CCATTCTACA
      661 AGCGCCGTTA ACAACGCGGG TTTTGATATT ATATCTCCAA ACTCATTGCA ACCCTACAAT
      721 GTTGATAATC ACCGCGTCTA TGCCATACAG GTGATATTTA TGCTGGAGTG GATAATAGGT
      781 GGCCTGGGCT ACCCAACATT CCACGACATA AAAAGAAAGT TGAAAGCTAG AAAAACAAAA
      841 GAGAAGATCA ACTTCTCGCT GTTCACTAAG ATAAACTTTT GGGTATATTT GGTACTGTTC
      901 ATATTCGGGC CCTTAGCGGT GTTTGCCACA GAGTACTCAA ATTATAACAA CAGTTTGATC
      961 TTTCACTATT ACGATGAAAA CTTTACCGTG TTGAATGCCA AGAGTAATAC TGTCGTGTTT
     1021 ATGGATATTC TGTTTAATAC AACGGCAAGC CGCAATGCTG GATTCAGCAC AATTGACATT
     1081 AGCACCTTTA ATTCAGGATC AAAGGCGATA CTGAGCATTT TGATGTTTAT CGGGAGTGCA
     1141 CCAAGTTCTA CGGCAGGCGG TATACGGACA ACCACATTCG GCGTTCTTTT GCTGTCCACA
     1201 TTCACTATTA TTAAGAATCA GAAATTCACT TCAGCGTTCC GGAAAACAAT ACCGAGCGAG
     1261 ACTGTCAATC GCTCGTACGC CGCTTTCTTT ATTTCAACCT TTTTAATTTT TATAGCTTTG
     1321 TTTATTATAT ATGTAGATTC CAATTCCGTG TTCCACACCT TGAAGAACCA TAATTCAGCA
     1381 TCGATCAATA CTATCTTGTT AATCACCTCA GCATTCGGTA CAGTAGGCTT GTCTCCTTTA
     1441 GCTCATTTTC AGATGTATCA GTTAGGCGTG GTAACAAAGA TATCGCTTAT CTTAATCATG
     1501 TTTATCGGGC AGTTAGGTGT CTCCAACACA CTGCTGATAT TTTTAAAGCC AGCACGCGAT
     1561 AAGGCATATA AGTACTTGGA AGAAGATATT ACGATCGGCT GAAGAGCTTA GAGACCGGTA
     1621 CTAGTTCCTG CAGGGCTCGA GGTGTCAATC GTCGGAGCCG CTGAGCAATA ACTAGCATAA
     1681 CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC ATGGTCATAG CTGTTTCCTG
     1741 AGAGCTTGGC AGGTGATGAC ACACATTAAC AAATTTCGTG AGGAGTCTCC AGAAGAATGC
     1801 CATTAATTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA
     1861 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT
     1921 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC
     1981 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT
     2041 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC
     2101 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC
     2161 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG
     2221 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC
     2281 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG
     2341 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA
     2401 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT
     2461 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG
     2521 TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT
     2581 CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC
     2641 CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT
     2701 ACCGCGAGAA CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG
     2761 GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG
     2821 CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC
     2881 TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA
     2941 ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG
     3001 TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC
     3061 ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA
     3121 CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC
     3181 AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG
     3241 TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC
     3301 CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC
     3361 AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT
     3421 ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG
     3481 CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACCTGCAC
     3541 CAGTCAGTAA AACGACGGCC AGTAGTCAAA AGCCTCCGAC CGGAGGCTTT TGACTTGGTC
     3601 AGGTGGAGTG AATTCGCGGC CGCTTCTAGA GGCTCTTCGT AATTACGAAG AGGTCTCTA
//