LOCUS       Exported     3497 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3497
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1510..1557
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3385..3401
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3402..3433
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /label="MMSYN1_0687"
                     /note="GatB: aspartylglutamyl-tRNA(AsnGln) amidotransferase"
                     /translation="MQNFEIIIGVENHVELKTNSKMFSPSKVSYGQTPNTLANEIDLAYPGTLPSVNKKGVELAILACNALNMQIDTLLTFDRKNYFYPDLTKGFQITQQFNPIGTNGSLEITLENGNKKVIEIERLHIEEDTAKQVHKDNLTYLDYNRSGVGLIEIVTKPVLRSAEEACLYVEKLREILLFLNVSDVKMNEGSLRTDLNISLRPYGSDKFSNKVEIKNLNSISNIKKAVEFEINRQKEILLKNQIVEQQTRRFDDQTSSTILMRSKIDSIDYRYFREPNIFPIQLDQKWVDQIISNSPELADQKRIRYVNELGLTSEDANIILTSLEMTNFFEKTIKSTTNYNKVAKMLISEIQAKLNLENKTIDQIKLSPENLASVINLIDKNIISSKQTKVIMPIILDSNTETVEQIVERLNLKLITNKDEISKLLVNIINQNKELLNQYSTRPERVIKTIMGQLMKQTNGNVDPEIANEIVIKEIEKNL"
     primer_bind     complement(1562..1578)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1645..2233)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2404..3264)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
     promoter        complement(3265..3369)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /note="GatB: aspartylglutamyl-tRNA(AsnGln) amidotransferase"
                     /translation="MQNFEIIIGVENHVELKTNSKMFSPSKVSYGQTPNTLANEIDLAYPGTLPSVNKKGVELAILACNALNMQIDTLLTFDRKNYFYPDLTKGFQITQQFNPIGTNGSLEITLENGNKKVIEIERLHIEEDTAKQVHKDNLTYLDYNRSGVGLIEIVTKPVLRSAEEACLYVEKLREILLFLNVSDVKMNEGSLRTDLNISLRPYGSDKFSNKVEIKNLNSISNIKKAVEFEINRQKEILLKNQIVEQQTRRFDDQTSSTILMRSKIDSIDYRYFREPNIFPIQLDQKWVDQIISNSPELADQKRIRYVNELGLTSEDANIILTSLEMTNFFEKTIKSTTNYNKVAKMLISEIQAKLNLENKTIDQIKLSPENLASVINLIDKNIISSKQTKVIMPIILDSNTETVEQIVERLNLKLITNKDEISKLLVNIINQNKELLNQYSTRPERVIKTIMGQLMKQTNGNVDPEIANEIVIKEIEKNL"
                     /label="MMSYN1_0687"
ORIGIN
        1 ATGCAAAACT TCGAAATCAT TATTGGTGTC GAAAACCACG TCGAGCTTAA GACGAACTCA
       61 AAAATGTTCT CCCCTTCCAA GGTAAGCTAC GGACAAACGC CTAACACACT TGCCAACGAA
      121 ATTGACCTTG CATATCCTGG CACGTTACCA AGCGTCAATA AGAAGGGCGT AGAACTGGCG
      181 ATTTTAGCCT GTAACGCATT GAACATGCAA ATCGATACAT TGCTGACGTT CGATCGTAAA
      241 AATTATTTCT ATCCAGACCT TACTAAGGGG TTCCAAATTA CGCAGCAGTT TAACCCTATC
      301 GGAACCAATG GAAGCCTTGA GATTACTCTG GAAAACGGAA ACAAAAAGGT CATCGAAATT
      361 GAGCGTCTGC ACATCGAAGA GGACACGGCT AAACAAGTAC ACAAAGACAA CCTGACGTAC
      421 CTGGACTACA ATCGTAGTGG GGTGGGGTTA ATTGAAATCG TGACCAAGCC CGTTTTGCGC
      481 TCTGCGGAGG AGGCTTGTTT ATACGTCGAG AAGTTGCGTG AGATTCTTCT GTTTCTTAAC
      541 GTGAGCGACG TGAAGATGAA TGAGGGATCT TTGCGTACGG ATCTTAACAT CTCGCTGCGT
      601 CCGTACGGTT CCGACAAGTT CTCGAACAAA GTCGAGATCA AAAATTTGAA CTCCATTTCT
      661 AACATCAAAA AAGCGGTAGA ATTTGAGATC AACCGTCAGA AAGAAATTTT GCTTAAAAAT
      721 CAGATCGTCG AACAACAAAC GCGCCGCTTT GACGACCAGA CGAGTTCGAC TATTCTTATG
      781 CGTTCGAAGA TTGACTCCAT CGATTACCGT TATTTTCGCG AACCTAACAT TTTCCCGATT
      841 CAACTTGACC AGAAATGGGT CGACCAAATT ATCTCAAATT CTCCTGAACT GGCCGATCAG
      901 AAACGTATTC GTTATGTTAA TGAGCTGGGC CTGACTTCGG AGGATGCAAA CATTATTTTG
      961 ACGTCCTTGG AGATGACTAA CTTCTTCGAA AAAACTATCA AGTCTACTAC GAACTATAAT
     1021 AAGGTCGCAA AAATGCTGAT TTCGGAGATC CAAGCTAAAC TTAATTTAGA AAATAAGACT
     1081 ATTGACCAAA TCAAGTTAAG CCCTGAGAAC CTGGCGAGCG TTATCAATCT GATTGACAAG
     1141 AATATTATCT CATCGAAGCA GACTAAAGTT ATTATGCCAA TTATCCTTGA CTCTAATACG
     1201 GAAACAGTTG AACAGATCGT CGAGCGTCTT AACCTGAAGC TTATTACAAA CAAGGACGAA
     1261 ATTTCGAAGT TACTTGTCAA TATCATCAAT CAAAACAAAG AGCTTTTGAA TCAGTACTCA
     1321 ACACGTCCCG AACGCGTTAT CAAGACGATC ATGGGCCAGT TGATGAAGCA AACAAATGGG
     1381 AACGTAGACC CCGAAATCGC AAACGAAATT GTGATCAAGG AAATTGAGAA AAATCTGTGA
     1441 AGAGCTTAGA GACCGGTACT AGTTCCTGCA GGGCTCGAGG TGTCAATCGT CGGAGCCGCT
     1501 GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCAT
     1561 GGTCATAGCT GTTTCCTGAG AGCTTGGCAG GTGATGACAC ACATTAACAA ATTTCGTGAG
     1621 GAGTCTCCAG AAGAATGCCA TTAATTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA
     1681 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT
     1741 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT
     1801 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT
     1861 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC
     1921 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT
     1981 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC
     2041 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT
     2101 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA
     2161 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA
     2221 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA
     2281 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT
     2341 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA
     2401 CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC
     2461 CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG
     2521 CCCCAGTGCT GCAATGATAC CGCGAGAACC ACGCTCACCG GCTCCAGATT TATCAGCAAT
     2581 AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT
     2641 CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG
     2701 CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC
     2761 ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA
     2821 AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC
     2881 ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT
     2941 TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG
     3001 TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT
     3061 GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG
     3121 ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC
     3181 CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC
     3241 GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA
     3301 GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG
     3361 GGTTCCGCGC ACCTGCACCA GTCAGTAAAA CGACGGCCAG TAGTCAAAAG CCTCCGACCG
     3421 GAGGCTTTTG ACTTGGTCAG GTGGAGTGAA TTCGCGGCCG CTTCTAGAGG CTCTTCGTAA
     3481 TTACGAAGAG GTCTCTA
//