LOCUS       Exported     4064 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4064
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      2077..2124
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3952..3968
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 for"
     terminator      3969..4000
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /label="MMSYN1_0690"
                     /note="DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA."
                     /translation="MSKDKALLRINQLKEQLNLWSKQYYVDDNPSVDDIEYDLALKELISLETLYPELITSDSPSQKVGGMVSEKFLKITHKTPMLSLGNVFSFDEFLDFNTQISKVSNTLDNQYVAELKIDGLSISLVYENGSLVSAATRGNGVVGEDVTINVKTIKSIPLKINKKERVEVRGEIYLSKAEFEKINQKRLLNNEDLFINPRNAAAGTLRQLDSKIVASRNLDAFLYYYISDDSNNLTQYQSILKLNELGFKTNKETMLCKNLDEIKAYIDKYTNLKNDLDYQIDGIVFKINDKNLQNSLGFTSKIPKWAIAYKFPAEIKQTKLLDIFATVGRTGKITYNAKLEPVFLMGATISAATLNNAEYIKSKDLRINSIVKIKKAGDVIPEVIEAIKDETFYNLEVFQPILYCPNCHSLLEKNENEVDQFCINSSCSMKILRSLQHFSSREAMNIVSLGDRSLEILFNLKIIQNISDIYRLEEYKDEILAIENFGLKSYLNLIDSINMSKNNSLEKVLFGLGIRHIGSKTAKILARKYQNIDNLMKASYDELIQINSIGESLALSIIDWFKIEDNLKLIDELKSFNINFNYLGAKINSDSIIANKSFVITGTLTRPREEFKTLIENNAGKVIGSISKQTDYLLAGNNVGSKLEKAKKLGVKIIDEQQFFDLLKSEKG"
     primer_bind     complement(2129..2145)
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 rev"
     rep_origin      complement(2212..2800)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2971..3831)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
     promoter        complement(3832..3936)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /label="MMSYN1_0690"
                     /note="DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA."
                     /translation="MSKDKALLRINQLKEQLNLWSKQYYVDDNPSVDDIEYDLALKELISLETLYPELITSDSPSQKVGGMVSEKFLKITHKTPMLSLGNVFSFDEFLDFNTQISKVSNTLDNQYVAELKIDGLSISLVYENGSLVSAATRGNGVVGEDVTINVKTIKSIPLKINKKERVEVRGEIYLSKAEFEKINQKRLLNNEDLFINPRNAAAGTLRQLDSKIVASRNLDAFLYYYISDDSNNLTQYQSILKLNELGFKTNKETMLCKNLDEIKAYIDKYTNLKNDLDYQIDGIVFKINDKNLQNSLGFTSKIPKWAIAYKFPAEIKQTKLLDIFATVGRTGKITYNAKLEPVFLMGATISAATLNNAEYIKSKDLRINSIVKIKKAGDVIPEVIEAIKDETFYNLEVFQPILYCPNCHSLLEKNENEVDQFCINSSCSMKILRSLQHFSSREAMNIVSLGDRSLEILFNLKIIQNISDIYRLEEYKDEILAIENFGLKSYLNLIDSINMSKNNSLEKVLFGLGIRHIGSKTAKILARKYQNIDNLMKASYDELIQINSIGESLALSIIDWFKIEDNLKLIDELKSFNINFNYLGAKINSDSIIANKSFVITGTLTRPREEFKTLIENNAGKVIGSISKQTDYLLAGNNVGSKLEKAKKLGVKIIDEQQFFDLLKSEKG"
ORIGIN
        1 ATGTCAAAAG ATAAGGCCTT ATTGCGCATT AACCAGTTGA AAGAACAGTT AAACCTTTGG
       61 TCGAAGCAGT ATTATGTAGA TGACAACCCA AGCGTCGACG ATATTGAGTA TGACCTTGCA
      121 TTAAAAGAAT TGATTTCCCT GGAGACACTG TACCCTGAAT TAATCACAAG CGACAGCCCC
      181 TCACAAAAAG TCGGCGGTAT GGTTAGCGAG AAGTTCCTGA AAATCACCCA TAAGACGCCA
      241 ATGCTGAGTT TAGGGAATGT GTTTTCATTC GACGAGTTCC TTGATTTTAA TACACAAATC
      301 TCTAAGGTAT CAAACACCTT AGATAATCAA TATGTTGCCG AGCTGAAAAT TGACGGGTTG
      361 TCGATCAGCT TGGTATATGA GAACGGGTCA TTAGTCAGCG CTGCTACCCG CGGCAACGGA
      421 GTTGTAGGAG AGGATGTTAC GATTAATGTG AAAACAATTA AATCGATCCC ATTAAAAATC
      481 AACAAGAAAG AACGCGTAGA AGTCCGTGGG GAGATTTACC TGAGCAAAGC GGAATTTGAG
      541 AAAATCAACC AAAAACGTTT ACTGAACAAC GAGGATTTGT TTATCAACCC ACGCAATGCG
      601 GCTGCGGGTA CCCTGCGCCA ACTGGACTCC AAGATTGTAG CTTCTCGCAA TTTGGATGCA
      661 TTTCTGTATT ACTATATCTC GGACGATTCT AATAATCTTA CCCAATATCA GTCAATCCTT
      721 AAGTTAAACG AGTTGGGTTT TAAGACGAAC AAGGAAACGA TGCTTTGTAA GAATTTGGAT
      781 GAGATTAAGG CGTATATCGA CAAGTATACT AATTTAAAGA ACGACCTTGA CTATCAGATT
      841 GACGGTATTG TGTTCAAGAT TAATGATAAG AATTTGCAAA ACAGCCTGGG GTTTACTTCC
      901 AAGATTCCAA AGTGGGCAAT CGCCTATAAG TTCCCGGCTG AGATTAAGCA GACTAAACTG
      961 TTAGACATTT TTGCTACCGT CGGACGCACT GGCAAGATCA CCTATAATGC AAAATTAGAA
     1021 CCCGTGTTCT TGATGGGTGC TACAATCAGT GCTGCGACAT TAAATAACGC TGAATATATT
     1081 AAGTCGAAAG ATTTGCGTAT TAATTCAATC GTAAAAATTA AGAAAGCGGG GGACGTTATC
     1141 CCTGAGGTAA TTGAGGCGAT TAAGGACGAA ACATTTTATA ACTTAGAGGT ATTTCAACCG
     1201 ATTCTGTACT GTCCAAATTG CCACAGCTTA CTGGAGAAAA ATGAAAACGA AGTAGACCAA
     1261 TTCTGTATTA ACAGCTCTTG CTCCATGAAG ATTCTTCGCA GCTTGCAGCA TTTTTCCAGC
     1321 CGCGAAGCCA TGAACATTGT AAGTCTGGGC GATCGCTCAT TAGAGATTCT GTTTAATCTT
     1381 AAGATTATTC AGAACATTTC TGATATTTAC CGCTTAGAGG AGTATAAAGA TGAGATCCTT
     1441 GCAATCGAGA ACTTTGGCTT AAAGAGTTAT CTTAATCTGA TCGACTCCAT CAACATGTCC
     1501 AAAAATAACT CGCTGGAAAA AGTCCTTTTT GGGTTAGGTA TCCGCCACAT TGGTTCCAAG
     1561 ACAGCGAAGA TCCTTGCACG CAAGTATCAA AACATTGACA ACTTGATGAA AGCAAGCTAT
     1621 GATGAGTTGA TTCAGATCAA CTCGATCGGA GAGAGTCTTG CGCTTTCGAT TATCGATTGG
     1681 TTCAAAATTG AGGACAATTT GAAATTAATT GACGAGCTGA AATCCTTTAA CATTAACTTT
     1741 AACTACCTGG GAGCAAAGAT CAATTCGGAT AGTATCATTG CAAACAAGAG CTTTGTTATC
     1801 ACCGGGACAT TGACCCGCCC TCGTGAAGAG TTCAAGACGC TGATTGAAAA CAATGCAGGG
     1861 AAAGTGATTG GGAGTATTTC GAAGCAAACC GATTATCTTT TGGCGGGAAA CAACGTTGGT
     1921 AGTAAGTTAG AGAAGGCGAA AAAATTAGGC GTCAAAATCA TCGACGAACA ACAGTTCTTC
     1981 GACTTGCTTA AATCAGAGAA AGGCTGAAGA GCTTAGAGAC CGGTACTAGT TCCTGCAGGG
     2041 CTCGAGGTGT CAATCGTCGG AGCCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT
     2101 AAACGGGTCT TGAGGGGTTT TTTGCATGGT CATAGCTGTT TCCTGAGAGC TTGGCAGGTG
     2161 ATGACACACA TTAACAAATT TCGTGAGGAG TCTCCAGAAG AATGCCATTA ATTTCCATAG
     2221 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC
     2281 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT
     2341 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT
     2401 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG
     2461 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT
     2521 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT
     2581 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG
     2641 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA
     2701 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT
     2761 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC
     2821 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT
     2881 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA
     2941 AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT
     3001 CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC
     3061 TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGAACCACG
     3121 CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG
     3181 TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT
     3241 AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT
     3301 GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT
     3361 TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT
     3421 CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT
     3481 TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT
     3541 CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC
     3601 CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA
     3661 ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA
     3721 CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA
     3781 AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT
     3841 TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA
     3901 ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACC TGCACCAGTC AGTAAAACGA
     3961 CGGCCAGTAG TCAAAAGCCT CCGACCGGAG GCTTTTGACT TGGTCAGGTG GAGTGAATTC
     4021 GCGGCCGCTT CTAGAGGCTC TTCGTAATTA CGAAGAGGTC TCTA
//