LOCUS       Exported     4079 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4079
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      2092..2139
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
                     /label="T7 terminator"
     primer_bind     3967..3983
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3984..4015
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /label="MMSYN1_0691"
                     /note="membrane protein, putative"
                     /translation="MQVNVESTTANMPINDSKKTTSAKSGVFSALLGVVSSITNMIIQFLLIYWVLQSFGTEISGFIRISMSLSIIGGTAEGALALSTVLMLTEPLSKKDWITVNEIFSTAKRNYNNKIVSGFILVFLLSILYPLQIAISPLITSGESIKWGIDFTTPLSKTTSTLKFWELSAVFLILGTKQTLLAGLFGVHENIMQADQKNASKKLVVLFCDVLFYGIFFVLLNSYIYWNDKHTPVLLFLPFLFYPVIRGLLITSYVKKKYPAIKFYNDFNNLNLIRRSTKIYWSSIGQSILVNSDLIIIFLALGSIGLKVSSLISLYMVVAINLRIIMTSLVTSFKEYFSSVIIKKGRLDWETYSNYEFYSYIVGVFSFLITSIMTPYIVTGLFSKIILNDVDTTGLTKKTIEFIIFSPFFSGIFGATTGLIVLLESKITLIHAKGMHRTIAKPLNLIAFSFFISSFIITLLLNRFIGNVESKISWVIIVFYSSKILFLIIAYIYLWIFSWDKLVYNARFNRIIPNILFVTLSACLVIAFSLSADDIYILLKFDTNKKVPVDILHIILGLIIIFIASFFIGILTFVYNKIVKNTSVTRLIFYSLPFIKRLNKEKQEKAKRDLFEKENINIDKFLLKQEDLLKAMYGFKEKKVIDQDEFEKYSKYKPKPKVYILKASDMNKDESEY"
     primer_bind     complement(2144..2160)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(2227..2815)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2986..3846)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
     promoter        complement(3847..3951)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /label="MMSYN1_0691"
                     /note="membrane protein, putative"
                     /translation="MQVNVESTTANMPINDSKKTTSAKSGVFSALLGVVSSITNMIIQFLLIYWVLQSFGTEISGFIRISMSLSIIGGTAEGALALSTVLMLTEPLSKKDWITVNEIFSTAKRNYNNKIVSGFILVFLLSILYPLQIAISPLITSGESIKWGIDFTTPLSKTTSTLKFWELSAVFLILGTKQTLLAGLFGVHENIMQADQKNASKKLVVLFCDVLFYGIFFVLLNSYIYWNDKHTPVLLFLPFLFYPVIRGLLITSYVKKKYPAIKFYNDFNNLNLIRRSTKIYWSSIGQSILVNSDLIIIFLALGSIGLKVSSLISLYMVVAINLRIIMTSLVTSFKEYFSSVIIKKGRLDWETYSNYEFYSYIVGVFSFLITSIMTPYIVTGLFSKIILNDVDTTGLTKKTIEFIIFSPFFSGIFGATTGLIVLLESKITLIHAKGMHRTIAKPLNLIAFSFFISSFIITLLLNRFIGNVESKISWVIIVFYSSKILFLIIAYIYLWIFSWDKLVYNARFNRIIPNILFVTLSACLVIAFSLSADDIYILLKFDTNKKVPVDILHIILGLIIIFIASFFIGILTFVYNKIVKNTSVTRLIFYSLPFIKRLNKEKQEKAKRDLFEKENINIDKFLLKQEDLLKAMYGFKEKKVIDQDEFEKYSKYKPKPKVYILKASDMNKDESEY"
ORIGIN
        1 ATGCAAGTGA ACGTAGAAAG CACGACAGCG AACATGCCAA TCAATGACAG CAAGAAAACA
       61 ACATCAGCAA AGAGCGGAGT GTTCTCAGCA TTACTGGGCG TTGTCTCATC CATCACTAAT
      121 ATGATTATTC AATTCTTGTT AATCTATTGG GTGTTACAAT CATTTGGCAC TGAAATCTCA
      181 GGATTTATCC GCATCTCGAT GAGCTTATCG ATCATTGGCG GTACAGCCGA AGGGGCGTTA
      241 GCCCTTTCAA CGGTCCTGAT GCTTACTGAA CCCTTATCTA AAAAGGACTG GATTACCGTT
      301 AATGAAATCT TTTCGACTGC GAAACGTAAT TATAATAACA AAATTGTAAG CGGTTTCATC
      361 CTGGTTTTTC TTTTATCAAT CCTTTATCCT CTTCAGATCG CAATCAGCCC TTTAATTACA
      421 TCCGGGGAAT CCATTAAATG GGGCATTGAT TTTACCACGC CATTATCAAA GACTACTTCT
      481 ACACTGAAGT TTTGGGAACT TTCCGCAGTA TTTTTGATTC TTGGAACCAA ACAGACTCTG
      541 TTAGCAGGAC TGTTCGGAGT CCATGAAAAC ATTATGCAAG CCGATCAAAA GAACGCGTCG
      601 AAAAAATTGG TTGTACTTTT CTGCGACGTG TTATTTTATG GAATCTTTTT CGTCTTACTG
      661 AACTCCTACA TTTACTGGAA TGACAAGCAT ACGCCCGTTC TGCTTTTTCT GCCTTTTCTG
      721 TTCTATCCGG TAATTCGCGG GCTGCTGATC ACCAGCTACG TGAAGAAAAA ATATCCCGCC
      781 ATCAAATTCT ACAATGATTT TAATAATCTT AACTTAATTC GCCGCTCCAC AAAGATTTAC
      841 TGGTCTAGTA TTGGCCAGTC GATTCTGGTC AACTCTGACC TGATTATCAT CTTTTTAGCA
      901 CTTGGGAGTA TTGGTTTGAA GGTTTCTAGC TTAATCTCCC TGTACATGGT TGTTGCTATT
      961 AACCTTCGTA TTATCATGAC CTCGCTGGTC ACCTCTTTTA AGGAGTACTT TTCATCCGTT
     1021 ATCATCAAGA AAGGCCGCCT TGATTGGGAA ACCTATTCGA ATTACGAATT TTATTCATAC
     1081 ATCGTCGGTG TATTCTCTTT TTTAATCACT TCTATTATGA CTCCATATAT TGTCACAGGA
     1141 TTGTTTAGTA AGATCATCTT GAACGACGTT GACACAACCG GCTTAACCAA AAAGACCATT
     1201 GAATTCATCA TCTTTAGCCC CTTCTTTTCA GGGATCTTTG GGGCGACGAC AGGTTTAATT
     1261 GTGCTTTTAG AAAGCAAGAT CACGCTGATC CACGCTAAAG GAATGCATCG TACAATCGCC
     1321 AAACCCTTAA ATTTGATCGC CTTTTCCTTT TTTATTAGCT CGTTTATTAT TACGTTGTTA
     1381 CTGAACCGTT TCATCGGCAA CGTAGAAAGC AAGATTAGTT GGGTGATTAT TGTGTTCTAT
     1441 AGTTCTAAGA TTTTATTTTT GATTATTGCC TACATTTATT TATGGATCTT TTCTTGGGAT
     1501 AAGTTGGTAT ACAACGCGCG TTTTAACCGT ATCATCCCCA ACATCTTATT CGTTACCTTG
     1561 AGTGCGTGCC TGGTTATTGC GTTTAGTTTA TCCGCAGATG ATATCTATAT CTTGTTGAAA
     1621 TTTGATACAA ATAAGAAAGT CCCAGTCGAC ATCCTTCACA TCATCTTAGG CCTTATTATT
     1681 ATCTTTATCG CTTCCTTTTT TATTGGAATC TTGACCTTCG TCTATAACAA AATCGTTAAA
     1741 AATACTTCTG TCACTCGCCT TATTTTTTAT TCCCTTCCTT TTATCAAGCG TCTGAATAAG
     1801 GAGAAACAAG AGAAAGCAAA GCGCGACCTT TTCGAAAAGG AAAATATTAA TATTGATAAG
     1861 TTTCTGCTGA AACAAGAGGA CTTGCTGAAA GCCATGTATG GGTTCAAGGA GAAAAAAGTA
     1921 ATTGACCAAG ACGAGTTCGA AAAATACTCG AAATACAAAC CCAAACCTAA GGTCTACATT
     1981 CTTAAAGCGT CAGATATGAA CAAGGATGAG AGTGAGTACT GAAGAGCTTA GAGACCGGTA
     2041 CTAGTTCCTG CAGGGCTCGA GGTGTCAATC GTCGGAGCCG CTGAGCAATA ACTAGCATAA
     2101 CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC ATGGTCATAG CTGTTTCCTG
     2161 AGAGCTTGGC AGGTGATGAC ACACATTAAC AAATTTCGTG AGGAGTCTCC AGAAGAATGC
     2221 CATTAATTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA
     2281 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT
     2341 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC
     2401 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT
     2461 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC
     2521 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC
     2581 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG
     2641 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC
     2701 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG
     2761 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA
     2821 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT
     2881 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG
     2941 TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT
     3001 CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC
     3061 CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT
     3121 ACCGCGAGAA CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG
     3181 GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG
     3241 CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC
     3301 TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA
     3361 ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG
     3421 TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC
     3481 ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA
     3541 CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC
     3601 AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG
     3661 TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC
     3721 CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC
     3781 AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT
     3841 ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG
     3901 CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACCTGCAC
     3961 CAGTCAGTAA AACGACGGCC AGTAGTCAAA AGCCTCCGAC CGGAGGCTTT TGACTTGGTC
     4021 AGGTGGAGTG AATTCGCGGC CGCTTCTAGA GGCTCTTCGT AATTACGAAG AGGTCTCTA
//