LOCUS       Exported     2966 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2966
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      979..1026
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2854..2870
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2871..2902
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /label="MMSYN1_0692"
                     /note=""
                     /translation="MTKFVVNKNDQNQTLFKFLKKTFKTTPISVIYKWIRNKSIKINSKRISDKNYLLKINDVIEVYDSNKPIIRDQFNYISNVNLDIVYEDNNILIVNKPNNLEMHSTYNLCLDDMVKSYLVDKKEYDIYLENSFVISHVHRLDKLTSGLVIYAKNKISSTILTNAFKSKDQINKYYYALTSSDWSLDEFLQVNGYINYDSNIKKADFSLDKKNNYKYCQTEFKLINKNLILVKLITGKKHQIRSVLSFYNHPILNDFRYNGKKINDLKMIYLSAFKIEFKNLEKPLDYLNNKVFIKNPEWISKE"
     primer_bind     complement(1031..1047)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1114..1702)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1873..2733)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2734..2838)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /label="MMSYN1_0692"
                     /note=""
                     /translation="MTKFVVNKNDQNQTLFKFLKKTFKTTPISVIYKWIRNKSIKINSKRISDKNYLLKINDVIEVYDSNKPIIRDQFNYISNVNLDIVYEDNNILIVNKPNNLEMHSTYNLCLDDMVKSYLVDKKEYDIYLENSFVISHVHRLDKLTSGLVIYAKNKISSTILTNAFKSKDQINKYYYALTSSDWSLDEFLQVNGYINYDSNIKKADFSLDKKNNYKYCQTEFKLINKNLILVKLITGKKHQIRSVLSFYNHPILNDFRYNGKKINDLKMIYLSAFKIEFKNLEKPLDYLNNKVFIKNPEWISKE"
ORIGIN
        1 ATGACCAAAT TCGTCGTGAA TAAGAATGAC CAGAATCAAA CGTTGTTCAA ATTCCTGAAA
       61 AAGACTTTCA AAACGACCCC TATTTCAGTT ATTTACAAGT GGATTCGCAA CAAGTCGATC
      121 AAGATTAACT CGAAACGCAT CAGCGACAAA AACTATTTAC TGAAGATTAA CGATGTGATC
      181 GAGGTCTATG ATTCAAACAA ACCAATTATT CGCGATCAAT TCAATTATAT TAGTAACGTG
      241 AACTTAGATA TTGTGTACGA GGACAACAAC ATTTTAATTG TCAACAAACC TAATAATTTG
      301 GAAATGCACT CGACTTACAA TCTTTGCTTG GACGACATGG TGAAATCGTA TCTTGTGGAC
      361 AAAAAGGAAT ATGATATTTA CCTTGAGAAT AGCTTTGTCA TCTCTCATGT TCATCGTCTG
      421 GACAAACTGA CAAGTGGCTT GGTTATTTAC GCTAAAAATA AAATTTCTTC TACAATCTTG
      481 ACCAACGCGT TCAAGTCGAA AGATCAGATC AATAAATATT ACTATGCTTT AACGAGTAGC
      541 GATTGGAGTC TTGACGAGTT TCTTCAGGTG AATGGATATA TCAACTACGA TTCAAACATT
      601 AAAAAGGCTG ACTTTTCCCT GGACAAGAAA AACAACTATA AGTATTGCCA AACGGAATTC
      661 AAGTTAATCA ACAAAAACCT TATTCTTGTT AAACTTATTA CCGGCAAGAA GCACCAAATC
      721 CGTTCAGTCC TTTCTTTTTA TAACCACCCA ATTCTGAACG ATTTCCGTTA CAATGGTAAG
      781 AAAATCAACG ACCTGAAAAT GATTTACCTG AGTGCATTCA AAATTGAATT CAAGAATTTG
      841 GAGAAACCAC TGGATTATTT GAATAATAAA GTGTTTATTA AGAATCCCGA ATGGATCTCA
      901 AAAGAGTGAA GAGCTTAGAG ACCGGTACTA GTTCCTGCAG GGCTCGAGGT GTCAATCGTC
      961 GGAGCCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT
     1021 TTTTTGCATG GTCATAGCTG TTTCCTGAGA GCTTGGCAGG TGATGACACA CATTAACAAA
     1081 TTTCGTGAGG AGTCTCCAGA AGAATGCCAT TAATTTCCAT AGGCTCCGCC CCCCTGACGA
     1141 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA
     1201 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC
     1261 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG
     1321 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC
     1381 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG
     1441 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT
     1501 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT
     1561 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG
     1621 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC
     1681 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA
     1741 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC
     1801 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC
     1861 TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT
     1921 TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT
     1981 ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGAACCA CGCTCACCGG CTCCAGATTT
     2041 ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC
     2101 CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA
     2161 TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG
     2221 TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT
     2281 GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC
     2341 AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT
     2401 AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG
     2461 GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC
     2521 TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC
     2581 GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT
     2641 TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG
     2701 AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG
     2761 CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA
     2821 ACAAATAGGG GTTCCGCGCA CCTGCACCAG TCAGTAAAAC GACGGCCAGT AGTCAAAAGC
     2881 CTCCGACCGG AGGCTTTTGA CTTGGTCAGG TGGAGTGAAT TCGCGGCCGC TTCTAGAGGC
     2941 TCTTCGTAAT TACGAAGAGG TCTCTA
//