LOCUS       Exported     4192 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4192
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2010..4178
                     /label="MMSYN1_0695"
                     /note="PcrA: ATP-dependent DNA helicase"
                     /translation="MSVDHLLDLLNPQQLAAVINTDKPVRIIAGAGSGKTRVITTKIAYLIEKKHIDPTRILAVTFTNKAAKEMKERVLQITKNQKKSPFISTFHAWCSKVLRIDGKHVGLKDKFLIIDSDDQKRIIKNALKESNIELSENDKKTFDKKILYKIKEWKEELVDPDEAILNANSTYDRNSAIIYKLYQETLLKNNSIDFDDLQIYVYLLFKNHQEILNKWRNAYDYVLVDEFQDTNDIQFSLIKFLTINTNHLTVVGDPDQTIYSWRGAKLDIILNFNKTYSNAISIVLNQNYRSTKQILDISNSFIKNNKFREHKEIFTNNKSGKKVVLKECNSKTSEASYVSFKIKELIKQGYHYKDIFILYRMNAWSQEFEKELINKKIPFQLIGGIKFRERKVIKDAMAFLKMISIKDDLSSQRVLSLIPKIGNITIEKIINIANLNHISIFDLITNEDKTLLQSITKNLDELIEVFKTAHQLYLDNTNIEEILKYLLIQSGYENKLKVKNEHDDLDNINALYDQLKRFDENFDANYYSEDNKLIAFLQEEALTSDIDEAQQIDKVSLLTVHAAKGLENKIVFITGLNQGIFPTRLSENNQKELEEERRALYVALTRAKEELFLTYVKGDYSHIMQSELKPSKFIHELDKDLYEFESQFLNSQIYDKNQHKTPSFYVSPKQHNLYNVGDYVEHKLFGKGIITKVINDQLQISFTNSSYGIMIIAANNSALTKL"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GGGTCTCAAA TGTCTGTCGA CCACCTGTTG GACTTATTGA
     2041 ATCCGCAACA ACTGGCAGCA GTCATTAACA CTGACAAACC AGTGCGCATT ATCGCCGGCG
     2101 CAGGCTCCGG CAAAACACGC GTTATTACTA CGAAGATTGC CTATTTGATT GAGAAAAAGC
     2161 ACATCGATCC GACTCGCATC CTGGCAGTAA CCTTCACGAA CAAGGCCGCG AAGGAGATGA
     2221 AAGAACGCGT ACTTCAGATT ACTAAAAACC AGAAGAAATC ACCGTTCATT TCCACTTTTC
     2281 ATGCCTGGTG TTCAAAAGTG CTGCGCATCG ACGGAAAGCA TGTCGGGTTG AAAGATAAAT
     2341 TCCTTATCAT TGACAGTGAT GACCAAAAAC GCATTATTAA GAACGCTCTG AAGGAAAGTA
     2401 ATATTGAATT ATCCGAAAAT GATAAGAAAA CATTCGACAA GAAAATTTTG TACAAGATTA
     2461 AGGAATGGAA GGAAGAATTG GTAGACCCCG ACGAAGCTAT CTTGAACGCA AACTCGACTT
     2521 ACGATCGCAA CTCAGCAATC ATCTATAAAT TATATCAAGA GACTTTACTG AAAAATAATT
     2581 CCATCGACTT CGACGACCTT CAGATTTATG TCTATTTGCT GTTCAAGAAC CACCAAGAGA
     2641 TCTTGAATAA GTGGCGCAAC GCGTATGATT ATGTGTTGGT CGACGAGTTT CAGGACACTA
     2701 ATGATATCCA GTTTTCGTTG ATCAAGTTCC TGACGATCAA CACAAACCAC CTGACGGTCG
     2761 TCGGTGACCC GGACCAAACT ATTTATAGCT GGCGCGGTGC TAAGCTGGAC ATCATTCTGA
     2821 ACTTTAACAA GACCTATAGC AACGCAATTA GCATTGTACT TAATCAGAAT TACCGCTCCA
     2881 CTAAGCAAAT CCTTGATATC AGCAACTCTT TTATTAAGAA CAACAAATTT CGCGAGCACA
     2941 AAGAAATTTT CACAAACAAC AAGTCCGGAA AGAAAGTGGT TTTAAAGGAG TGCAATAGCA
     3001 AAACCAGCGA GGCGTCCTAT GTCAGTTTCA AGATTAAGGA GTTAATCAAA CAAGGGTACC
     3061 ACTATAAAGA CATTTTCATT TTATACCGCA TGAACGCTTG GTCCCAGGAA TTTGAGAAAG
     3121 AGCTTATTAA CAAAAAGATC CCATTCCAAT TGATTGGTGG GATTAAGTTT CGCGAGCGCA
     3181 AGGTGATCAA AGACGCAATG GCGTTTCTGA AAATGATCTC CATCAAAGAT GACTTGAGTT
     3241 CGCAGCGTGT ACTTTCTTTG ATTCCTAAAA TTGGGAATAT CACCATCGAA AAGATCATCA
     3301 ATATCGCAAA TTTGAATCAT ATCTCCATTT TTGATCTTAT CACAAACGAG GACAAAACAC
     3361 TTCTGCAATC CATCACTAAA AACTTGGATG AGTTAATTGA AGTATTTAAG ACAGCGCACC
     3421 AACTGTATCT TGACAATACA AATATCGAGG AAATTTTGAA GTACTTACTT ATCCAAAGCG
     3481 GATATGAGAA TAAACTTAAA GTAAAGAACG AACACGACGA TTTAGACAAT ATCAACGCGT
     3541 TGTATGATCA ATTGAAGCGT TTTGATGAAA ATTTTGACGC GAACTATTAT AGCGAGGACA
     3601 ATAAACTGAT TGCCTTCTTA CAGGAAGAAG CCCTGACGTC CGACATTGAC GAGGCGCAGC
     3661 AGATCGACAA AGTATCCTTG TTGACCGTGC ATGCTGCCAA AGGTTTAGAG AACAAGATCG
     3721 TCTTTATTAC CGGGTTGAAC CAGGGTATCT TTCCTACACG TCTTAGTGAG AATAACCAGA
     3781 AAGAGTTAGA AGAGGAGCGT CGTGCCCTGT ATGTAGCCTT AACACGTGCT AAAGAGGAAC
     3841 TTTTTCTGAC CTACGTAAAG GGTGATTACT CCCATATCAT GCAATCTGAG CTTAAACCGT
     3901 CCAAATTCAT TCATGAGCTG GACAAGGATT TGTATGAATT CGAGTCTCAA TTTTTGAACT
     3961 CTCAGATCTA CGATAAAAAT CAGCACAAAA CTCCGTCGTT TTATGTCTCT CCCAAGCAAC
     4021 ACAACTTATA TAACGTAGGG GACTATGTAG AACACAAACT GTTTGGAAAG GGCATTATTA
     4081 CTAAGGTGAT CAACGACCAA CTTCAGATTA GCTTCACGAA TTCGAGTTAC GGCATCATGA
     4141 TTATTGCTGC AAACAATTCT GCGCTTACTA AGCTGTGAAG AGCTTAGAGA CC
//