LOCUS       Exported     3772 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3772
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2010..3758
                     /note="ABC transporter, permease protein"
                     /translation="MATRQNYKKPFLVKESNFFKYRYINRTTNTKTSWKFHPIYFHLLAFLVLVLVGYCFYSQASLIKIDNFYQVAKKLVLLFSFENKNFLDTSYISNEYTNLFLDTLSLLWVTIKLALTGTFIGFILAVITSFLSFSKVNNKFLSYLLSAVILILRSTPELIFITLITSTFRNDLSLLLVYIWFTWLWLHKYYIDMLNSFDLQAYYVSISQGNSKFKAFFKEIYPRIKNRVIALFIFSFESNIRWASILAALSLPGIGRLIVYGSENTAHFNQLGIPLLVLMSFILVLELLNYLFKKYLVEARSKVYKQKNETKFEYYTRLSKKLNVNKIIISLIFISLTIISIITFINIPIYIFNLDYVKSFFNNLLNPNFVSFSIFNKHIENNPILLIWNSLQFTIVAMFICIVITIIGIRLQSIRLNNLFVVIICRSLNVLIRLIPTIVYFYVFHPIFSNVLTLVIIVVSLHQASSKAKQLVEVVDNLNIQIINNLKIQGYSNNQIFLKYVLPAIKIEFISLSIFYFELIFRTSITYYILASDKLYIGHLITKYLDTRAFYPRLAMSYVWIGTFAILVINLIARYINKKIRK"
                     /label="MMSYN1_0706"
     primer_bind     complement(90..106)
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 rev"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GGGTCTCAAA TGGCGACGCG CCAGAACTAT AAGAAGCCTT
     2041 TCTTGGTGAA GGAGTCGAAT TTCTTTAAGT ATCGCTATAT TAATCGCACT ACCAATACCA
     2101 AGACAAGTTG GAAATTCCAC CCAATTTACT TTCATTTGTT AGCATTCTTG GTGTTGGTTT
     2161 TAGTGGGCTA CTGTTTTTAT TCGCAAGCCA GTTTAATTAA GATCGACAAT TTTTACCAAG
     2221 TTGCGAAGAA GTTGGTGTTG TTGTTCAGCT TTGAGAATAA AAATTTCCTT GATACCTCCT
     2281 ACATCTCCAA TGAATACACC AACCTTTTCT TGGACACGTT GAGCTTATTA TGGGTAACTA
     2341 TCAAATTGGC TCTGACCGGG ACATTCATCG GCTTCATCCT TGCGGTAATT ACCTCGTTCC
     2401 TGTCATTCTC TAAAGTCAAC AATAAATTTT TATCGTATTT GCTGTCGGCT GTGATCCTGA
     2461 TTCTGCGCTC TACACCAGAG TTGATTTTTA TTACCTTAAT TACGTCAACT TTCCGCAACG
     2521 ACCTTTCTTT ATTATTGGTA TATATCTGGT TCACATGGCT GTGGCTGCAC AAATACTACA
     2581 TTGACATGCT TAACTCGTTT GATCTGCAAG CATATTACGT GTCAATTTCG CAGGGTAACT
     2641 CCAAATTCAA AGCCTTTTTC AAAGAGATTT ATCCACGTAT CAAAAACCGC GTAATTGCGT
     2701 TGTTCATTTT CTCATTCGAA TCAAATATTC GTTGGGCATC CATCTTGGCA GCCTTGTCTC
     2761 TTCCCGGTAT CGGTCGCTTA ATTGTATATG GCTCAGAGAA CACGGCGCAT TTTAATCAAC
     2821 TGGGCATTCC TTTGTTGGTA CTTATGAGTT TTATCCTGGT CTTAGAGTTG TTAAACTATT
     2881 TATTTAAGAA ATATTTAGTG GAAGCACGTT CAAAAGTTTA TAAGCAGAAA AATGAAACTA
     2941 AATTTGAATA TTACACCCGC CTGTCCAAGA AACTTAACGT TAACAAGATC ATTATTTCAT
     3001 TGATTTTTAT TAGCCTGACT ATTATCTCTA TTATCACGTT CATTAACATC CCTATCTATA
     3061 TTTTCAATCT TGATTACGTT AAGAGCTTCT TTAACAATTT GCTGAATCCG AACTTTGTAA
     3121 GTTTTAGCAT TTTTAATAAG CATATTGAAA ACAACCCTAT TTTACTTATC TGGAATAGTT
     3181 TGCAATTCAC GATCGTGGCC ATGTTCATCT GTATCGTGAT CACTATCATC GGTATCCGCC
     3241 TTCAATCCAT TCGTCTTAAT AACCTGTTTG TCGTCATTAT CTGCCGTTCG CTTAATGTGT
     3301 TGATCCGTTT AATTCCGACC ATCGTCTACT TTTACGTCTT TCACCCAATC TTCTCTAATG
     3361 TGTTGACTTT AGTGATTATC GTTGTATCTT TGCACCAAGC TAGCTCCAAA GCAAAGCAAC
     3421 TTGTTGAGGT TGTGGATAAT CTGAACATTC AGATTATTAA TAATCTTAAA ATTCAGGGAT
     3481 ATTCCAATAA TCAGATTTTC CTTAAATACG TCCTGCCCGC GATCAAGATC GAATTTATCA
     3541 GTTTGTCGAT TTTTTATTTC GAGCTTATTT TCCGTACCTC GATTACATAC TATATTCTTG
     3601 CCTCCGACAA ATTGTACATT GGCCATCTGA TTACCAAATA CCTGGACACT CGCGCCTTCT
     3661 ATCCCCGTCT TGCAATGAGC TACGTGTGGA TTGGAACATT TGCCATCTTG GTAATCAATC
     3721 TTATCGCACG TTACATCAAT AAGAAAATTC GCAAGTGAAG AGCTTAGAGA CC
//