LOCUS       Exported     2807 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2807
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
                     /label="T7 terminator"
     terminator      820..867
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2695..2711
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2712..2743
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /translation="MNKVIELKEISVQYNNRSDLVLKDINLDIFQGELVAIIGPSGVGKSTLFKIIINSLRPVKGQVKVFDKDILKFNKKQKRLFISKIGFLTQTPNLIYTDNVYNNIIRSTSKYKNNFYKFFSILTRKQKITIFEKLDELNILDKAFFKVSELSGGQQQRVEIAKLLIKDVELILADEPTSNLDKKTSIEVLKVLKNISKQNKTILVNIHDLSLVKRYFDRVIAINNKQIVFDKKTKDIKQWQLDRIIKSRS"
                     /label="MMSYN1_0707"
                     /note=""
     primer_bind     complement(872..888)
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 rev"
     rep_origin      complement(955..1543)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1714..2574)
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
     promoter        complement(2575..2679)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /note=""
                     /translation="MNKVIELKEISVQYNNRSDLVLKDINLDIFQGELVAIIGPSGVGKSTLFKIIINSLRPVKGQVKVFDKDILKFNKKQKRLFISKIGFLTQTPNLIYTDNVYNNIIRSTSKYKNNFYKFFSILTRKQKITIFEKLDELNILDKAFFKVSELSGGQQQRVEIAKLLIKDVELILADEPTSNLDKKTSIEVLKVLKNISKQNKTILVNIHDLSLVKRYFDRVIAINNKQIVFDKKTKDIKQWQLDRIIKSRS"
                     /label="MMSYN1_0707"
ORIGIN
        1 ATGAACAAGG TAATTGAGCT GAAAGAAATC AGTGTGCAGT ATAACAACCG TTCGGACTTA
       61 GTTCTGAAAG ATATCAACTT GGACATCTTT CAGGGCGAGT TGGTAGCGAT TATCGGACCC
      121 TCGGGAGTAG GTAAGTCTAC TTTATTTAAG ATCATTATCA ATTCACTTCG CCCAGTGAAA
      181 GGTCAGGTAA AAGTCTTTGA TAAGGACATT CTGAAGTTCA ATAAAAAGCA GAAGCGCCTT
      241 TTTATTAGTA AGATTGGCTT TCTTACCCAG ACACCTAATT TAATCTACAC AGATAATGTT
      301 TATAACAATA TCATCCGTTC AACCTCAAAG TATAAAAACA ACTTCTATAA GTTCTTTAGT
      361 ATCTTAACGC GTAAACAAAA AATTACCATT TTCGAGAAAC TTGACGAATT GAATATTTTG
      421 GACAAAGCCT TCTTCAAGGT GTCGGAATTA TCGGGCGGTC AGCAACAACG TGTGGAAATT
      481 GCCAAACTGC TGATCAAGGA TGTTGAGTTG ATTCTTGCAG ATGAGCCAAC ATCCAACCTT
      541 GATAAAAAGA CTTCCATCGA AGTGTTAAAG GTTTTGAAAA ACATTAGTAA ACAGAATAAG
      601 ACTATCTTGG TAAATATTCA TGATTTATCC CTTGTAAAGC GTTACTTTGA TCGCGTGATT
      661 GCGATTAACA ATAAACAAAT TGTCTTTGAT AAAAAGACGA AAGATATTAA ACAGTGGCAA
      721 TTAGATCGTA TTATCAAGAG TCGTTCCTGA AGAGCTTAGA GACCGGTACT AGTTCCTGCA
      781 GGGCTCGAGG TGTCAATCGT CGGAGCCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC
      841 TCTAAACGGG TCTTGAGGGG TTTTTTGCAT GGTCATAGCT GTTTCCTGAG AGCTTGGCAG
      901 GTGATGACAC ACATTAACAA ATTTCGTGAG GAGTCTCCAG AAGAATGCCA TTAATTTCCA
      961 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA
     1021 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC
     1081 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC
     1141 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT
     1201 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG
     1261 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG
     1321 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA
     1381 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG
     1441 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT
     1501 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT
     1561 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG
     1621 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT
     1681 CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC
     1741 TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT
     1801 AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGAACC
     1861 ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG
     1921 AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG
     1981 AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT
     2041 GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG
     2101 AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT
     2161 TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC
     2221 TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC
     2281 ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA
     2341 TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG
     2401 AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC
     2461 CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG
     2521 GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT
     2581 CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT
     2641 TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACCTGCACCA GTCAGTAAAA
     2701 CGACGGCCAG TAGTCAAAAG CCTCCGACCG GAGGCTTTTG ACTTGGTCAG GTGGAGTGAA
     2761 TTCGCGGCCG CTTCTAGAGG CTCTTCGTAA TTACGAAGAG GTCTCTA
//