LOCUS       Exported     3521 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3521
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1534..1581
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3409..3425
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3426..3457
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /label="MMSYN1_0708"
                     /note="high affinity transport system protein p37"
                     /translation="MYTNKLKVALGTMLSAVSIASVGSFVVACQTKEDWDTTITINNSWVNDGFFSKLDYDTGAVTPGDKSKAFIELLTKKFNELKNKDEATKKFKDVKFDIKVDFDKKTYFSKLEKNDSENDVYIANYSYYLSNVWNNKTKSLNKDLPFKLVSQAATLQFNWQSGDNTFYKDGKSTDDLRKLAEENNKKWLEFGEYPDWHKSDKAVNGKKLDFDGSKYTNFYKDVDLTYVYRGAVLIAGNEADREKIVKAWDDKNWDSFVKNGIVYEKTSSAGGYKYQVALFARHFGKTISEIKEDLEGKKYEQYIVKGQKVSAQLGKKQANSQLVPRIGFDDEGSYNWTKSEEGSEKYKPTDFKSTEKAMNGGKAMMTAAKPAAAKPAAAPAAPAPAAKPEANGMKDKNGAVVRTLTMTNPAGYDVVLARKGLLDKQVELLSKALNSLSLTENTYGIYTGYNKFMPLSNELFEKLVKLQVQAESTENLVTEIDKIQKQN"
     primer_bind     complement(1586..1602)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1669..2257)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2428..3288)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3289..3393)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /label="MMSYN1_0708"
                     /note="high affinity transport system protein p37"
                     /translation="MYTNKLKVALGTMLSAVSIASVGSFVVACQTKEDWDTTITINNSWVNDGFFSKLDYDTGAVTPGDKSKAFIELLTKKFNELKNKDEATKKFKDVKFDIKVDFDKKTYFSKLEKNDSENDVYIANYSYYLSNVWNNKTKSLNKDLPFKLVSQAATLQFNWQSGDNTFYKDGKSTDDLRKLAEENNKKWLEFGEYPDWHKSDKAVNGKKLDFDGSKYTNFYKDVDLTYVYRGAVLIAGNEADREKIVKAWDDKNWDSFVKNGIVYEKTSSAGGYKYQVALFARHFGKTISEIKEDLEGKKYEQYIVKGQKVSAQLGKKQANSQLVPRIGFDDEGSYNWTKSEEGSEKYKPTDFKSTEKAMNGGKAMMTAAKPAAAKPAAAPAAPAPAAKPEANGMKDKNGAVVRTLTMTNPAGYDVVLARKGLLDKQVELLSKALNSLSLTENTYGIYTGYNKFMPLSNELFEKLVKLQVQAESTENLVTEIDKIQKQN"
ORIGIN
        1 ATGTATACGA ACAAGTTAAA AGTTGCGCTT GGTACCATGT TATCAGCAGT CTCTATTGCC
       61 AGTGTGGGCA GTTTTGTCGT CGCCTGTCAG ACGAAAGAAG ATTGGGATAC CACTATCACA
      121 ATCAACAACT CTTGGGTCAA CGATGGGTTC TTCAGCAAGC TTGACTACGA CACTGGGGCG
      181 GTCACCCCAG GAGATAAGTC CAAGGCATTC ATTGAACTTT TAACCAAAAA ATTTAACGAG
      241 TTGAAGAATA AGGACGAAGC TACGAAAAAG TTTAAGGACG TAAAATTCGA TATTAAAGTT
      301 GACTTTGACA AAAAGACTTA TTTTAGCAAA CTTGAGAAAA ACGACAGCGA AAATGACGTG
      361 TATATCGCAA ATTATTCTTA TTACCTGTCG AACGTATGGA ATAACAAAAC CAAGTCGCTG
      421 AACAAAGATC TGCCCTTCAA ACTGGTGTCG CAGGCAGCCA CGTTACAATT CAATTGGCAA
      481 AGCGGTGATA ATACCTTTTA CAAAGATGGT AAGTCTACTG ACGATTTGCG TAAACTTGCA
      541 GAAGAAAATA ATAAAAAATG GTTGGAGTTT GGAGAATATC CTGATTGGCA TAAATCCGAC
      601 AAAGCGGTCA ATGGTAAAAA ATTAGATTTC GATGGTTCTA AGTACACAAA TTTTTACAAG
      661 GACGTAGACC TGACTTATGT TTATCGTGGT GCCGTGTTAA TTGCGGGCAA TGAGGCAGAC
      721 CGCGAAAAAA TTGTAAAAGC ATGGGACGAT AAAAACTGGG ATTCATTCGT CAAAAACGGG
      781 ATCGTATACG AGAAAACCTC TTCTGCCGGT GGCTACAAGT ACCAGGTTGC CTTGTTTGCG
      841 CGTCACTTTG GAAAAACCAT TTCAGAGATC AAGGAGGACC TTGAGGGTAA AAAATACGAG
      901 CAATACATCG TCAAAGGACA AAAAGTCTCC GCGCAACTGG GCAAAAAGCA AGCTAATTCG
      961 CAATTAGTGC CCCGTATTGG CTTCGACGAC GAGGGGTCTT ACAATTGGAC AAAGAGTGAG
     1021 GAAGGATCTG AGAAGTACAA GCCAACTGAT TTCAAAAGTA CTGAAAAAGC TATGAACGGG
     1081 GGAAAGGCTA TGATGACCGC GGCAAAGCCT GCTGCGGCCA AACCCGCGGC TGCCCCTGCT
     1141 GCGCCAGCGC CCGCGGCAAA ACCGGAGGCA AATGGGATGA AGGATAAAAA TGGCGCTGTT
     1201 GTCCGCACCT TAACCATGAC CAACCCCGCT GGTTACGACG TGGTACTTGC TCGCAAAGGG
     1261 CTTCTGGACA AACAGGTGGA GCTTCTGTCA AAAGCATTGA ACTCGTTGTC ATTGACAGAG
     1321 AACACCTATG GAATTTATAC GGGTTACAAC AAATTCATGC CTTTGAGTAA CGAACTTTTC
     1381 GAAAAACTGG TTAAATTACA GGTCCAAGCG GAGTCGACGG AGAATCTTGT CACCGAGATT
     1441 GACAAAATCC AGAAGCAAAA CTGAAGAGCT TAGAGACCGG TACTAGTTCC TGCAGGGCTC
     1501 GAGGTGTCAA TCGTCGGAGC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA
     1561 CGGGTCTTGA GGGGTTTTTT GCATGGTCAT AGCTGTTTCC TGAGAGCTTG GCAGGTGATG
     1621 ACACACATTA ACAAATTTCG TGAGGAGTCT CCAGAAGAAT GCCATTAATT TCCATAGGCT
     1681 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC
     1741 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC
     1801 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC
     1861 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG
     1921 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA
     1981 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG
     2041 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA
     2101 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG
     2161 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG
     2221 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC
     2281 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC
     2341 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG
     2401 TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC
     2461 AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC
     2521 GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG AACCACGCTC
     2581 ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG
     2641 TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG
     2701 TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC
     2761 ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC
     2821 ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG
     2881 AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC
     2941 TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG
     3001 AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC
     3061 GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT
     3121 CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG
     3181 ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA
     3241 TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT
     3301 TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG
     3361 TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACCTGC ACCAGTCAGT AAAACGACGG
     3421 CCAGTAGTCA AAAGCCTCCG ACCGGAGGCT TTTGACTTGG TCAGGTGGAG TGAATTCGCG
     3481 GCCGCTTCTA GAGGCTCTTC GTAATTACGA AGAGGTCTCT A
//