LOCUS       Exported     2921 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2921
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      934..981
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2809..2825
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2826..2857
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /label="MMSYN1_0710"
                     /note=""
                     /translation="MYKIIAIDIDGTVYTRKNGIHELTKLAIKKAKDKGIKIVIATGRTITTTRFIAKQLDLLNTSIPFIGQNGGQVFSYEKNGSVKIRYTKNFTAQQVDQIFSIIKQHKAHAFCYTLNENIAYKNKGISIFFWWMKKRAQRVVKIYKPNKALESQITKYICFGKKENMRQMRKKIEDLGFSAFSFSYVTNAKENIEINPIGVNKGYGLEYVAKELNVKPEEILFFGDGENDLEAIKFAGKGVAMKNTKLDIVKNAADDITSLTADQGGVGEYIFKHVLKEEIPIEFQIDK"
     primer_bind     complement(986..1002)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1069..1657)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1828..2688)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2689..2793)
                     /note="ampicillin resistance gene promoter"
                     /label="bla promoter"
     CDS             complement(1..0)
                     /label="MMSYN1_0710"
                     /note=""
                     /translation="MYKIIAIDIDGTVYTRKNGIHELTKLAIKKAKDKGIKIVIATGRTITTTRFIAKQLDLLNTSIPFIGQNGGQVFSYEKNGSVKIRYTKNFTAQQVDQIFSIIKQHKAHAFCYTLNENIAYKNKGISIFFWWMKKRAQRVVKIYKPNKALESQITKYICFGKKENMRQMRKKIEDLGFSAFSFSYVTNAKENIEINPIGVNKGYGLEYVAKELNVKPEEILFFGDGENDLEAIKFAGKGVAMKNTKLDIVKNAADDITSLTADQGGVGEYIFKHVLKEEIPIEFQIDK"
ORIGIN
        1 ATGTACAAAA TTATCGCGAT TGACATTGAT GGTACTGTAT ACACCCGTAA AAACGGGATT
       61 CACGAACTTA CAAAACTGGC AATTAAAAAG GCTAAGGACA AGGGCATCAA AATTGTAATT
      121 GCGACAGGAC GCACCATCAC GACTACTCGC TTTATCGCCA AACAATTGGA CTTGTTGAAT
      181 ACTAGCATTC CATTTATCGG ACAAAATGGT GGACAGGTTT TCAGCTACGA GAAAAATGGT
      241 TCTGTAAAGA TTCGTTATAC TAAAAACTTT ACAGCCCAAC AGGTTGATCA AATCTTTAGT
      301 ATTATTAAGC AGCATAAGGC CCATGCCTTT TGTTACACAC TGAATGAGAA TATCGCGTAT
      361 AAGAACAAGG GTATCAGTAT CTTCTTTTGG TGGATGAAAA AACGCGCCCA GCGTGTGGTA
      421 AAGATTTATA AACCAAACAA AGCCTTAGAG TCTCAAATCA CCAAGTACAT CTGCTTTGGA
      481 AAGAAAGAGA ACATGCGTCA AATGCGTAAG AAAATCGAGG ATTTAGGGTT TAGTGCTTTT
      541 AGCTTCTCTT ACGTGACCAA CGCTAAGGAA AATATCGAAA TTAACCCTAT TGGGGTGAAT
      601 AAGGGATATG GGTTAGAATA TGTCGCTAAG GAGCTGAATG TAAAACCGGA GGAAATTCTT
      661 TTCTTCGGCG ACGGCGAGAA CGATCTTGAA GCAATTAAAT TTGCTGGTAA AGGCGTGGCT
      721 ATGAAGAACA CAAAATTAGA TATTGTAAAG AATGCTGCTG ATGATATTAC TTCCTTGACT
      781 GCTGACCAAG GGGGCGTAGG GGAGTATATT TTCAAGCATG TACTTAAAGA GGAAATTCCA
      841 ATCGAGTTTC AAATTGACAA GTGAAGAGCT TAGAGACCGG TACTAGTTCC TGCAGGGCTC
      901 GAGGTGTCAA TCGTCGGAGC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA
      961 CGGGTCTTGA GGGGTTTTTT GCATGGTCAT AGCTGTTTCC TGAGAGCTTG GCAGGTGATG
     1021 ACACACATTA ACAAATTTCG TGAGGAGTCT CCAGAAGAAT GCCATTAATT TCCATAGGCT
     1081 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC
     1141 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC
     1201 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC
     1261 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG
     1321 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA
     1381 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG
     1441 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA
     1501 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG
     1561 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG
     1621 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC
     1681 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC
     1741 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG
     1801 TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC
     1861 AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC
     1921 GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG AACCACGCTC
     1981 ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG
     2041 TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG
     2101 TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC
     2161 ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC
     2221 ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG
     2281 AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC
     2341 TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG
     2401 AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC
     2461 GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT
     2521 CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG
     2581 ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA
     2641 TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT
     2701 TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG
     2761 TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACCTGC ACCAGTCAGT AAAACGACGG
     2821 CCAGTAGTCA AAAGCCTCCG ACCGGAGGCT TTTGACTTGG TCAGGTGGAG TGAATTCGCG
     2881 GCCGCTTCTA GAGGCTCTTC GTAATTACGA AGAGGTCTCT A
//