LOCUS       Exported     3653 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3653
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1666..1713
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
                     /label="T7 terminator"
     primer_bind     3541..3557
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3558..3589
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /label="MMSYN1_0729"
                     /note="Catalyzes the interconversion of 2-phosphoglycerate and 3-phosphoglycerate."
                     /translation="MKVKRPVLLAILDGWGISEPDKGNAVDNANMVFVEYLKKTYPWLKAHASGKWVGLPDNQMGNSEVGHIHLGAGRINLESLAKLNHETKTNNIAKNEEIVKSFEYVKNNNSALHLMGLFSNGGVHSHFDHMIAIYKAAIDYGITNIKFDLITDGRDTKPKLAYDFVKDLLELIKQNNNIGVISSISGRYYAMDRDKRFDRSRIAYNAITNRNNVRSFTNILDYIQQEYMINHDDEMIIPAFNQDDLNGNLKANDAIIMTNFRPDRAIQISSILTNKNYIAWQSEAFSDAEFIGDKIRFVSMMKYSDSITSPHIAYPPKPLTNTLGQYLSKLGLKQLRIAETEKIAHVTFFFDGGNDYFKNGLAKNDEITLANAYIDLIPSAKVATYDLKPQMSAVEITDKLLEEIKKDEFDFIVLNFANCDMVGHTGNNKATEIACKTLDEQLKRIHEEFVLRHNGIMIITADHGNAEIMIDKDGQVNKKHTTSLVPIIITDLNIKLKQNDPAIAKVAPTILDLMNIEIPKEMELESMIDHN"
     primer_bind     complement(1718..1734)
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 rev"
     rep_origin      complement(1801..2389)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2560..3420)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3421..3525)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /translation="MKVKRPVLLAILDGWGISEPDKGNAVDNANMVFVEYLKKTYPWLKAHASGKWVGLPDNQMGNSEVGHIHLGAGRINLESLAKLNHETKTNNIAKNEEIVKSFEYVKNNNSALHLMGLFSNGGVHSHFDHMIAIYKAAIDYGITNIKFDLITDGRDTKPKLAYDFVKDLLELIKQNNNIGVISSISGRYYAMDRDKRFDRSRIAYNAITNRNNVRSFTNILDYIQQEYMINHDDEMIIPAFNQDDLNGNLKANDAIIMTNFRPDRAIQISSILTNKNYIAWQSEAFSDAEFIGDKIRFVSMMKYSDSITSPHIAYPPKPLTNTLGQYLSKLGLKQLRIAETEKIAHVTFFFDGGNDYFKNGLAKNDEITLANAYIDLIPSAKVATYDLKPQMSAVEITDKLLEEIKKDEFDFIVLNFANCDMVGHTGNNKATEIACKTLDEQLKRIHEEFVLRHNGIMIITADHGNAEIMIDKDGQVNKKHTTSLVPIIITDLNIKLKQNDPAIAKVAPTILDLMNIEIPKEMELESMIDHN"
                     /label="MMSYN1_0729"
                     /note="Catalyzes the interconversion of 2-phosphoglycerate and 3-phosphoglycerate."
ORIGIN
        1 ATGAAGGTAA AGCGTCCCGT ATTGCTGGCT ATCTTGGACG GATGGGGGAT CAGCGAACCT
       61 GACAAGGGAA ATGCGGTGGA CAATGCTAAC ATGGTGTTCG TAGAGTACCT GAAGAAAACC
      121 TATCCTTGGC TTAAAGCTCA TGCCTCCGGC AAGTGGGTTG GCTTACCAGA TAACCAAATG
      181 GGCAACTCCG AAGTGGGACA CATTCACCTG GGGGCTGGGC GCATTAACTT GGAGAGTCTG
      241 GCTAAATTGA ATCATGAAAC AAAGACTAAC AACATTGCTA AAAATGAGGA GATCGTCAAG
      301 TCATTTGAGT ACGTGAAGAA CAACAATTCA GCTCTGCATC TTATGGGATT ATTTAGCAAT
      361 GGCGGGGTTC ACTCTCACTT CGATCATATG ATTGCGATTT ATAAGGCAGC TATTGACTAT
      421 GGGATCACAA ACATTAAATT CGATTTGATT ACTGACGGAC GTGACACTAA GCCCAAGTTA
      481 GCGTATGACT TTGTAAAGGA TTTACTGGAA CTTATTAAAC AAAATAATAA TATTGGAGTC
      541 ATTTCTTCTA TTTCCGGGCG CTATTATGCC ATGGACCGCG ATAAACGTTT TGATCGCTCG
      601 CGCATTGCTT ATAACGCGAT TACAAATCGT AATAACGTTC GCTCGTTTAC CAATATCCTG
      661 GACTACATTC AGCAGGAGTA TATGATCAAC CACGATGACG AAATGATCAT CCCTGCATTC
      721 AATCAGGACG ACTTAAACGG AAATCTTAAG GCAAACGACG CCATCATCAT GACAAATTTT
      781 CGTCCAGATC GTGCTATTCA AATCTCCTCA ATCCTTACAA ATAAAAACTA TATTGCTTGG
      841 CAGTCGGAAG CGTTCTCAGA TGCAGAGTTC ATCGGGGATA AAATCCGCTT TGTATCTATG
      901 ATGAAGTACT CAGACTCTAT TACCAGCCCA CATATCGCTT ACCCTCCAAA ACCGCTGACG
      961 AATACACTGG GCCAATACTT ATCTAAGCTG GGACTGAAGC AATTACGCAT TGCGGAAACA
     1021 GAAAAAATCG CGCATGTAAC CTTCTTCTTT GACGGTGGGA ACGATTATTT CAAGAATGGC
     1081 TTAGCAAAAA ATGACGAAAT TACCTTGGCT AACGCGTACA TTGATTTGAT TCCGAGTGCT
     1141 AAGGTCGCTA CTTATGATTT AAAACCACAG ATGTCAGCGG TAGAAATTAC AGACAAACTG
     1201 TTGGAGGAAA TTAAGAAGGA CGAGTTCGAT TTCATCGTTC TGAATTTCGC TAATTGTGAT
     1261 ATGGTCGGAC ATACCGGGAA CAATAAGGCG ACAGAAATCG CCTGCAAGAC TTTGGACGAG
     1321 CAATTGAAAC GCATTCATGA AGAATTTGTC TTACGCCATA ATGGCATCAT GATCATTACC
     1381 GCAGACCACG GGAATGCAGA GATTATGATC GATAAAGACG GGCAAGTTAA CAAGAAACAC
     1441 ACAACAAGTT TAGTCCCAAT TATCATTACC GACCTTAATA TCAAATTAAA GCAAAACGAC
     1501 CCTGCGATTG CAAAAGTGGC CCCGACCATC TTGGACTTGA TGAACATCGA AATTCCTAAA
     1561 GAAATGGAGC TTGAGTCGAT GATTGACCAT AACTGAAGAG CTTAGAGACC GGTACTAGTT
     1621 CCTGCAGGGC TCGAGGTGTC AATCGTCGGA GCCGCTGAGC AATAACTAGC ATAACCCCTT
     1681 GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCATGGTC ATAGCTGTTT CCTGAGAGCT
     1741 TGGCAGGTGA TGACACACAT TAACAAATTT CGTGAGGAGT CTCCAGAAGA ATGCCATTAA
     1801 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG
     1861 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG
     1921 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG
     1981 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC
     2041 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA
     2101 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG
     2161 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC
     2221 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC
     2281 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG
     2341 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT
     2401 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT
     2461 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA
     2521 ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA
     2581 GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT
     2641 GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG
     2701 AGAACCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA
     2761 GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA
     2821 AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG
     2881 CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC
     2941 AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC
     3001 GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA
     3061 TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC
     3121 CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG
     3181 GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC
     3241 GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG
     3301 TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC
     3361 AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT
     3421 ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA
     3481 CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACCT GCACCAGTCA
     3541 GTAAAACGAC GGCCAGTAGT CAAAAGCCTC CGACCGGAGG CTTTTGACTT GGTCAGGTGG
     3601 AGTGAATTCG CGGCCGCTTC TAGAGGCTCT TCGTAATTAC GAAGAGGTCT CTA
//