LOCUS Exported 2726 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..2726 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 739..786 /note="transcription terminator for bacteriophage T7 RNA polymerase" /label="T7 terminator" primer_bind 2614..2630 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 2631..2662 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 1..0 /note="Catalyzes a reversible aldol reaction between acetaldehyde and D-glyceraldehyde 3-phosphate to generate 2-deoxy-D-ribose 5-phosphate." /translation="MEIKLNKYIDHTLLKPEATKQDIINLCNQAIQYDFATVCVNTCWTSLCKELLKNSNVGITNVVGFPLGACLTEVKVFETKKAIENGCDEIDMVLNIGALKDKDYDLVLNDMKEVKKAANDHVVKVILENCLLTEQEIIKACELAVKAGIDFVKTSTGFNKSGANIKDVKLMSEVVKNKAKVKAAGGVRTYDDAIAMINAGASRLGTSGSVEIVLKQENKSNY" /label="MMSYN1_0732" primer_bind complement(791..807) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(874..1462) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(1633..2493) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(2494..2598) /label="bla promoter" /note="ampicillin resistance gene promoter" CDS complement(1..0) /label="MMSYN1_0732" /note="Catalyzes a reversible aldol reaction between acetaldehyde and D-glyceraldehyde 3-phosphate to generate 2-deoxy-D-ribose 5-phosphate." /translation="MEIKLNKYIDHTLLKPEATKQDIINLCNQAIQYDFATVCVNTCWTSLCKELLKNSNVGITNVVGFPLGACLTEVKVFETKKAIENGCDEIDMVLNIGALKDKDYDLVLNDMKEVKKAANDHVVKVILENCLLTEQEIIKACELAVKAGIDFVKTSTGFNKSGANIKDVKLMSEVVKNKAKVKAAGGVRTYDDAIAMINAGASRLGTSGSVEIVLKQENKSNY" ORIGIN 1 ATGGAGATTA AGCTGAATAA ATACATCGAC CACACATTGT TAAAACCAGA GGCAACAAAA 61 CAAGACATTA TTAACTTATG CAATCAGGCA ATCCAGTACG ACTTTGCCAC TGTCTGCGTC 121 AACACGTGCT GGACCTCACT GTGCAAAGAG CTTTTAAAAA ACTCAAATGT CGGAATCACT 181 AATGTCGTTG GTTTCCCTCT TGGCGCGTGC CTGACCGAGG TGAAGGTGTT TGAAACGAAG 241 AAAGCGATTG AGAATGGTTG CGACGAAATT GACATGGTAT TAAATATCGG CGCTTTAAAG 301 GACAAAGACT ATGATCTTGT ACTTAACGAT ATGAAGGAAG TGAAAAAGGC CGCAAATGAC 361 CATGTGGTAA AGGTGATTTT AGAAAACTGC CTGTTGACGG AGCAGGAAAT TATCAAAGCT 421 TGTGAACTTG CTGTGAAAGC AGGAATCGAT TTTGTTAAAA CATCAACGGG CTTTAACAAA 481 TCAGGTGCAA ATATTAAGGA TGTAAAACTG ATGTCGGAAG TAGTAAAAAA CAAAGCTAAA 541 GTAAAGGCCG CTGGGGGAGT TCGTACCTAC GATGACGCTA TTGCAATGAT TAACGCAGGC 601 GCTTCTCGTC TTGGAACCTC GGGTTCCGTT GAAATTGTTC TTAAACAAGA AAATAAGAGT 661 AACTACTGAA GAGCTTAGAG ACCGGTACTA GTTCCTGCAG GGCTCGAGGT GTCAATCGTC 721 GGAGCCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT 781 TTTTTGCATG GTCATAGCTG TTTCCTGAGA GCTTGGCAGG TGATGACACA CATTAACAAA 841 TTTCGTGAGG AGTCTCCAGA AGAATGCCAT TAATTTCCAT AGGCTCCGCC CCCCTGACGA 901 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 961 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 1021 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 1081 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 1141 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 1201 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 1261 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 1321 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 1381 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 1441 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 1501 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 1561 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC 1621 TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT 1681 TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT 1741 ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGAACCA CGCTCACCGG CTCCAGATTT 1801 ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC 1861 CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA 1921 TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG 1981 TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT 2041 GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC 2101 AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT 2161 AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG 2221 GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC 2281 TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC 2341 GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT 2401 TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG 2461 AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG 2521 CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA 2581 ACAAATAGGG GTTCCGCGCA CCTGCACCAG TCAGTAAAAC GACGGCCAGT AGTCAAAAGC 2641 CTCCGACCGG AGGCTTTTGA CTTGGTCAGG TGGAGTGAAT TCGCGGCCGC TTCTAGAGGC 2701 TCTTCGTAAT TACGAAGAGG TCTCTA //