LOCUS       Exported     3734 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3734
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1747..1794
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3622..3638
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 for"
     terminator      3639..3670
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /label="MMSYN1_0733"
                     /note=""
                     /translation="MSFNKLNQTYLDWINHPNLDQELKELLNKADDNELNAAFNLELKFGTAGIRGILGAGPGRFNVYTIKKVTIAYAKLLQTKYSNDLNKGVVIGHDNRHNSKKFAKLVADILTSFNIKAYLFKNNDLQPTPVVSFATKALNCIGGIVITASHNPAEYNGYKIYDPYGCQLMPHDTDVIANYMNEITNILDWTFISNNNLLEIVDQTVIDKYFEMIKNLEFYKDQDKSNLKIIYSAVNGTGSLYTPIVLKQSGYEVIEVKEHAFEDETFKNVINPNPEFDPAWKIPLEYAKKYDADIIILNDPDADRFGMAIKHNNEFIRLNGNQTGAILIDWKLSNLKRLNKLPKNPALYSSFVTSDLGDRIASETYNANVVKTLTGFKWMGQEMLKEPLNGLNFVFAYEESYGYVIDDSTRDKDGIQASIIAAEACWYYKNQNMTLVDYLNQLYEKYGYYYTTTYNLNFKPEEKDSKIAPIMKLLRTTGIKQINNLKVVKIEDYINGLYNMPSEDLLKIYLEDKSWIAIRPSGTEPKLKIYFVIVDSSLQKAENKAEKIYTELKTILNI"
     primer_bind     complement(1799..1815)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1882..2470)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2641..3501)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3502..3606)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /label="MMSYN1_0733"
                     /note=""
                     /translation="MSFNKLNQTYLDWINHPNLDQELKELLNKADDNELNAAFNLELKFGTAGIRGILGAGPGRFNVYTIKKVTIAYAKLLQTKYSNDLNKGVVIGHDNRHNSKKFAKLVADILTSFNIKAYLFKNNDLQPTPVVSFATKALNCIGGIVITASHNPAEYNGYKIYDPYGCQLMPHDTDVIANYMNEITNILDWTFISNNNLLEIVDQTVIDKYFEMIKNLEFYKDQDKSNLKIIYSAVNGTGSLYTPIVLKQSGYEVIEVKEHAFEDETFKNVINPNPEFDPAWKIPLEYAKKYDADIIILNDPDADRFGMAIKHNNEFIRLNGNQTGAILIDWKLSNLKRLNKLPKNPALYSSFVTSDLGDRIASETYNANVVKTLTGFKWMGQEMLKEPLNGLNFVFAYEESYGYVIDDSTRDKDGIQASIIAAEACWYYKNQNMTLVDYLNQLYEKYGYYYTTTYNLNFKPEEKDSKIAPIMKLLRTTGIKQINNLKVVKIEDYINGLYNMPSEDLLKIYLEDKSWIAIRPSGTEPKLKIYFVIVDSSLQKAENKAEKIYTELKTILNI"
ORIGIN
        1 ATGTCTTTCA ATAAGCTGAA TCAAACATAC CTTGATTGGA TTAACCACCC CAACTTAGAC
       61 CAGGAATTAA AGGAGCTGCT GAATAAGGCT GATGACAACG AACTGAACGC GGCTTTTAAC
      121 CTGGAACTGA AATTTGGAAC TGCGGGTATC CGCGGTATTT TGGGCGCGGG TCCGGGGCGT
      181 TTCAATGTGT ACACTATTAA GAAGGTTACT ATCGCTTACG CCAAGCTTTT ACAGACGAAG
      241 TACAGCAATG ACCTTAATAA AGGGGTGGTG ATCGGGCACG ACAACCGCCA TAACAGTAAA
      301 AAGTTTGCTA AACTGGTGGC AGACATCCTG ACTTCGTTCA ACATCAAGGC CTATCTGTTC
      361 AAGAACAACG ATTTACAACC GACCCCGGTT GTATCGTTTG CGACCAAGGC CTTAAATTGT
      421 ATTGGTGGCA TCGTTATTAC CGCTTCTCAC AACCCCGCGG AGTATAATGG ATATAAGATT
      481 TACGACCCCT ATGGTTGCCA ATTGATGCCT CATGACACTG ATGTCATTGC GAACTACATG
      541 AATGAAATTA CTAACATCCT GGACTGGACG TTTATCAGTA ACAATAATTT GTTGGAGATC
      601 GTCGACCAAA CGGTGATTGA TAAGTATTTT GAGATGATTA AGAATTTGGA ATTTTACAAG
      661 GATCAGGACA AATCAAATCT GAAAATCATT TACTCAGCTG TGAACGGGAC GGGGTCATTA
      721 TATACTCCTA TCGTACTTAA ACAAAGTGGC TATGAAGTCA TCGAGGTTAA GGAGCACGCA
      781 TTCGAAGATG AAACGTTTAA AAACGTCATC AACCCTAATC CAGAATTCGA CCCCGCCTGG
      841 AAAATCCCAT TAGAGTATGC TAAGAAATAC GATGCAGACA TCATTATTTT GAACGATCCC
      901 GATGCGGATC GTTTCGGGAT GGCTATCAAA CACAATAACG AATTTATTCG TTTGAACGGG
      961 AACCAGACGG GGGCAATCTT AATCGATTGG AAATTATCAA ATCTGAAACG TTTGAATAAA
     1021 CTTCCGAAAA ATCCTGCGTT ATATAGTAGC TTTGTAACCA GTGACCTGGG AGATCGTATC
     1081 GCCAGTGAAA CGTATAACGC CAATGTTGTC AAGACCTTGA CAGGGTTTAA GTGGATGGGA
     1141 CAGGAGATGT TAAAGGAACC GCTTAATGGG TTAAACTTCG TGTTCGCCTA CGAAGAGTCG
     1201 TACGGGTATG TTATTGATGA TTCGACTCGT GATAAAGACG GTATTCAGGC GAGCATTATT
     1261 GCTGCAGAGG CCTGTTGGTA TTACAAAAAT CAAAATATGA CGCTGGTTGA CTATCTGAAT
     1321 CAACTTTATG AAAAGTACGG ATATTACTAT ACGACGACTT ACAACCTGAA CTTCAAACCC
     1381 GAGGAGAAAG ATTCCAAGAT CGCCCCTATT ATGAAACTTC TTCGCACAAC CGGTATCAAG
     1441 CAAATTAACA ATCTGAAAGT AGTAAAAATT GAAGATTACA TTAATGGCCT TTATAATATG
     1501 CCGAGCGAGG ATCTGCTGAA AATCTATTTA GAGGACAAGA GTTGGATCGC AATTCGTCCA
     1561 AGTGGGACGG AACCCAAATT AAAGATTTAT TTTGTAATCG TAGACAGTTC ATTGCAAAAG
     1621 GCTGAAAATA AGGCGGAAAA AATTTATACC GAGCTCAAGA CAATTCTGAA TATTTGAAGA
     1681 GCTTAGAGAC CGGTACTAGT TCCTGCAGGG CTCGAGGTGT CAATCGTCGG AGCCGCTGAG
     1741 CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCATGGT
     1801 CATAGCTGTT TCCTGAGAGC TTGGCAGGTG ATGACACACA TTAACAAATT TCGTGAGGAG
     1861 TCTCCAGAAG AATGCCATTA ATTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA
     1921 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC
     1981 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC
     2041 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG
     2101 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA
     2161 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC
     2221 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC
     2281 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG
     2341 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA
     2401 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA
     2461 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA
     2521 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT
     2581 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG
     2641 TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT
     2701 AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC
     2761 CAGTGCTGCA ATGATACCGC GAGAACCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA
     2821 CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA
     2881 GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA
     2941 CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT
     3001 CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC
     3061 GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT
     3121 CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC
     3181 TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG
     3241 CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT
     3301 CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC
     3361 CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG
     3421 CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC
     3481 ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG
     3541 TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT
     3601 TCCGCGCACC TGCACCAGTC AGTAAAACGA CGGCCAGTAG TCAAAAGCCT CCGACCGGAG
     3661 GCTTTTGACT TGGTCAGGTG GAGTGAATTC GCGGCCGCTT CTAGAGGCTC TTCGTAATTA
     3721 CGAAGAGGTC TCTA
//