LOCUS Exported 3077 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..3077 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 1090..1137 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 2965..2981 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 2982..3013 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 1..0 /label="MMSYN1_0773" /note="" /translation="MAKEQKYYHESVSPIEFVKNNFKGNLRSVNWNVINDEKDLEVWNRITQNFWLPEKIPVSNDLSSWRSLSSEWQQLVTRTFTGLTLLDTVQATVGDVAQIEHSLTDHEQVIYSNFAFMVGVHARSYGTIFSTLCSSEQIEEAHEWVVKTETLQKRAKALIPYYTGTDPLKSKVAAALMPGFLLYGGFYLPFYLSARGKLPNTSDIIRLILRDKVIHNYYSGYKYQKKVAKLPVEKQAEMKEFVFKLLYELIDLETAYLKELYAGFDIVDDAIRFSVYNAGKFLQNLGYDSPFSEEETRIEPEIFNQLSARADENHDFFSGNGSSYVMGVSVETEDEDWEF" primer_bind complement(1142..1158) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(1225..1813) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(1984..2844) /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" promoter complement(2845..2949) /label="bla promoter" /note="ampicillin resistance gene promoter" CDS complement(1..0) /label="MMSYN1_0773" /note="" /translation="MAKEQKYYHESVSPIEFVKNNFKGNLRSVNWNVINDEKDLEVWNRITQNFWLPEKIPVSNDLSSWRSLSSEWQQLVTRTFTGLTLLDTVQATVGDVAQIEHSLTDHEQVIYSNFAFMVGVHARSYGTIFSTLCSSEQIEEAHEWVVKTETLQKRAKALIPYYTGTDPLKSKVAAALMPGFLLYGGFYLPFYLSARGKLPNTSDIIRLILRDKVIHNYYSGYKYQKKVAKLPVEKQAEMKEFVFKLLYELIDLETAYLKELYAGFDIVDDAIRFSVYNAGKFLQNLGYDSPFSEEETRIEPEIFNQLSARADENHDFFSGNGSSYVMGVSVETEDEDWEF" ORIGIN 1 ATGGCAAAGG AGCAAAAATA CTACCATGAG TCAGTGTCAC CTATTGAGTT TGTCAAAAAT 61 AATTTTAAAG GGAATCTGCG CAGTGTGAAT TGGAATGTAA TTAATGACGA GAAGGATCTT 121 GAGGTGTGGA ACCGCATCAC TCAGAACTTC TGGTTGCCCG AAAAGATTCC TGTAAGCAAC 181 GATTTATCTA GCTGGCGTTC GCTGAGTTCT GAGTGGCAGC AACTGGTAAC ACGCACTTTC 241 ACTGGGTTGA CGCTTTTAGA CACAGTCCAA GCCACCGTAG GCGACGTTGC CCAGATCGAA 301 CACAGTCTTA CGGACCACGA ACAGGTAATC TATTCGAACT TTGCTTTTAT GGTTGGCGTG 361 CACGCACGCT CCTATGGGAC GATTTTCTCC ACTTTATGCT CGTCGGAACA GATTGAGGAG 421 GCTCACGAGT GGGTTGTTAA GACTGAAACG TTGCAGAAAC GTGCTAAAGC GCTTATTCCC 481 TACTACACGG GCACGGACCC GCTTAAATCT AAAGTTGCAG CCGCGCTTAT GCCCGGATTT 541 CTGTTGTACG GAGGCTTTTA TTTACCTTTT TATTTAAGCG CTCGCGGTAA ATTGCCTAAC 601 ACCTCTGATA TTATTCGCTT GATCTTGCGT GATAAAGTTA TTCACAATTA CTACTCCGGG 661 TACAAGTACC AGAAGAAGGT TGCAAAACTT CCAGTAGAGA AACAGGCCGA AATGAAAGAG 721 TTCGTTTTCA AGCTTCTTTA CGAACTGATT GACTTGGAAA CGGCTTATTT AAAAGAGTTG 781 TACGCTGGGT TCGATATTGT CGATGATGCC ATCCGCTTCT CCGTATATAA TGCTGGCAAG 841 TTCCTGCAGA ATCTGGGCTA TGATTCACCA TTTAGCGAGG AGGAAACCCG TATTGAACCA 901 GAAATCTTTA ATCAATTAAG CGCTCGTGCA GATGAAAACC ACGACTTCTT TAGCGGTAAC 961 GGTTCTTCTT ATGTGATGGG AGTATCCGTC GAGACAGAGG ACGAGGACTG GGAGTTTTGA 1021 AGAGCTTAGA GACCGGTACT AGTTCCTGCA GGGCTCGAGG TGTCAATCGT CGGAGCCGCT 1081 GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCAT 1141 GGTCATAGCT GTTTCCTGAG AGCTTGGCAG GTGATGACAC ACATTAACAA ATTTCGTGAG 1201 GAGTCTCCAG AAGAATGCCA TTAATTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 1261 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 1321 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 1381 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 1441 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 1501 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 1561 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 1621 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 1681 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 1741 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 1801 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 1861 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 1921 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA 1981 CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC 2041 CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG 2101 CCCCAGTGCT GCAATGATAC CGCGAGAACC ACGCTCACCG GCTCCAGATT TATCAGCAAT 2161 AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT 2221 CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG 2281 CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC 2341 ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA 2401 AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC 2461 ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT 2521 TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG 2581 TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT 2641 GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG 2701 ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC 2761 CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC 2821 GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA 2881 GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG 2941 GGTTCCGCGC ACCTGCACCA GTCAGTAAAA CGACGGCCAG TAGTCAAAAG CCTCCGACCG 3001 GAGGCTTTTG ACTTGGTCAG GTGGAGTGAA TTCGCGGCCG CTTCTAGAGG CTCTTCGTAA 3061 TTACGAAGAG GTCTCTA //