LOCUS       Exported     3077 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3077
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1090..1137
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2965..2981
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2982..3013
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /label="MMSYN1_0773"
                     /note=""
                     /translation="MAKEQKYYHESVSPIEFVKNNFKGNLRSVNWNVINDEKDLEVWNRITQNFWLPEKIPVSNDLSSWRSLSSEWQQLVTRTFTGLTLLDTVQATVGDVAQIEHSLTDHEQVIYSNFAFMVGVHARSYGTIFSTLCSSEQIEEAHEWVVKTETLQKRAKALIPYYTGTDPLKSKVAAALMPGFLLYGGFYLPFYLSARGKLPNTSDIIRLILRDKVIHNYYSGYKYQKKVAKLPVEKQAEMKEFVFKLLYELIDLETAYLKELYAGFDIVDDAIRFSVYNAGKFLQNLGYDSPFSEEETRIEPEIFNQLSARADENHDFFSGNGSSYVMGVSVETEDEDWEF"
     primer_bind     complement(1142..1158)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1225..1813)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1984..2844)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
     promoter        complement(2845..2949)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /label="MMSYN1_0773"
                     /note=""
                     /translation="MAKEQKYYHESVSPIEFVKNNFKGNLRSVNWNVINDEKDLEVWNRITQNFWLPEKIPVSNDLSSWRSLSSEWQQLVTRTFTGLTLLDTVQATVGDVAQIEHSLTDHEQVIYSNFAFMVGVHARSYGTIFSTLCSSEQIEEAHEWVVKTETLQKRAKALIPYYTGTDPLKSKVAAALMPGFLLYGGFYLPFYLSARGKLPNTSDIIRLILRDKVIHNYYSGYKYQKKVAKLPVEKQAEMKEFVFKLLYELIDLETAYLKELYAGFDIVDDAIRFSVYNAGKFLQNLGYDSPFSEEETRIEPEIFNQLSARADENHDFFSGNGSSYVMGVSVETEDEDWEF"
ORIGIN
        1 ATGGCAAAGG AGCAAAAATA CTACCATGAG TCAGTGTCAC CTATTGAGTT TGTCAAAAAT
       61 AATTTTAAAG GGAATCTGCG CAGTGTGAAT TGGAATGTAA TTAATGACGA GAAGGATCTT
      121 GAGGTGTGGA ACCGCATCAC TCAGAACTTC TGGTTGCCCG AAAAGATTCC TGTAAGCAAC
      181 GATTTATCTA GCTGGCGTTC GCTGAGTTCT GAGTGGCAGC AACTGGTAAC ACGCACTTTC
      241 ACTGGGTTGA CGCTTTTAGA CACAGTCCAA GCCACCGTAG GCGACGTTGC CCAGATCGAA
      301 CACAGTCTTA CGGACCACGA ACAGGTAATC TATTCGAACT TTGCTTTTAT GGTTGGCGTG
      361 CACGCACGCT CCTATGGGAC GATTTTCTCC ACTTTATGCT CGTCGGAACA GATTGAGGAG
      421 GCTCACGAGT GGGTTGTTAA GACTGAAACG TTGCAGAAAC GTGCTAAAGC GCTTATTCCC
      481 TACTACACGG GCACGGACCC GCTTAAATCT AAAGTTGCAG CCGCGCTTAT GCCCGGATTT
      541 CTGTTGTACG GAGGCTTTTA TTTACCTTTT TATTTAAGCG CTCGCGGTAA ATTGCCTAAC
      601 ACCTCTGATA TTATTCGCTT GATCTTGCGT GATAAAGTTA TTCACAATTA CTACTCCGGG
      661 TACAAGTACC AGAAGAAGGT TGCAAAACTT CCAGTAGAGA AACAGGCCGA AATGAAAGAG
      721 TTCGTTTTCA AGCTTCTTTA CGAACTGATT GACTTGGAAA CGGCTTATTT AAAAGAGTTG
      781 TACGCTGGGT TCGATATTGT CGATGATGCC ATCCGCTTCT CCGTATATAA TGCTGGCAAG
      841 TTCCTGCAGA ATCTGGGCTA TGATTCACCA TTTAGCGAGG AGGAAACCCG TATTGAACCA
      901 GAAATCTTTA ATCAATTAAG CGCTCGTGCA GATGAAAACC ACGACTTCTT TAGCGGTAAC
      961 GGTTCTTCTT ATGTGATGGG AGTATCCGTC GAGACAGAGG ACGAGGACTG GGAGTTTTGA
     1021 AGAGCTTAGA GACCGGTACT AGTTCCTGCA GGGCTCGAGG TGTCAATCGT CGGAGCCGCT
     1081 GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCAT
     1141 GGTCATAGCT GTTTCCTGAG AGCTTGGCAG GTGATGACAC ACATTAACAA ATTTCGTGAG
     1201 GAGTCTCCAG AAGAATGCCA TTAATTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA
     1261 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT
     1321 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT
     1381 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT
     1441 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC
     1501 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT
     1561 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC
     1621 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT
     1681 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA
     1741 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA
     1801 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA
     1861 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT
     1921 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA
     1981 CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC
     2041 CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG
     2101 CCCCAGTGCT GCAATGATAC CGCGAGAACC ACGCTCACCG GCTCCAGATT TATCAGCAAT
     2161 AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT
     2221 CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG
     2281 CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC
     2341 ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA
     2401 AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC
     2461 ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT
     2521 TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG
     2581 TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT
     2641 GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG
     2701 ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC
     2761 CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC
     2821 GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA
     2881 GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG
     2941 GGTTCCGCGC ACCTGCACCA GTCAGTAAAA CGACGGCCAG TAGTCAAAAG CCTCCGACCG
     3001 GAGGCTTTTG ACTTGGTCAG GTGGAGTGAA TTCGCGGCCG CTTCTAGAGG CTCTTCGTAA
     3061 TTACGAAGAG GTCTCTA
//