LOCUS       Exported     4138 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4138
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2010..4124
                     /note="3'-5' exoribonuclease that releases 5'-nucleoside monophosphates and is involved in maturation of structured RNAs."
                     /translation="MESKIIEILKKNHHKISLNKLLTYFKALDYSLVKNYLDQLEKNNNIAISLENNIYLLDEIYKKGTIKLNPKGFGFINDVNDLTTEDHFIAGVDLNNSIHQDEVVYILKQEEDNRLKAIVIDLIKRNKVYLIGEINRSFDKRFLDFIPNDKSFDSFRFVIVNKNEFKYEEFNIIKAKIISCKERKIFIRLIKIIGNSKKASDRILAIAEEFDIKTSFDKQTLDNAKQINLSTDKLEKEFLKRQNNSLVNKTIVTIDGIDSKDLDDAICVEKLENNNYKLFVAIADVSYFVRYKTSLDKEALLRGNSTYLANKVIPMLPNILSDDLCSLNPNTKKLVFVCEMEFDNNANMLNKKVYESVIISKARLNYDEVNNYFKTKTWTHDDKTKKMLDIAYELYKKLEDLKAKKGTISFDVREPKIILDKNLNVIDIKTKTADQAEKLIEQFMVSCNEAVAELIYQKDLPFLYRNHNKPDEDELINWYKSLKTFGINPKLTNKQVLDPIFINHTLSQIKEQIKDETEVELLNISLLRYMDKAKYGLENIGHFGLASDCYTHFTSPIRRYSDLLVHRYLKQYLITKDLEKTSLENNTNYVNKVSNIINDTETKSVECEREVIKACMCEYMLNKVNNTYTATISAVLKFGIFIQLDNLVEGLVHISNMNSDLVYDETNRILIKPDNTYYRMGQKVKVKLINVDIKKRTIDFVLIE"
                     /label="MMSYN1_0775"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GGGTCTCAAA TGGAAAGCAA AATTATTGAA ATTCTGAAGA
     2041 AAAACCATCA TAAAATTAGC CTGAACAAAC TGCTGACCTA TTTTAAAGCG CTGGATTATA
     2101 GCCTGGTGAA AAACTATCTG GATCAGCTGG AGAAAAACAA CAACATTGCG ATTAGCCTGG
     2161 AAAACAACAT TTATCTGCTG GATGAAATTT ATAAGAAAGG CACCATTAAA CTGAACCCGA
     2221 AAGGCTTTGG CTTTATTAAC GATGTGAACG ATCTGACCAC CGAAGATCAT TTTATTGCGG
     2281 GCGTGGATCT GAACAACAGC ATTCATCAGG ATGAAGTGGT GTATATTCTG AAACAGGAAG
     2341 AAGATAACCG CCTGAAAGCG ATTGTGATTG ATCTGATTAA ACGCAACAAA GTGTATCTGA
     2401 TTGGCGAAAT TAACCGCAGC TTTGATAAAC GCTTTCTGGA TTTTATTCCG AACGATAAAA
     2461 GCTTTGATAG CTTTCGCTTT GTGATTGTGA ACAAAAACGA ATTTAAATAT GAAGAATTTA
     2521 ACATTATTAA AGCGAAAATT ATTAGCTGCA AAGAACGCAA AATTTTTATT CGCCTGATTA
     2581 AAATTATTGG CAACAGCAAG AAAGCGAGCG ATCGCATTCT GGCGATTGCG GAAGAATTTG
     2641 ATATTAAAAC CAGCTTTGAT AAACAGACCC TGGATAACGC GAAACAGATT AACCTGAGCA
     2701 CCGATAAACT GGAAAAAGAA TTTCTGAAAC GCCAGAACAA CAGCCTGGTG AACAAAACCA
     2761 TTGTGACCAT TGATGGCATT GATAGCAAAG ATCTGGATGA TGCGATTTGC GTGGAAAAAC
     2821 TGGAAAACAA CAACTATAAA CTGTTTGTGG CGATTGCGGA TGTGAGCTAT TTTGTGCGCT
     2881 ATAAAACCAG CCTGGATAAA GAAGCGCTGC TGCGCGGCAA CAGCACCTAT CTGGCGAACA
     2941 AAGTGATTCC GATGCTGCCG AACATTCTGT CCGATGATCT GTGCAGCCTG AACCCGAACA
     3001 CCAAGAAACT GGTGTTTGTG TGCGAAATGG AATTTGATAA CAACGCGAAC ATGCTGAACA
     3061 AGAAAGTGTA TGAAAGCGTG ATTATTAGCA AAGCGCGCCT GAACTATGAT GAAGTGAACA
     3121 ACTATTTTAA AACCAAAACC TGGACCCATG ATGATAAAAC CAAGAAAATG CTGGATATTG
     3181 CGTATGAACT GTATAAAAAA CTGGAAGATC TGAAAGCGAA AAAAGGCACC ATTAGCTTTG
     3241 ATGTGCGCGA ACCGAAAATT ATTCTGGATA AAAACCTGAA CGTGATTGAT ATTAAAACCA
     3301 AAACCGCGGA TCAGGCGGAA AAACTGATTG AACAGTTTAT GGTGAGCTGC AACGAAGCGG
     3361 TGGCGGAACT GATTTATCAG AAAGATCTGC CGTTTCTGTA TCGCAACCAT AACAAACCGG
     3421 ATGAAGATGA ACTGATTAAC TGGTATAAAA GCCTGAAAAC CTTTGGCATT AACCCGAAAC
     3481 TGACCAACAA ACAGGTGCTG GATCCGATTT TTATTAACCA TACCCTGAGC CAGATTAAAG
     3541 AACAGATTAA AGATGAAACC GAAGTGGAAC TGCTGAACAT TAGCCTGCTG CGCTATATGG
     3601 ATAAAGCGAA ATATGGCCTG GAAAACATTG GCCATTTTGG CCTGGCGAGC GATTGCTATA
     3661 CCCATTTTAC CAGCCCGATT CGCCGCTATA GCGATCTGCT GGTGCACCGC TATCTGAAAC
     3721 AGTATCTGAT TACCAAAGAT CTGGAAAAAA CCAGCCTGGA AAACAACACC AACTATGTGA
     3781 ACAAAGTGAG CAACATTATT AACGATACCG AAACCAAAAG CGTGGAATGC GAACGCGAAG
     3841 TGATTAAAGC GTGCATGTGC GAATATATGC TGAACAAAGT GAACAACACC TATACCGCGA
     3901 CCATTAGCGC GGTGCTGAAA TTTGGCATTT TTATTCAGCT GGATAACCTG GTGGAAGGCC
     3961 TGGTGCATAT TAGCAACATG AACAGCGATC TGGTGTATGA TGAAACCAAC CGCATTCTGA
     4021 TTAAACCGGA TAACACCTAT TATCGCATGG GCCAGAAAGT GAAAGTGAAA CTGATTAACG
     4081 TGGATATTAA AAAACGCACC ATTGATTTTG TGCTGATTGA GTGAAGAGCT TAGAGACC
//