LOCUS       Exported     4261 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4261
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
                     /label="tonB terminator"
     CDS             2010..4247
                     /label="MMSYN1_0779"
                     /note="PtsG: PTS system glucose-specific EIICB component"
                     /translation="MLTQTNKSSFKDKLKAFGANIMPTLSKLSKAFLLPIALLPIAGVFLGVGAAIAANTPEQSALWFIGKVMGNMGDVCFGNLPVLFCISVALAYTKDSGVAAITAVVGFLVFNGVQAPLFTQGQVQSDKVSEYSLLWYKHVSNSLTGSNMGILSLNTGVLGGIFVGAIAAKCYNKFHQTQLPTAISFFSGTKLVPIITFVAVIPLSFIFMMAWPVIGLGLNKFGQVSGTLPYGTDSLIFEIVERSLVPFGLHHVFYAPLWWTSAGGSIAEGFNNLSKQSEAIQKVFVDSYNSLHGTHHTKLQEIIDIVQKNKELWGAVGDQIISQRVIGHLNILNFTDVEKLGINLGRFQSGKFGFMLLGLPAAALAMWLAAPKENRQQVFGIYFSAAFTCFLTGITEPIEYTFLFVAPWLFYGVHMPLASIAFWATGALQTHITQTVSGGIIDYIVFGIIPFISGAMKPISAFGVLGVAVVLAPIYFCAFYFLIKLFNVKTPGRDGNAEAKLYTKADFKASKGLNVDGSKMSSSTDDKEQARLAKAAAIIEYLGGEENIVDVDSCASRLRLTVVDAKKADIDGIKSLGGTTGALVKGNNIQIVYGGEQEAIKPRMQKLLEQQRQEKMMGHSEMKSEEMKSENMSMTCESNQACDKKDEACGCEKDCMCEEPNKDEEQPVSEMKVEEKVVDEMKVEKPKQTRTKSTKAKSTTSKSTKSTSKSSSKSTKSTSSKAKSTSSKPKTTKAKSTSSKTKTTK"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GGGTCTCAAA TGCTTACCCA GACGAATAAA TCTAGTTTCA
     2041 AAGATAAGTT AAAGGCCTTC GGAGCCAACA TAATGCCTAC ACTGAGTAAG CTGTCAAAAG
     2101 CTTTCTTGCT GCCGATCGCT TTATTGCCAA TAGCCGGTGT GTTTTTAGGT GTCGGAGCTG
     2161 CAATAGCGGC GAATACGCCG GAGCAGTCTG CATTGTGGTT TATCGGTAAG GTAATGGGCA
     2221 ATATGGGGGA CGTTTGCTTC GGTAATTTGC CAGTGTTGTT TTGTATTTCT GTTGCTTTAG
     2281 CCTATACCAA GGACTCGGGC GTAGCTGCTA TAACCGCCGT GGTCGGATTC TTGGTTTTCA
     2341 ATGGGGTCCA AGCGCCATTA TTCACACAGG GTCAGGTACA ATCGGATAAG GTTTCTGAAT
     2401 ATAGCCTGCT GTGGTATAAG CATGTAAGTA ATTCTCTTAC AGGTTCAAAC ATGGGTATAC
     2461 TTTCGTTAAA CACCGGCGTG CTTGGCGGCA TATTCGTCGG CGCCATTGCT GCCAAGTGTT
     2521 ACAACAAGTT CCACCAAACT CAGCTTCCAA CAGCAATTAG CTTCTTTTCC GGAACCAAGC
     2581 TGGTGCCCAT CATCACATTC GTGGCTGTTA TCCCTTTGTC CTTTATATTT ATGATGGCCT
     2641 GGCCCGTAAT AGGTCTTGGG TTAAACAAAT TTGGTCAGGT TTCTGGCACC CTGCCTTATG
     2701 GTACAGACAG TCTGATATTT GAAATTGTAG AGCGCTCGTT GGTCCCGTTT GGTTTACACC
     2761 ACGTTTTTTA CGCGCCTTTA TGGTGGACTA GTGCAGGGGG TTCTATCGCA GAAGGATTTA
     2821 ATAACCTGAG CAAACAATCT GAAGCCATTC AAAAGGTATT TGTAGACTCG TACAACTCCC
     2881 TTCACGGGAC TCATCACACT AAGCTTCAGG AGATTATCGA TATCGTTCAG AAAAATAAGG
     2941 AATTGTGGGG CGCTGTAGGT GATCAGATTA TCTCTCAGAG AGTCATAGGA CACCTGAATA
     3001 TTTTAAACTT TACCGACGTT GAGAAACTTG GGATAAACCT GGGGCGGTTT CAGTCCGGCA
     3061 AGTTTGGATT TATGCTTTTG GGGCTTCCAG CCGCCGCATT GGCCATGTGG CTGGCCGCAC
     3121 CAAAAGAAAA CCGCCAACAA GTTTTTGGGA TATATTTCTC CGCGGCATTC ACTTGTTTTT
     3181 TGACAGGTAT CACCGAACCA ATAGAGTATA CATTTCTGTT CGTCGCCCCA TGGTTGTTCT
     3241 ATGGAGTACA CATGCCGTTG GCGTCTATCG CTTTCTGGGC TACAGGCGCC CTGCAAACGC
     3301 ATATCACTCA AACAGTCTCA GGGGGTATCA TCGATTACAT CGTGTTTGGC ATTATCCCGT
     3361 TCATTTCGGG TGCAATGAAA CCGATCAGCG CTTTCGGTGT ACTTGGAGTA GCTGTGGTTC
     3421 TTGCTCCTAT CTACTTCTGC GCCTTCTATT TCCTGATCAA GTTATTTAAC GTCAAGACTC
     3481 CTGGTCGGGA TGGCAACGCT GAGGCGAAGC TGTACACGAA AGCCGACTTC AAAGCCTCTA
     3541 AAGGTCTTAA TGTTGACGGT AGTAAGATGT CGTCAAGCAC GGACGACAAA GAACAGGCTA
     3601 GATTGGCGAA GGCAGCCGCC ATTATTGAAT ACTTAGGTGG AGAGGAAAAT ATCGTAGACG
     3661 TAGATTCATG TGCTTCCCGG TTGCGGTTGA CGGTGGTTGA CGCCAAAAAA GCTGATATAG
     3721 ATGGGATTAA AAGCCTTGGT GGAACAACAG GTGCGTTAGT CAAGGGAAAT AATATACAGA
     3781 TAGTGTACGG AGGAGAACAG GAGGCCATCA AGCCACGCAT GCAGAAACTG CTGGAACAGC
     3841 AAAGACAGGA AAAGATGATG GGACACAGTG AGATGAAATC CGAGGAGATG AAGAGTGAGA
     3901 ATATGTCTAT GACATGCGAA AGCAATCAAG CCTGTGATAA AAAGGACGAA GCGTGCGGCT
     3961 GTGAGAAGGA CTGCATGTGT GAGGAACCGA ACAAAGACGA GGAGCAGCCA GTGAGTGAAA
     4021 TGAAAGTTGA GGAAAAAGTA GTCGATGAGA TGAAGGTAGA AAAGCCCAAG CAAACACGGA
     4081 CCAAAAGTAC CAAGGCTAAG TCGACTACTT CTAAGTCGAC TAAGTCGACA TCCAAATCTT
     4141 CGTCCAAGTC CACAAAAAGT ACATCATCGA AAGCAAAATC GACCAGTTCA AAACCGAAAA
     4201 CTACCAAAGC CAAGTCAACG TCATCCAAGA CTAAAACAAC CAAGTGAAGA GCTTAGAGAC
     4261 C
//