LOCUS Exported 4852 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..4852 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 38..85 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 1933..1949 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 1950..1981 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 2010..4838 /label="MMSYN1_0787" /note="" /translation="MFLFKKRKFSPKKITRHKKKTHFANERFIKQVSNLEQNEVLEIMQLQHFGLTNEQYESRLKKYGTNELKKKRFNLIAEFLHAFFGPFNIVLLLISLYNFISYATNGFYQDTNSSDSKFELVGALIILVMVLASGLASFIQSLRSHLVTKKISSIVKSTTNIIRHKNDEDVEDYLKITKRNQLDLIRLGEEIDVKQLVPGDLIYLSSGDMLPADVRIIQSTDLFINQSSLTGESIPVEKHANNKKNTNNILDLENICYTGTSVVSGSALAVVLATANDTYFSTISKAILEKRPDSSFTKGIKQVTRMLLIFMLVMVPTVYLAKSIIGTISSGGSFDSIKDNPWFQAIFFAVAVAVGLTPEMLPMIVTTNLANGASKMSKQKVVVKQLEAIQSLGAIDVLCTDKTGTLTNDKIELVDYLRVDKKADPTLLKYLYINSYYQTGLKNPMDKAIVDYVNKHNHNFSIQDITKIDEIPFDFNRRKLTIIFDDENEKRFMVTKGSVEEILNSCTRVIQDDKVVNLTDTFKRQIIAYYETINQQGKRLLGVAYKKIRDNQAKFSPKDEESLIFMGFASFLDTPKPSTKQTIKLLKKYGVDLKILTGDSEPITRAICKMVNLDIKGLVTGEEIDAASEYELKKIVEDNNIFVKLNPLQKVKIIQVLKQNNHVVGYMGDGINDAPVLRQSDVAISVNNATEIAKDASDIILLEKSLLVLEKGIIQGRTIFGNILKYIKITTASNFGNALSVLIGTVWLPFSPMAPAQILLQNLIYDFSQFGVALDRVDSTFLTSPQRWQSKDLLPFTTINGSISTIFDLITFAIAGYYFGFITSYNSAIAQNNSLLAAQSLAQFHACWFIIGLLSQTFVFQILRTEQLPVIQSRSTWPVYVIGALATIMAFSIVYISQIGSLVQLQSPGLIYIPISIAIIFSYCLIAQLTKVGYKKVFKKWL" primer_bind complement(90..106) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(173..761) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(952..1812) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(1813..1917) /label="bla promoter" /note="ampicillin resistance gene promoter" ORIGIN 1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC 61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT 121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA 181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC 241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG 301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC 361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG 421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC 481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA 541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG 601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA 661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG 721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA 781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG 961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG 1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC 1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC 1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA 1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC 1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG 1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC 1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT 1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG 1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC 1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG 1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT 1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG 1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG 1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT 1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC 1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC 1981 TTGGTTCAGG TGGAGTGGGA GGGTCTCAAA TGTTCCTTTT TAAGAAGCGC AAATTTAGTC 2041 CGAAGAAAAT TACCCGCCAT AAGAAAAAGA CACATTTCGC GAATGAGCGC TTTATTAAGC 2101 AAGTCTCGAA TCTTGAACAA AACGAGGTCT TAGAGATCAT GCAGTTGCAA CATTTCGGCT 2161 TAACAAATGA GCAATACGAG TCTCGTTTGA AGAAGTATGG TACGAATGAG CTTAAAAAGA 2221 AACGCTTCAA CTTAATTGCA GAGTTCCTGC ACGCGTTTTT TGGTCCTTTC AATATCGTGT 2281 TACTGTTAAT TAGCTTATAC AACTTCATCT CCTATGCTAC CAATGGGTTT TATCAGGACA 2341 CAAATTCCAG TGACTCTAAG TTTGAGCTTG TAGGCGCGCT GATTATTCTG GTTATGGTAT 2401 TGGCCTCGGG GTTAGCTAGC TTTATTCAGT CGCTGCGCTC GCATTTAGTG ACTAAGAAAA 2461 TCTCTTCAAT CGTAAAGTCC ACTACTAATA TCATCCGCCA CAAAAATGAC GAGGATGTAG 2521 AGGACTACTT GAAGATCACA AAGCGTAACC AGTTAGACCT GATTCGTCTT GGAGAGGAGA 2581 TCGATGTTAA GCAATTGGTG CCAGGCGACT TGATTTATTT GTCTAGCGGG GACATGCTGC 2641 CCGCGGACGT CCGCATTATC CAATCAACCG ACCTGTTCAT CAACCAGAGC TCGCTTACGG 2701 GAGAATCTAT TCCTGTAGAA AAACATGCTA ACAACAAGAA GAATACAAAT AATATTTTAG 2761 ACCTGGAAAA CATCTGTTAT ACAGGAACTT CCGTCGTAAG TGGTAGTGCC TTGGCGGTTG 2821 TGTTGGCCAC TGCGAATGAT ACCTACTTTA GTACGATTTC AAAGGCCATT TTGGAAAAGC 2881 GTCCGGACTC CTCCTTTACC AAAGGTATTA AACAGGTCAC TCGCATGCTT TTGATCTTTA 2941 TGCTGGTAAT GGTGCCAACG GTCTATCTTG CCAAGTCGAT TATCGGCACA ATTAGTAGTG 3001 GTGGTTCTTT CGACTCAATC AAAGATAACC CATGGTTCCA AGCAATCTTT TTTGCTGTTG 3061 CTGTCGCGGT TGGGTTGACT CCTGAGATGT TACCTATGAT TGTAACTACG AACTTAGCAA 3121 ACGGGGCCTC TAAAATGAGC AAACAAAAGG TAGTCGTCAA ACAACTTGAA GCCATCCAGT 3181 CTTTAGGAGC GATTGATGTT TTATGTACCG ACAAGACTGG TACCCTGACG AACGATAAGA 3241 TCGAATTGGT GGATTACCTT CGTGTCGATA AAAAGGCAGA TCCCACATTA TTAAAGTATT 3301 TGTACATCAA TTCATACTAC CAGACCGGAC TGAAAAACCC GATGGATAAG GCCATCGTCG 3361 ATTACGTAAA TAAGCACAAC CACAATTTTA GTATTCAAGA TATTACAAAG ATTGACGAGA 3421 TCCCATTCGA CTTCAACCGT CGTAAATTAA CGATTATCTT TGACGATGAG AATGAGAAGC 3481 GTTTTATGGT AACCAAAGGT AGCGTAGAGG AGATCTTGAA CTCCTGTACA CGTGTGATCC 3541 AAGACGATAA AGTAGTCAAC TTGACGGACA CATTCAAACG TCAAATTATT GCATACTATG 3601 AGACTATCAA TCAACAAGGT AAGCGCCTTC TGGGCGTCGC GTATAAGAAA ATTCGTGACA 3661 ACCAAGCAAA GTTCTCTCCT AAGGATGAGG AGTCACTGAT CTTCATGGGG TTCGCATCGT 3721 TTTTGGACAC TCCCAAGCCT TCGACAAAGC AAACTATCAA ATTACTGAAG AAGTACGGGG 3781 TTGACTTGAA AATTTTGACA GGGGATTCCG AACCCATCAC TCGCGCCATT TGTAAAATGG 3841 TCAACTTGGA TATTAAAGGG CTTGTTACTG GCGAAGAGAT CGACGCAGCG TCGGAGTATG 3901 AACTGAAGAA AATCGTGGAG GACAATAATA TTTTTGTCAA GCTGAACCCC TTACAGAAGG 3961 TAAAAATTAT CCAAGTACTT AAGCAAAATA ACCACGTTGT AGGGTATATG GGAGATGGCA 4021 TTAATGATGC CCCAGTGTTA CGCCAAAGTG ACGTAGCGAT CAGCGTAAAT AACGCGACAG 4081 AAATCGCGAA GGACGCCTCT GACATCATTT TACTGGAGAA ATCACTGCTT GTACTTGAGA 4141 AAGGTATTAT TCAGGGTCGT ACGATCTTCG GCAATATTTT GAAGTATATC AAAATCACGA 4201 CGGCAAGTAA TTTTGGTAAT GCTTTATCCG TACTGATTGG AACGGTATGG CTTCCATTTA 4261 GTCCGATGGC ACCCGCACAA ATTTTACTTC AAAATTTAAT CTATGACTTC AGTCAATTCG 4321 GTGTAGCTCT TGACCGCGTG GACTCGACCT TCTTGACGAG CCCCCAGCGT TGGCAAAGTA 4381 AGGACTTATT GCCTTTTACT ACTATTAATG GGAGCATTTC TACCATCTTT GATTTGATCA 4441 CTTTCGCGAT TGCGGGGTAT TATTTCGGCT TCATCACATC GTATAATAGT GCCATTGCTC 4501 AGAACAATAG TCTGTTGGCA GCACAGTCGC TTGCACAGTT CCACGCATGT TGGTTTATCA 4561 TCGGACTTCT GTCTCAGACC TTCGTTTTCC AGATCTTGCG TACGGAACAG TTGCCGGTCA 4621 TTCAAAGCCG TTCGACGTGG CCGGTTTACG TAATTGGGGC TCTGGCTACT ATCATGGCGT 4681 TTTCTATTGT TTATATCTCC CAGATCGGAA GTCTGGTGCA ACTGCAATCA CCGGGGTTAA 4741 TTTATATTCC AATCTCTATC GCTATCATCT TCAGTTACTG CCTGATCGCC CAATTGACGA 4801 AAGTTGGATA CAAAAAGGTT TTTAAGAAAT GGCTGTGAAG AGCTTAGAGA CC //