LOCUS       Exported     4852 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4852
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2010..4838
                     /label="MMSYN1_0787"
                     /note=""
                     /translation="MFLFKKRKFSPKKITRHKKKTHFANERFIKQVSNLEQNEVLEIMQLQHFGLTNEQYESRLKKYGTNELKKKRFNLIAEFLHAFFGPFNIVLLLISLYNFISYATNGFYQDTNSSDSKFELVGALIILVMVLASGLASFIQSLRSHLVTKKISSIVKSTTNIIRHKNDEDVEDYLKITKRNQLDLIRLGEEIDVKQLVPGDLIYLSSGDMLPADVRIIQSTDLFINQSSLTGESIPVEKHANNKKNTNNILDLENICYTGTSVVSGSALAVVLATANDTYFSTISKAILEKRPDSSFTKGIKQVTRMLLIFMLVMVPTVYLAKSIIGTISSGGSFDSIKDNPWFQAIFFAVAVAVGLTPEMLPMIVTTNLANGASKMSKQKVVVKQLEAIQSLGAIDVLCTDKTGTLTNDKIELVDYLRVDKKADPTLLKYLYINSYYQTGLKNPMDKAIVDYVNKHNHNFSIQDITKIDEIPFDFNRRKLTIIFDDENEKRFMVTKGSVEEILNSCTRVIQDDKVVNLTDTFKRQIIAYYETINQQGKRLLGVAYKKIRDNQAKFSPKDEESLIFMGFASFLDTPKPSTKQTIKLLKKYGVDLKILTGDSEPITRAICKMVNLDIKGLVTGEEIDAASEYELKKIVEDNNIFVKLNPLQKVKIIQVLKQNNHVVGYMGDGINDAPVLRQSDVAISVNNATEIAKDASDIILLEKSLLVLEKGIIQGRTIFGNILKYIKITTASNFGNALSVLIGTVWLPFSPMAPAQILLQNLIYDFSQFGVALDRVDSTFLTSPQRWQSKDLLPFTTINGSISTIFDLITFAIAGYYFGFITSYNSAIAQNNSLLAAQSLAQFHACWFIIGLLSQTFVFQILRTEQLPVIQSRSTWPVYVIGALATIMAFSIVYISQIGSLVQLQSPGLIYIPISIAIIFSYCLIAQLTKVGYKKVFKKWL"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GGGTCTCAAA TGTTCCTTTT TAAGAAGCGC AAATTTAGTC
     2041 CGAAGAAAAT TACCCGCCAT AAGAAAAAGA CACATTTCGC GAATGAGCGC TTTATTAAGC
     2101 AAGTCTCGAA TCTTGAACAA AACGAGGTCT TAGAGATCAT GCAGTTGCAA CATTTCGGCT
     2161 TAACAAATGA GCAATACGAG TCTCGTTTGA AGAAGTATGG TACGAATGAG CTTAAAAAGA
     2221 AACGCTTCAA CTTAATTGCA GAGTTCCTGC ACGCGTTTTT TGGTCCTTTC AATATCGTGT
     2281 TACTGTTAAT TAGCTTATAC AACTTCATCT CCTATGCTAC CAATGGGTTT TATCAGGACA
     2341 CAAATTCCAG TGACTCTAAG TTTGAGCTTG TAGGCGCGCT GATTATTCTG GTTATGGTAT
     2401 TGGCCTCGGG GTTAGCTAGC TTTATTCAGT CGCTGCGCTC GCATTTAGTG ACTAAGAAAA
     2461 TCTCTTCAAT CGTAAAGTCC ACTACTAATA TCATCCGCCA CAAAAATGAC GAGGATGTAG
     2521 AGGACTACTT GAAGATCACA AAGCGTAACC AGTTAGACCT GATTCGTCTT GGAGAGGAGA
     2581 TCGATGTTAA GCAATTGGTG CCAGGCGACT TGATTTATTT GTCTAGCGGG GACATGCTGC
     2641 CCGCGGACGT CCGCATTATC CAATCAACCG ACCTGTTCAT CAACCAGAGC TCGCTTACGG
     2701 GAGAATCTAT TCCTGTAGAA AAACATGCTA ACAACAAGAA GAATACAAAT AATATTTTAG
     2761 ACCTGGAAAA CATCTGTTAT ACAGGAACTT CCGTCGTAAG TGGTAGTGCC TTGGCGGTTG
     2821 TGTTGGCCAC TGCGAATGAT ACCTACTTTA GTACGATTTC AAAGGCCATT TTGGAAAAGC
     2881 GTCCGGACTC CTCCTTTACC AAAGGTATTA AACAGGTCAC TCGCATGCTT TTGATCTTTA
     2941 TGCTGGTAAT GGTGCCAACG GTCTATCTTG CCAAGTCGAT TATCGGCACA ATTAGTAGTG
     3001 GTGGTTCTTT CGACTCAATC AAAGATAACC CATGGTTCCA AGCAATCTTT TTTGCTGTTG
     3061 CTGTCGCGGT TGGGTTGACT CCTGAGATGT TACCTATGAT TGTAACTACG AACTTAGCAA
     3121 ACGGGGCCTC TAAAATGAGC AAACAAAAGG TAGTCGTCAA ACAACTTGAA GCCATCCAGT
     3181 CTTTAGGAGC GATTGATGTT TTATGTACCG ACAAGACTGG TACCCTGACG AACGATAAGA
     3241 TCGAATTGGT GGATTACCTT CGTGTCGATA AAAAGGCAGA TCCCACATTA TTAAAGTATT
     3301 TGTACATCAA TTCATACTAC CAGACCGGAC TGAAAAACCC GATGGATAAG GCCATCGTCG
     3361 ATTACGTAAA TAAGCACAAC CACAATTTTA GTATTCAAGA TATTACAAAG ATTGACGAGA
     3421 TCCCATTCGA CTTCAACCGT CGTAAATTAA CGATTATCTT TGACGATGAG AATGAGAAGC
     3481 GTTTTATGGT AACCAAAGGT AGCGTAGAGG AGATCTTGAA CTCCTGTACA CGTGTGATCC
     3541 AAGACGATAA AGTAGTCAAC TTGACGGACA CATTCAAACG TCAAATTATT GCATACTATG
     3601 AGACTATCAA TCAACAAGGT AAGCGCCTTC TGGGCGTCGC GTATAAGAAA ATTCGTGACA
     3661 ACCAAGCAAA GTTCTCTCCT AAGGATGAGG AGTCACTGAT CTTCATGGGG TTCGCATCGT
     3721 TTTTGGACAC TCCCAAGCCT TCGACAAAGC AAACTATCAA ATTACTGAAG AAGTACGGGG
     3781 TTGACTTGAA AATTTTGACA GGGGATTCCG AACCCATCAC TCGCGCCATT TGTAAAATGG
     3841 TCAACTTGGA TATTAAAGGG CTTGTTACTG GCGAAGAGAT CGACGCAGCG TCGGAGTATG
     3901 AACTGAAGAA AATCGTGGAG GACAATAATA TTTTTGTCAA GCTGAACCCC TTACAGAAGG
     3961 TAAAAATTAT CCAAGTACTT AAGCAAAATA ACCACGTTGT AGGGTATATG GGAGATGGCA
     4021 TTAATGATGC CCCAGTGTTA CGCCAAAGTG ACGTAGCGAT CAGCGTAAAT AACGCGACAG
     4081 AAATCGCGAA GGACGCCTCT GACATCATTT TACTGGAGAA ATCACTGCTT GTACTTGAGA
     4141 AAGGTATTAT TCAGGGTCGT ACGATCTTCG GCAATATTTT GAAGTATATC AAAATCACGA
     4201 CGGCAAGTAA TTTTGGTAAT GCTTTATCCG TACTGATTGG AACGGTATGG CTTCCATTTA
     4261 GTCCGATGGC ACCCGCACAA ATTTTACTTC AAAATTTAAT CTATGACTTC AGTCAATTCG
     4321 GTGTAGCTCT TGACCGCGTG GACTCGACCT TCTTGACGAG CCCCCAGCGT TGGCAAAGTA
     4381 AGGACTTATT GCCTTTTACT ACTATTAATG GGAGCATTTC TACCATCTTT GATTTGATCA
     4441 CTTTCGCGAT TGCGGGGTAT TATTTCGGCT TCATCACATC GTATAATAGT GCCATTGCTC
     4501 AGAACAATAG TCTGTTGGCA GCACAGTCGC TTGCACAGTT CCACGCATGT TGGTTTATCA
     4561 TCGGACTTCT GTCTCAGACC TTCGTTTTCC AGATCTTGCG TACGGAACAG TTGCCGGTCA
     4621 TTCAAAGCCG TTCGACGTGG CCGGTTTACG TAATTGGGGC TCTGGCTACT ATCATGGCGT
     4681 TTTCTATTGT TTATATCTCC CAGATCGGAA GTCTGGTGCA ACTGCAATCA CCGGGGTTAA
     4741 TTTATATTCC AATCTCTATC GCTATCATCT TCAGTTACTG CCTGATCGCC CAATTGACGA
     4801 AAGTTGGATA CAAAAAGGTT TTTAAGAAAT GGCTGTGAAG AGCTTAGAGA CC
//