LOCUS       Exported     2900 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2900
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      913..960
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2788..2804
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2805..2836
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /note="Produces ATP from ADP in the presence of a proton gradient across the membrane. The gamma chain is believed to be important in regulating ATPase activity and the flow of protons through the CF(0) complex."
                     /translation="MPNLKGLKTEILSVKNISKITNAMQLVASAKLRKISKKVIDTHNYVSEVYSLFNDIISQADKSVFLKDSNFETKKTLWIVINSNLGLCGGYNSNVNKLVLQNLKTNDEIFAIGSKAVSFFKSKKIKIRDQVTNIDINFTNEKAKIISNDLLAMYTNREFDEIKIVYTKFINNVTFEPAIIRIFPIVKSELHFTHKQKIIFEPDADQILNNTISIYINAIIYGTVIESQVSEQASRRTAMENATNNGQNLEHELSLKYNRQRQGAITQEISEIVSGANAQS"
                     /label="MMSYN1_0791"
     primer_bind     complement(965..981)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1048..1636)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1807..2667)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2668..2772)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /label="MMSYN1_0791"
                     /note="Produces ATP from ADP in the presence of a proton gradient across the membrane. The gamma chain is believed to be important in regulating ATPase activity and the flow of protons through the CF(0) complex."
                     /translation="MPNLKGLKTEILSVKNISKITNAMQLVASAKLRKISKKVIDTHNYVSEVYSLFNDIISQADKSVFLKDSNFETKKTLWIVINSNLGLCGGYNSNVNKLVLQNLKTNDEIFAIGSKAVSFFKSKKIKIRDQVTNIDINFTNEKAKIISNDLLAMYTNREFDEIKIVYTKFINNVTFEPAIIRIFPIVKSELHFTHKQKIIFEPDADQILNNTISIYINAIIYGTVIESQVSEQASRRTAMENATNNGQNLEHELSLKYNRQRQGAITQEISEIVSGANAQS"
ORIGIN
        1 ATGCCGAACT TGAAGGGCTT AAAGACGGAG ATCCTTTCAG TGAAGAACAT TTCCAAAATT
       61 ACCAACGCCA TGCAACTGGT GGCGAGTGCT AAATTACGCA AAATTTCCAA GAAAGTAATC
      121 GATACGCATA ACTATGTCTC AGAAGTTTAT TCGCTTTTTA ATGATATCAT CAGCCAGGCT
      181 GATAAATCAG TGTTCTTGAA AGACAGTAAC TTTGAGACAA AAAAGACTCT TTGGATTGTA
      241 ATCAATTCTA ACTTGGGTCT GTGCGGTGGA TATAATAGCA ACGTTAATAA ATTGGTCTTG
      301 CAGAATCTTA AAACAAACGA CGAGATCTTC GCTATCGGGA GCAAAGCAGT CTCCTTCTTT
      361 AAATCCAAGA AGATCAAGAT CCGTGACCAG GTCACCAACA TCGACATTAA TTTTACGAAC
      421 GAGAAGGCAA AGATCATTTC CAACGATCTT CTTGCAATGT ACACGAACCG TGAGTTTGAT
      481 GAGATTAAGA TCGTGTACAC GAAATTTATT AATAACGTCA CTTTTGAGCC TGCTATTATC
      541 CGTATCTTCC CGATTGTTAA ATCTGAATTG CATTTCACAC ACAAGCAAAA GATCATCTTC
      601 GAACCTGACG CGGATCAAAT CCTGAACAAC ACAATCTCTA TCTACATCAA CGCTATTATC
      661 TACGGAACCG TCATCGAATC GCAAGTGTCT GAACAAGCGT CGCGCCGCAC AGCAATGGAG
      721 AATGCGACGA ATAATGGTCA AAACTTAGAG CATGAGTTGA GCTTAAAGTA TAACCGCCAA
      781 CGCCAAGGCG CAATTACCCA AGAAATCAGT GAAATCGTAT CAGGAGCCAA CGCCCAATCC
      841 TGAAGAGCTT AGAGACCGGT ACTAGTTCCT GCAGGGCTCG AGGTGTCAAT CGTCGGAGCC
      901 GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG
      961 CATGGTCATA GCTGTTTCCT GAGAGCTTGG CAGGTGATGA CACACATTAA CAAATTTCGT
     1021 GAGGAGTCTC CAGAAGAATG CCATTAATTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA
     1081 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC
     1141 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA
     1201 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA
     1261 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA
     1321 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA
     1381 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG
     1441 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG
     1501 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG
     1561 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG
     1621 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA
     1681 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT
     1741 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC
     1801 TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC
     1861 ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC
     1921 TGGCCCCAGT GCTGCAATGA TACCGCGAGA ACCACGCTCA CCGGCTCCAG ATTTATCAGC
     1981 AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC
     2041 CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT
     2101 GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC
     2161 TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA
     2221 AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT
     2281 ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG
     2341 CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC
     2401 GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA
     2461 AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT
     2521 GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT
     2581 CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG
     2641 GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA
     2701 TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT
     2761 AGGGGTTCCG CGCACCTGCA CCAGTCAGTA AAACGACGGC CAGTAGTCAA AAGCCTCCGA
     2821 CCGGAGGCTT TTGACTTGGT CAGGTGGAGT GAATTCGCGG CCGCTTCTAG AGGCTCTTCG
     2881 TAATTACGAA GAGGTCTCTA
//