LOCUS Exported 2900 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..2900 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 913..960 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 2788..2804 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 2805..2836 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 1..0 /note="Produces ATP from ADP in the presence of a proton gradient across the membrane. The gamma chain is believed to be important in regulating ATPase activity and the flow of protons through the CF(0) complex." /translation="MPNLKGLKTEILSVKNISKITNAMQLVASAKLRKISKKVIDTHNYVSEVYSLFNDIISQADKSVFLKDSNFETKKTLWIVINSNLGLCGGYNSNVNKLVLQNLKTNDEIFAIGSKAVSFFKSKKIKIRDQVTNIDINFTNEKAKIISNDLLAMYTNREFDEIKIVYTKFINNVTFEPAIIRIFPIVKSELHFTHKQKIIFEPDADQILNNTISIYINAIIYGTVIESQVSEQASRRTAMENATNNGQNLEHELSLKYNRQRQGAITQEISEIVSGANAQS" /label="MMSYN1_0791" primer_bind complement(965..981) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(1048..1636) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(1807..2667) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(2668..2772) /label="bla promoter" /note="ampicillin resistance gene promoter" CDS complement(1..0) /label="MMSYN1_0791" /note="Produces ATP from ADP in the presence of a proton gradient across the membrane. The gamma chain is believed to be important in regulating ATPase activity and the flow of protons through the CF(0) complex." /translation="MPNLKGLKTEILSVKNISKITNAMQLVASAKLRKISKKVIDTHNYVSEVYSLFNDIISQADKSVFLKDSNFETKKTLWIVINSNLGLCGGYNSNVNKLVLQNLKTNDEIFAIGSKAVSFFKSKKIKIRDQVTNIDINFTNEKAKIISNDLLAMYTNREFDEIKIVYTKFINNVTFEPAIIRIFPIVKSELHFTHKQKIIFEPDADQILNNTISIYINAIIYGTVIESQVSEQASRRTAMENATNNGQNLEHELSLKYNRQRQGAITQEISEIVSGANAQS" ORIGIN 1 ATGCCGAACT TGAAGGGCTT AAAGACGGAG ATCCTTTCAG TGAAGAACAT TTCCAAAATT 61 ACCAACGCCA TGCAACTGGT GGCGAGTGCT AAATTACGCA AAATTTCCAA GAAAGTAATC 121 GATACGCATA ACTATGTCTC AGAAGTTTAT TCGCTTTTTA ATGATATCAT CAGCCAGGCT 181 GATAAATCAG TGTTCTTGAA AGACAGTAAC TTTGAGACAA AAAAGACTCT TTGGATTGTA 241 ATCAATTCTA ACTTGGGTCT GTGCGGTGGA TATAATAGCA ACGTTAATAA ATTGGTCTTG 301 CAGAATCTTA AAACAAACGA CGAGATCTTC GCTATCGGGA GCAAAGCAGT CTCCTTCTTT 361 AAATCCAAGA AGATCAAGAT CCGTGACCAG GTCACCAACA TCGACATTAA TTTTACGAAC 421 GAGAAGGCAA AGATCATTTC CAACGATCTT CTTGCAATGT ACACGAACCG TGAGTTTGAT 481 GAGATTAAGA TCGTGTACAC GAAATTTATT AATAACGTCA CTTTTGAGCC TGCTATTATC 541 CGTATCTTCC CGATTGTTAA ATCTGAATTG CATTTCACAC ACAAGCAAAA GATCATCTTC 601 GAACCTGACG CGGATCAAAT CCTGAACAAC ACAATCTCTA TCTACATCAA CGCTATTATC 661 TACGGAACCG TCATCGAATC GCAAGTGTCT GAACAAGCGT CGCGCCGCAC AGCAATGGAG 721 AATGCGACGA ATAATGGTCA AAACTTAGAG CATGAGTTGA GCTTAAAGTA TAACCGCCAA 781 CGCCAAGGCG CAATTACCCA AGAAATCAGT GAAATCGTAT CAGGAGCCAA CGCCCAATCC 841 TGAAGAGCTT AGAGACCGGT ACTAGTTCCT GCAGGGCTCG AGGTGTCAAT CGTCGGAGCC 901 GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG 961 CATGGTCATA GCTGTTTCCT GAGAGCTTGG CAGGTGATGA CACACATTAA CAAATTTCGT 1021 GAGGAGTCTC CAGAAGAATG CCATTAATTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 1081 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 1141 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 1201 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 1261 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 1321 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 1381 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 1441 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 1501 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 1561 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 1621 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 1681 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 1741 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC 1801 TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC 1861 ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC 1921 TGGCCCCAGT GCTGCAATGA TACCGCGAGA ACCACGCTCA CCGGCTCCAG ATTTATCAGC 1981 AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC 2041 CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT 2101 GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC 2161 TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA 2221 AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT 2281 ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG 2341 CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC 2401 GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA 2461 AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT 2521 GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT 2581 CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG 2641 GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA 2701 TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT 2761 AGGGGTTCCG CGCACCTGCA CCAGTCAGTA AAACGACGGC CAGTAGTCAA AAGCCTCCGA 2821 CCGGAGGCTT TTGACTTGGT CAGGTGGAGT GAATTCGCGG CCGCTTCTAG AGGCTCTTCG 2881 TAATTACGAA GAGGTCTCTA //