LOCUS       Exported     3635 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3635
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1648..1695
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3523..3539
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3540..3571
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
                     /label="tonB terminator"
     CDS             1..0
                     /note="Produces ATP from ADP in the presence of a proton gradient across the membrane. The alpha chain is a regulatory subunit."
                     /translation="MSFNIKEISEMIEKQIRNYNKEIVQTEQGTVVSVGDGIALIYGLDNAIMGEFLKFPNNVYGMVLNLEESAVGAVILGDETLIREGDIVKRTNKVVETPVGDALLGRVINALSKPIDNLGPINFTKTKPIERVATSVMARKSVSQPLETGILAIDSAIPIGKGQRELIIGDRQTGKTAIAIDAIINQRNKNVKCIYVAIGQKDSTIVQVVEKLKKYGAMEYTVVVNAGASQPAPLQYLSPYVGITIAEEWMENGSDVLIVYDDLSKHAVSYRQMSLLLRRPPGREAYPGDVFYLHSRLLERAARVNENYGGGSITALPIIETQAGDISAYIPTNVISITDGQIFLSSELFNQGIRPAVDIGPSVSRVGSSAQIKSIKQVSGTLKLELAQYYELESFAKFGSDLDESTKATLDQGAKIIQMLIQKQHNPLEQVDQAILLLTIKSHLIKWLPVESIYNFKHEILSHFKNDKNAFELRKKLDEQKTFDDQLQQQILKEAQKVVLKITKNINEYKPETFGNISEYQNLGK"
                     /label="MMSYN1_0792"
     primer_bind     complement(1700..1716)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1783..2371)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2542..3402)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3403..3507)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /label="MMSYN1_0792"
                     /note="Produces ATP from ADP in the presence of a proton gradient across the membrane. The alpha chain is a regulatory subunit."
                     /translation="MSFNIKEISEMIEKQIRNYNKEIVQTEQGTVVSVGDGIALIYGLDNAIMGEFLKFPNNVYGMVLNLEESAVGAVILGDETLIREGDIVKRTNKVVETPVGDALLGRVINALSKPIDNLGPINFTKTKPIERVATSVMARKSVSQPLETGILAIDSAIPIGKGQRELIIGDRQTGKTAIAIDAIINQRNKNVKCIYVAIGQKDSTIVQVVEKLKKYGAMEYTVVVNAGASQPAPLQYLSPYVGITIAEEWMENGSDVLIVYDDLSKHAVSYRQMSLLLRRPPGREAYPGDVFYLHSRLLERAARVNENYGGGSITALPIIETQAGDISAYIPTNVISITDGQIFLSSELFNQGIRPAVDIGPSVSRVGSSAQIKSIKQVSGTLKLELAQYYELESFAKFGSDLDESTKATLDQGAKIIQMLIQKQHNPLEQVDQAILLLTIKSHLIKWLPVESIYNFKHEILSHFKNDKNAFELRKKLDEQKTFDDQLQQQILKEAQKVVLKITKNINEYKPETFGNISEYQNLGK"
ORIGIN
        1 ATGTCTTTTA ATATCAAGGA AATTTCCGAG ATGATCGAGA AGCAGATCCG TAACTATAAT
       61 AAGGAGATCG TCCAAACGGA GCAAGGTACG GTCGTGTCGG TTGGGGATGG TATCGCTCTT
      121 ATCTATGGCT TGGATAACGC AATTATGGGT GAGTTTCTGA AGTTTCCCAA TAATGTGTAT
      181 GGCATGGTGT TAAACCTGGA AGAATCCGCC GTCGGGGCCG TTATCCTTGG AGATGAAACG
      241 CTTATTCGTG AGGGGGATAT CGTCAAACGT ACCAACAAGG TTGTAGAAAC ACCCGTTGGA
      301 GATGCATTGT TAGGGCGTGT AATCAATGCG TTAAGTAAGC CCATCGACAA CCTGGGACCG
      361 ATTAATTTCA CAAAAACCAA ACCCATCGAG CGCGTAGCTA CCTCGGTTAT GGCACGCAAA
      421 TCGGTGTCTC AGCCTTTGGA GACTGGAATC CTGGCAATTG ATTCTGCCAT CCCAATTGGA
      481 AAAGGGCAGC GCGAATTGAT CATTGGAGAT CGCCAAACCG GTAAGACCGC TATCGCAATT
      541 GATGCAATTA TCAACCAGCG TAATAAAAAT GTTAAGTGCA TTTACGTGGC GATCGGGCAA
      601 AAAGATAGCA CGATCGTTCA AGTCGTGGAG AAACTGAAAA AGTATGGAGC AATGGAGTAC
      661 ACAGTCGTAG TAAATGCCGG TGCAAGTCAA CCCGCCCCAT TGCAGTACTT GAGCCCGTAC
      721 GTAGGGATCA CTATTGCCGA GGAGTGGATG GAGAATGGAA GCGACGTTTT AATTGTTTAT
      781 GACGACCTGT CGAAGCACGC CGTTTCATAT CGCCAAATGT CCCTGCTTCT TCGCCGCCCA
      841 CCAGGGCGCG AAGCGTATCC AGGTGACGTG TTTTATTTGC ATTCCCGTCT TTTAGAGCGC
      901 GCTGCCCGCG TAAACGAGAA CTACGGCGGG GGTTCGATCA CAGCACTGCC TATCATTGAA
      961 ACGCAAGCTG GTGACATCAG CGCGTACATT CCGACGAACG TAATCTCGAT CACGGATGGT
     1021 CAAATCTTCC TTAGTTCGGA ACTTTTTAAT CAGGGCATTC GCCCGGCGGT CGACATTGGT
     1081 CCCTCGGTGT CCCGTGTGGG GTCCTCGGCC CAGATTAAGT CTATCAAACA GGTATCTGGA
     1141 ACTCTGAAGC TTGAGCTGGC TCAATACTAT GAACTTGAAT CTTTCGCCAA GTTCGGGAGT
     1201 GATTTAGACG AAAGTACGAA AGCAACTTTG GATCAAGGCG CAAAGATCAT TCAGATGCTT
     1261 ATTCAAAAAC AACACAACCC CCTTGAACAG GTAGATCAAG CGATTCTGTT GCTTACGATT
     1321 AAGTCGCATT TAATTAAGTG GTTGCCGGTA GAATCTATCT ATAATTTCAA ACACGAAATT
     1381 TTGTCACACT TTAAGAATGA CAAGAATGCA TTCGAGCTTC GTAAGAAGCT TGACGAACAG
     1441 AAAACCTTCG ATGATCAGCT TCAACAACAA ATCTTGAAGG AGGCTCAGAA GGTAGTCTTG
     1501 AAGATCACCA AAAATATTAA TGAGTATAAG CCAGAAACTT TCGGCAACAT CTCAGAATAC
     1561 CAAAACTTAG GGAAGTGAAG AGCTTAGAGA CCGGTACTAG TTCCTGCAGG GCTCGAGGTG
     1621 TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC
     1681 TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT GATGACACAC
     1741 ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA GGCTCCGCCC
     1801 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT
     1861 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT
     1921 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG
     1981 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA
     2041 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA
     2101 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC
     2161 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG
     2221 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG
     2281 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA
     2341 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC
     2401 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG
     2461 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA
     2521 TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT
     2581 CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG
     2641 GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGAACCAC GCTCACCGGC
     2701 TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC
     2761 AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC
     2821 GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC
     2881 GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC
     2941 CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA
     3001 GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT
     3061 GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA
     3121 GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA
     3181 TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG
     3241 GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC
     3301 AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC
     3361 AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA
     3421 TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA
     3481 GAAAAATAAA CAAATAGGGG TTCCGCGCAC CTGCACCAGT CAGTAAAACG ACGGCCAGTA
     3541 GTCAAAAGCC TCCGACCGGA GGCTTTTGAC TTGGTCAGGT GGAGTGAATT CGCGGCCGCT
     3601 TCTAGAGGCT CTTCGTAATT ACGAAGAGGT CTCTA
//