LOCUS       Exported     2681 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2681
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      694..741
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2569..2585
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2586..2617
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /label="MMSYN1_0798"
                     /note="Catalyzes the conversion of uracil and 5-phospho-alpha-D-ribose 1-diphosphate (PRPP) to UMP and diphosphate."
                     /translation="MAFTEIKHPLIIDKLTRMRKTETSSKDFRENLNEIAQLMVYEIFRDLKLEPVEITTPVAKTTGYTINQPVVLVPILRAGIGMLDGIQKLIPTARIAHVGLYRDEETLEIHQYFAKTTKDIDKSYVIVVDPMLATGGSACKAIDIVKQWGAKEVKFVCLVAVEPGIKRLQEQHPDVEIYAASKDEKLNEKGYIVPGLGDAGDRIFGTK"
     primer_bind     complement(746..762)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(829..1417)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1588..2448)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2449..2553)
                     /note="ampicillin resistance gene promoter"
                     /label="bla promoter"
     CDS             complement(1..0)
                     /label="MMSYN1_0798"
                     /note="Catalyzes the conversion of uracil and 5-phospho-alpha-D-ribose 1-diphosphate (PRPP) to UMP and diphosphate."
                     /translation="MAFTEIKHPLIIDKLTRMRKTETSSKDFRENLNEIAQLMVYEIFRDLKLEPVEITTPVAKTTGYTINQPVVLVPILRAGIGMLDGIQKLIPTARIAHVGLYRDEETLEIHQYFAKTTKDIDKSYVIVVDPMLATGGSACKAIDIVKQWGAKEVKFVCLVAVEPGIKRLQEQHPDVEIYAASKDEKLNEKGYIVPGLGDAGDRIFGTK"
ORIGIN
        1 ATGGCGTTCA CCGAAATCAA ACATCCACTG ATCATCGATA AGCTGACACG TATGCGCAAG
       61 ACGGAAACTT CTTCCAAAGA TTTTCGCGAG AATCTTAATG AGATCGCCCA ATTAATGGTC
      121 TACGAAATCT TCCGTGATCT GAAACTTGAA CCAGTTGAAA TCACCACCCC AGTAGCAAAG
      181 ACCACCGGGT ACACGATCAA TCAGCCGGTC GTTTTAGTCC CAATTTTACG TGCTGGGATC
      241 GGTATGTTGG ACGGTATTCA AAAATTGATC CCCACAGCGC GTATTGCCCA TGTGGGTCTT
      301 TATCGTGATG AAGAAACCCT TGAGATTCAC CAGTACTTCG CAAAAACGAC TAAGGATATT
      361 GATAAGTCTT ACGTAATCGT TGTAGATCCC ATGTTGGCAA CCGGCGGAAG TGCCTGTAAA
      421 GCCATTGACA TCGTGAAACA ATGGGGTGCG AAGGAAGTAA AATTCGTATG CCTTGTTGCC
      481 GTAGAGCCTG GAATCAAGCG CCTTCAGGAA CAACATCCCG ATGTAGAAAT CTACGCCGCA
      541 TCTAAGGACG AAAAGTTGAA TGAGAAAGGT TACATCGTAC CAGGCTTAGG GGATGCGGGA
      601 GATCGCATTT TCGGCACTAA GTGAAGAGCT TAGAGACCGG TACTAGTTCC TGCAGGGCTC
      661 GAGGTGTCAA TCGTCGGAGC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA
      721 CGGGTCTTGA GGGGTTTTTT GCATGGTCAT AGCTGTTTCC TGAGAGCTTG GCAGGTGATG
      781 ACACACATTA ACAAATTTCG TGAGGAGTCT CCAGAAGAAT GCCATTAATT TCCATAGGCT
      841 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC
      901 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC
      961 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC
     1021 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG
     1081 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA
     1141 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG
     1201 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA
     1261 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG
     1321 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG
     1381 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC
     1441 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC
     1501 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG
     1561 TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC
     1621 AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC
     1681 GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG AACCACGCTC
     1741 ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG
     1801 TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG
     1861 TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC
     1921 ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC
     1981 ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG
     2041 AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC
     2101 TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG
     2161 AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC
     2221 GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT
     2281 CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG
     2341 ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA
     2401 TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT
     2461 TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG
     2521 TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACCTGC ACCAGTCAGT AAAACGACGG
     2581 CCAGTAGTCA AAAGCCTCCG ACCGGAGGCT TTTGACTTGG TCAGGTGGAG TGAATTCGCG
     2641 GCCGCTTCTA GAGGCTCTTC GTAATTACGA AGAGGTCTCT A
//