LOCUS       Exported     3265 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3265
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2010..3251
                     /label="MMSYN1_0799"
                     /note="Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism."
                     /translation="MNTKINPLIKESLNKELKRQQSHIELIASENYVSQAVLELNGSVLTNKYAEGYPGKRYYGGCEFIDEIESLGIKTAKELFNAEHANIQPHSGSQANDAAYKALLEPKDRVVAMSLDAGGHLTHGYHINFSGNTYDFRFYGVNKDTEQLDYQEIEKIILEHKPKLIVAGASAYSRIIDFKKFREIADKVGAYLMVDMAHIAGLVAAGVHPNPMEYADIVTTTTHKTLRGSRGGLILCKQEFAKKVDSAVFPGSQGGPLENLIAGKTQALLEASTDEFKEYGKQIVKNTKALANVLQENGLRLVAGGSDNHLINVDVKSTLQITGKKAEKILESIGIICNKNMIPFDTEKPFYTSGIRLGTPAMTTRGFKEEEFKQVGLIIVNALKDPSEENLEKLAKQVASLCEKFPIYQSIKY"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GGGTCTCAAA TGAACACTAA GATCAACCCA CTTATTAAAG
     2041 AGTCCTTAAA CAAAGAACTG AAGCGCCAAC AGTCGCACAT TGAATTGATT GCGAGCGAGA
     2101 ATTACGTTAG CCAGGCTGTC TTGGAATTAA ATGGGAGTGT ACTTACGAAT AAATATGCGG
     2161 AGGGCTACCC TGGAAAACGC TATTACGGGG GGTGCGAGTT TATCGATGAG ATTGAGAGTT
     2221 TAGGTATCAA GACTGCGAAG GAGTTGTTCA ATGCTGAGCA TGCTAATATT CAGCCTCATA
     2281 GTGGTTCCCA GGCCAATGAT GCGGCCTATA AGGCATTACT TGAGCCTAAA GACCGTGTCG
     2341 TCGCCATGTC GCTTGACGCT GGAGGTCATT TAACGCACGG ATATCACATC AATTTTTCGG
     2401 GGAATACTTA TGACTTTCGT TTCTATGGTG TTAACAAAGA CACTGAACAA TTGGATTACC
     2461 AAGAGATCGA AAAAATTATC CTTGAGCACA AGCCAAAGTT AATCGTGGCT GGTGCATCTG
     2521 CCTATTCGCG TATCATTGAT TTCAAAAAAT TTCGCGAAAT CGCAGATAAG GTTGGCGCCT
     2581 ATTTGATGGT CGACATGGCA CATATTGCAG GCTTGGTAGC AGCGGGAGTT CACCCAAATC
     2641 CTATGGAGTA CGCAGACATC GTAACAACGA CGACTCATAA AACGTTGCGC GGATCGCGTG
     2701 GAGGGCTTAT CCTTTGCAAA CAGGAGTTCG CGAAGAAGGT GGATTCAGCG GTATTTCCCG
     2761 GCTCTCAGGG AGGTCCATTG GAGAACTTAA TTGCAGGAAA GACGCAGGCT CTGCTGGAAG
     2821 CTAGCACCGA CGAATTTAAG GAGTATGGGA AGCAAATCGT CAAGAATACT AAAGCGTTAG
     2881 CTAACGTGCT GCAAGAAAAC GGGCTTCGTT TGGTAGCAGG AGGCTCCGAC AACCATCTTA
     2941 TCAATGTCGA TGTAAAATCG ACCCTTCAGA TCACTGGGAA GAAAGCAGAA AAGATCCTGG
     3001 AAAGCATCGG TATTATCTGC AATAAAAATA TGATTCCTTT TGACACGGAG AAGCCTTTCT
     3061 ATACCTCCGG TATCCGCCTT GGGACGCCTG CTATGACGAC ACGCGGATTC AAAGAAGAGG
     3121 AATTTAAACA GGTTGGTCTG ATCATTGTGA ATGCCTTGAA AGATCCTAGC GAGGAAAACC
     3181 TGGAAAAACT GGCTAAACAG GTTGCTAGCT TGTGCGAGAA ATTTCCAATT TACCAGTCTA
     3241 TCAAATACTG AAGAGCTTAG AGACC
//