LOCUS       Exported     5791 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..5791
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2010..5777
                     /label="MMSYN1_0803"
                     /note="RpoC: DNA-directed RNA polymerase, beta' subunit"
                     /translation="MENLNRKKAIKIELANPDTIRSWSHGEVLKPETINYKTLKAEKDGLFDERIFGPTKNYECVCGRYKKANPMNKGKKCEKCGVELTESIVRRERMGHIELEEPVTHIWMLKVAPYRIAAILDLKAKELEEVVYFVSHIVLEQGNQKHFIEKEVLDLGSSRITKTREKLQLTILDVIDLINDPNHRDTKKANRLLEELKNTAVPFSIDEATSLISKYTGAKFGIGARAVEYLLEKVDLKKEIEAIKVQLENSKKTPNERTKLLKRLETFDALKRSNQRPEWMVMRVIPVIPPDIRPIIQLDGGRFTTSEINDLYRRIIIRNERLKKVKEMGAPSIIVNNEKRMLQEAVDALFDNERKPKPVQGKNKRPLKSLTSVLKGKQGCFRQNLLGKRVDYSARSVIAIGPDLKMYQAGLPREMAITLFKPFVIQWLQDHEYAENVKIAEKMLLQNDPKVWEALEQVIKDRPVLLNRAPTLHRLGIQAFEPKLVKGKAIRLHPLVTTAFNADFDGDQMAVHVPITKEAVAESRALMLGSSAILGPKDGKAIVTPGQDIILGNYYLTTEEKYAKGQGMIFSSLDEAFMAYKSNQADLNSLIGIALSALPEQKFSDKNQRLNSYLLTTVGKLYFNQIFDDNFPWINSNNIWNAKEAVKEFIYDFSQDIDKVIENVQVQQPIKKKELSLIIERYFETHGARKTAEMLDKMKDLGFSFSTKSGTTISAGDVVAFTHKYDEFKQADQKVEQITDFYNMGMLTNSEKKRRIIEVWSDVKDKIQNELATVLRKDVKNPIFVMVDSGARGNVSNFTQLVGMRGLMNDTKGDIKEIPIKSSFREGLTVSEYFVSTHGARKGMADIALKTADSGYLTRRLVDVSQEIVVVNEDCEPTKGFEVSAIIDTKHDNVIVPLKDRLVGRFTFEDIYDDNKNLIVSANTLIDKNIADKIIMAGISSVIIRSVLTCDNKRGVCQKCYGLNLATASVVNIGEPVGVIAAQSIGEPGTQLTMRNFHTGGVAGNVDITQGLPRIKELLDVTTPKGAVAIISEVDGVVSEIEDYNGVFVINIVTENEEVKKYKTEFNSVLRVEQGSSVVAGQKLTEGAIDLHQLLEFGGIQDVQNYILKEVQKVYRLQGIEISDKYIEIIIKQMLNKVKITDSGDSDLLPGEVITIQNCKEVVQDCIVKSIRPPLSKAQIFGIKKAPLESSSWLSSASFQDTARVLTRAIIKGKEDKLEGLKENIMLGNLIPAGTGLTGTQEVELLAEQYHNNEY"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GGGTCTCAAA TGGAGAATTT GAACCGCAAG AAGGCCATTA
     2041 AGATTGAGTT AGCTAACCCC GATACTATTC GCTCTTGGAG TCACGGCGAG GTATTGAAAC
     2101 CGGAAACCAT TAACTACAAA ACATTGAAGG CTGAAAAAGA CGGACTTTTC GATGAACGCA
     2161 TTTTCGGCCC TACAAAAAAC TATGAGTGTG TCTGCGGTCG TTACAAAAAG GCGAACCCGA
     2221 TGAACAAGGG GAAAAAATGC GAAAAATGCG GCGTTGAATT AACCGAATCG ATCGTCCGTC
     2281 GCGAGCGCAT GGGCCACATC GAACTGGAGG AGCCCGTGAC GCACATTTGG ATGTTAAAGG
     2341 TCGCTCCTTA CCGTATTGCA GCTATCTTAG ACTTAAAAGC CAAGGAACTT GAGGAGGTTG
     2401 TTTATTTTGT CTCGCATATT GTATTGGAGC AGGGGAACCA GAAACATTTC ATCGAAAAGG
     2461 AAGTGTTAGA CTTAGGATCA TCTCGTATTA CGAAAACTCG TGAAAAATTG CAATTAACGA
     2521 TTCTTGATGT GATTGATCTT ATTAACGATC CCAACCACCG TGATACGAAG AAGGCCAATC
     2581 GTTTACTGGA AGAATTGAAA AACACAGCTG TACCCTTTTC TATCGATGAG GCGACGTCGT
     2641 TGATTAGTAA ATACACGGGA GCGAAGTTCG GTATTGGTGC ACGCGCAGTC GAGTACTTGC
     2701 TTGAAAAGGT GGACCTTAAG AAAGAGATTG AGGCAATTAA AGTGCAACTT GAGAATTCCA
     2761 AGAAAACACC GAATGAGCGT ACTAAACTGT TGAAGCGTTT GGAAACCTTC GATGCACTTA
     2821 AACGTTCGAA TCAGCGTCCC GAATGGATGG TAATGCGCGT CATCCCCGTC ATTCCACCCG
     2881 ATATTCGTCC AATTATCCAG TTAGACGGCG GTCGCTTTAC AACTTCGGAG ATTAATGACC
     2941 TTTATCGTCG TATTATCATC CGTAATGAGC GTCTGAAGAA GGTCAAGGAG ATGGGCGCAC
     3001 CTTCAATCAT CGTTAATAAT GAGAAACGTA TGCTGCAAGA GGCTGTCGAT GCGTTATTCG
     3061 ATAACGAACG CAAACCGAAA CCGGTTCAAG GCAAAAACAA ACGTCCACTT AAATCATTGA
     3121 CCTCCGTCTT AAAGGGTAAG CAGGGCTGTT TCCGTCAGAA CTTACTGGGG AAACGCGTGG
     3181 ATTATTCGGC ACGCTCTGTA ATTGCTATTG GGCCGGACCT TAAGATGTAC CAGGCAGGCT
     3241 TGCCCCGCGA AATGGCGATT ACACTGTTCA AGCCATTTGT TATTCAATGG CTGCAGGACC
     3301 ATGAATATGC AGAAAACGTT AAAATCGCGG AAAAGATGCT TTTACAGAAC GACCCAAAAG
     3361 TCTGGGAAGC CTTGGAACAA GTTATCAAGG ATCGTCCCGT ATTGCTGAAC CGTGCCCCAA
     3421 CACTTCATCG TCTGGGAATT CAGGCTTTCG AGCCCAAGCT GGTGAAAGGT AAGGCTATCC
     3481 GTCTGCATCC GTTGGTCACC ACTGCGTTTA ATGCAGACTT CGATGGCGAC CAGATGGCGG
     3541 TGCATGTTCC GATTACTAAA GAGGCAGTTG CAGAAAGTCG CGCTTTGATG CTTGGCAGTT
     3601 CTGCCATTTT GGGGCCGAAA GACGGGAAGG CAATTGTAAC ACCCGGACAA GATATTATTT
     3661 TAGGTAATTA CTACTTAACA ACCGAAGAGA AATACGCGAA GGGTCAAGGC ATGATTTTTT
     3721 CGAGTCTTGA TGAGGCATTC ATGGCATATA AGTCGAATCA AGCGGATTTA AACAGCTTAA
     3781 TTGGTATTGC CTTAAGTGCT CTGCCAGAAC AAAAATTTTC CGACAAGAAC CAACGCCTGA
     3841 ATTCGTATCT GTTGACAACA GTGGGAAAGT TATACTTCAA TCAAATTTTT GATGACAACT
     3901 TCCCATGGAT CAATTCTAAC AACATTTGGA ACGCAAAAGA AGCAGTAAAG GAATTCATTT
     3961 ACGACTTTTC TCAAGACATT GATAAGGTCA TCGAGAACGT ACAGGTGCAG CAACCTATCA
     4021 AGAAGAAAGA ACTTTCCTTA ATCATTGAAC GCTACTTCGA AACGCACGGC GCCCGCAAGA
     4081 CGGCTGAAAT GTTAGATAAG ATGAAGGATC TTGGCTTCTC TTTCTCTACT AAATCCGGAA
     4141 CGACAATCTC CGCCGGGGAT GTCGTTGCCT TCACTCACAA GTACGATGAG TTCAAACAAG
     4201 CCGATCAAAA GGTGGAACAA ATTACTGACT TTTATAACAT GGGCATGCTG ACCAACTCAG
     4261 AGAAGAAGCG CCGCATTATT GAAGTGTGGT CCGATGTGAA AGATAAAATC CAAAATGAAC
     4321 TGGCGACCGT ATTACGTAAA GATGTCAAAA ACCCAATTTT CGTAATGGTT GACTCAGGGG
     4381 CGCGTGGCAA CGTCAGCAAC TTTACCCAAT TAGTGGGTAT GCGCGGGCTT ATGAATGACA
     4441 CCAAGGGAGA CATCAAAGAG ATTCCTATCA AGTCGTCATT CCGCGAGGGC CTTACCGTGA
     4501 GTGAGTATTT CGTATCCACG CACGGTGCTC GTAAAGGGAT GGCTGATATC GCGCTTAAGA
     4561 CGGCAGACAG TGGCTATCTT ACTCGTCGTC TTGTAGACGT CAGCCAGGAG ATTGTGGTCG
     4621 TAAACGAGGA CTGTGAACCT ACTAAAGGCT TCGAGGTTAG TGCTATTATC GACACTAAGC
     4681 ATGACAACGT CATCGTGCCT CTGAAGGATC GTCTTGTGGG GCGTTTTACG TTTGAGGACA
     4741 TCTATGACGA CAATAAAAAT TTAATCGTGT CGGCTAATAC TTTGATTGAC AAGAATATCG
     4801 CGGATAAGAT CATTATGGCT GGTATTAGTA GTGTCATCAT CCGTTCCGTG TTGACTTGTG
     4861 ACAACAAACG TGGCGTCTGC CAAAAATGCT ATGGGCTTAA CTTGGCAACG GCGAGCGTGG
     4921 TTAACATTGG CGAACCAGTT GGAGTAATCG CCGCCCAATC TATTGGGGAG CCGGGTACAC
     4981 AGTTAACCAT GCGCAATTTC CATACGGGGG GTGTGGCCGG AAATGTTGAC ATTACGCAGG
     5041 GTTTGCCACG TATCAAGGAA CTGTTAGACG TAACGACTCC TAAGGGGGCG GTCGCTATCA
     5101 TCAGCGAAGT AGATGGTGTA GTGAGCGAGA TTGAGGACTA CAACGGAGTA TTCGTAATTA
     5161 ACATCGTGAC AGAAAACGAA GAAGTAAAGA AATATAAAAC GGAGTTTAAC TCTGTGTTGC
     5221 GCGTAGAGCA AGGTTCATCA GTCGTAGCTG GTCAGAAGTT GACTGAAGGC GCCATCGATC
     5281 TTCACCAGTT ATTAGAATTT GGTGGTATTC AGGATGTTCA GAACTATATC TTAAAAGAGG
     5341 TCCAAAAAGT ATATCGTTTA CAAGGGATCG AAATTTCCGA TAAGTATATC GAAATTATTA
     5401 TTAAACAGAT GCTTAACAAA GTTAAAATTA CCGACAGCGG CGATAGCGAC TTACTGCCAG
     5461 GAGAAGTCAT CACAATTCAG AACTGCAAAG AGGTTGTTCA GGATTGTATT GTTAAAAGCA
     5521 TCCGCCCTCC CTTAAGTAAG GCACAAATCT TCGGCATTAA GAAGGCTCCT TTAGAGAGTA
     5581 GCAGCTGGCT GTCGTCTGCC TCCTTCCAAG ACACCGCCCG TGTATTGACC CGTGCCATTA
     5641 TTAAAGGTAA GGAGGACAAA TTGGAAGGAC TGAAGGAGAA TATTATGCTG GGCAATTTGA
     5701 TTCCCGCGGG GACAGGTCTT ACGGGAACCC AAGAGGTGGA ATTATTGGCA GAACAATATC
     5761 ACAATAATGA GTACTGAAGA GCTTAGAGAC C
//