LOCUS Exported 3199 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..3199 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 38..85 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 1933..1949 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 1950..1981 /note="bidirectional E. coli tonB-P14 transcription terminator" /label="tonB terminator" CDS 2010..3185 /label="MMSYN1_0805" /note="" /translation="MSFDYFLNNKSLNKINRKLENNIFKTPLPYSLKSKFNYNFIDKISKDRFLSYYTKAFYDDFLESSVEKKLKTYELALLVMNETKIDLDFLSVLKIFRDIKKGKTPTNYLERLIFNIIYAYEYIKKPKVLINEENLEMLISILLVGLEYDLDLKTNYYRTPKTKTLISNVLSSQLISKELENLLDYLKFLQANNLCTYSQTYLIFSTLVLISPFQKYNLIFATLLSQWISFQYNNSYKLVIPICHFLKNQNEYMYELENLLNNDFNADKLINLFNIDYLKNINMYNHASCIYKWVKKDKKRLFIFEDDLSFFVLILILQNTKNLSFNNIKTLLTINKIKLFTDEQIKSTLANLIANQVLQTTSTSVVKYVLVDKYLEKSKYLVNMKGLYNGL" primer_bind complement(90..106) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" rep_origin complement(173..761) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(952..1812) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(1813..1917) /label="bla promoter" /note="ampicillin resistance gene promoter" ORIGIN 1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC 61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT 121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA 181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC 241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG 301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC 361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG 421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC 481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA 541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG 601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA 661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG 721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA 781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG 961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG 1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC 1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC 1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA 1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC 1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG 1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC 1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT 1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG 1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC 1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG 1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT 1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG 1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG 1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT 1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC 1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC 1981 TTGGTTCAGG TGGAGTGGGA GGGTCTCAAA TGTCATTCGA TTATTTTTTA AATAATAAGT 2041 CCCTGAATAA GATCAACCGC AAATTGGAGA ACAATATTTT CAAAACCCCA CTTCCCTATT 2101 CGCTTAAGTC GAAATTCAAC TATAACTTCA TTGATAAAAT CTCTAAGGAC CGCTTTCTGT 2161 CGTATTATAC GAAGGCCTTT TACGATGATT TCTTAGAGAG TAGTGTGGAG AAAAAGCTTA 2221 AAACATATGA ATTGGCGTTA TTGGTGATGA ACGAAACTAA GATCGATCTT GATTTCCTTA 2281 GTGTCCTGAA GATCTTCCGC GACATCAAGA AAGGCAAGAC GCCCACGAAT TACCTTGAGC 2341 GTTTGATCTT CAATATCATC TACGCTTATG AATACATCAA GAAACCTAAG GTCCTGATCA 2401 ATGAGGAGAA CCTTGAAATG CTGATCTCGA TTCTTTTAGT GGGGCTTGAA TATGACCTTG 2461 ACTTAAAGAC GAATTACTAC CGCACCCCAA AGACCAAGAC ACTTATTTCT AACGTACTTT 2521 CTTCCCAATT AATTTCAAAA GAGCTTGAAA ACTTACTTGA TTATTTAAAG TTTCTTCAGG 2581 CAAACAATTT ATGTACTTAT TCACAGACTT ATCTTATTTT CTCGACATTG GTTCTGATTA 2641 GTCCCTTTCA AAAATATAAT TTGATCTTTG CCACGCTGTT GAGCCAATGG ATCTCCTTCC 2701 AGTACAACAA CTCGTATAAA CTTGTAATTC CCATTTGCCA CTTCTTGAAG AACCAGAACG 2761 AATATATGTA CGAATTGGAG AATCTTTTGA ATAATGATTT TAATGCTGAC AAGTTAATCA 2821 ACCTTTTCAA CATCGACTAT TTAAAAAATA TCAATATGTA CAATCACGCG TCATGCATCT 2881 ACAAATGGGT GAAGAAGGAC AAGAAGCGTC TTTTCATTTT CGAAGATGAC TTATCGTTTT 2941 TTGTTTTGAT TCTTATTTTG CAAAATACCA AGAACTTGTC TTTCAATAAT ATTAAGACAC 3001 TTCTGACTAT TAATAAGATT AAGTTATTCA CCGACGAACA AATTAAATCC ACATTAGCGA 3061 ACCTGATTGC TAACCAAGTT TTACAAACTA CTTCGACTTC AGTTGTGAAG TATGTCCTGG 3121 TAGATAAGTA TCTTGAGAAA AGTAAGTACC TTGTAAACAT GAAGGGGCTT TACAACGGGC 3181 TGTGAAGAGC TTAGAGACC //