LOCUS Exported 3056 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..3056 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 1069..1116 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 2944..2960 /note="common sequencing primer, one of multiple similar variants" /label="M13 for" terminator 2961..2992 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 1..0 /label="MMSYN1_0813" /note="GalE: UDP-glucose 4-epimerase" /translation="MNYLLIGGAGYIGSHVAEIINKTDNKVIIYDNLSSGSSDFIEQKSTFIQGDILDFDKLNDVFSSNKIDVVIYLAGLIKVGESVQKPLDYYQTNILGLVNTLKIMQIHNVNYFVFSSSAAVYGNNSRHNGFFYEDDPKEPCSPYGRTKYFGEEIIKDFAIANPNFHYTFLRYFNVAGASKSKRIGYLTQNNNKPTHLIPAISYFAFGLTDKFSIFGSDYNTNDGTCIRDYVYVCELAELHLLTAQKMVKENRNLYYNIGSGKGFSNLEIIKEFERQLGYKLDIDIAQRRSGDPDILVASNTKLCQELNYEIKTNIKDIVESEIAFRKAHLKNK" primer_bind complement(1121..1137) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(1204..1792) /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(1963..2823) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(2824..2928) /label="bla promoter" /note="ampicillin resistance gene promoter" CDS complement(1..0) /label="MMSYN1_0813" /note="GalE: UDP-glucose 4-epimerase" /translation="MNYLLIGGAGYIGSHVAEIINKTDNKVIIYDNLSSGSSDFIEQKSTFIQGDILDFDKLNDVFSSNKIDVVIYLAGLIKVGESVQKPLDYYQTNILGLVNTLKIMQIHNVNYFVFSSSAAVYGNNSRHNGFFYEDDPKEPCSPYGRTKYFGEEIIKDFAIANPNFHYTFLRYFNVAGASKSKRIGYLTQNNNKPTHLIPAISYFAFGLTDKFSIFGSDYNTNDGTCIRDYVYVCELAELHLLTAQKMVKENRNLYYNIGSGKGFSNLEIIKEFERQLGYKLDIDIAQRRSGDPDILVASNTKLCQELNYEIKTNIKDIVESEIAFRKAHLKNK" ORIGIN 1 ATGAATTATT TACTGATCGG GGGAGCAGGC TATATCGGTT CTCACGTCGC TGAAATTATT 61 AACAAGACCG ACAATAAAGT TATCATTTAC GATAACTTGA GTTCAGGCAG TAGTGACTTC 121 ATCGAACAGA AAAGCACCTT TATCCAGGGG GACATTCTTG ATTTTGACAA GTTGAATGAC 181 GTGTTTTCGT CTAATAAAAT CGACGTTGTC ATTTATTTGG CTGGTCTTAT TAAGGTCGGT 241 GAGAGTGTCC AGAAACCGTT AGATTATTAC CAGACAAATA TCTTAGGACT TGTCAATACA 301 TTAAAGATCA TGCAAATTCA TAATGTGAAC TATTTTGTGT TCTCATCTTC AGCTGCAGTA 361 TACGGAAACA ATTCGCGCCA CAATGGTTTC TTCTATGAGG ACGACCCGAA GGAGCCATGT 421 TCTCCTTATG GCCGCACCAA GTATTTCGGA GAAGAAATTA TCAAAGACTT TGCTATTGCA 481 AATCCTAATT TTCATTACAC ATTTTTGCGC TACTTCAACG TGGCGGGTGC ATCAAAAAGC 541 AAGCGCATTG GGTATCTTAC GCAGAATAAC AATAAACCTA CACACTTAAT CCCCGCAATT 601 TCGTATTTCG CCTTCGGATT GACCGACAAG TTTTCCATCT TTGGCTCGGA CTACAATACT 661 AATGACGGAA CGTGCATCCG CGATTATGTC TACGTCTGTG AGCTTGCCGA GCTTCACCTT 721 TTAACGGCTC AGAAAATGGT TAAAGAGAAT CGCAACCTTT ATTACAATAT CGGCAGCGGT 781 AAAGGCTTCT CCAATTTAGA AATCATCAAG GAATTCGAAC GCCAGCTGGG GTACAAATTG 841 GATATTGATA TCGCCCAACG TCGTTCCGGC GACCCAGACA TTTTGGTGGC ATCTAACACT 901 AAGTTGTGTC AGGAATTAAA TTACGAGATC AAGACAAATA TTAAAGATAT CGTGGAGTCG 961 GAGATTGCTT TTCGCAAGGC GCACTTGAAA AATAAGTGAA GAGCTTAGAG ACCGGTACTA 1021 GTTCCTGCAG GGCTCGAGGT GTCAATCGTC GGAGCCGCTG AGCAATAACT AGCATAACCC 1081 CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCATG GTCATAGCTG TTTCCTGAGA 1141 GCTTGGCAGG TGATGACACA CATTAACAAA TTTCGTGAGG AGTCTCCAGA AGAATGCCAT 1201 TAATTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 1261 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 1321 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 1381 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 1441 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 1501 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 1561 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 1621 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 1681 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 1741 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 1801 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 1861 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 1921 TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG 1981 TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT 2041 CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC 2101 GCGAGAACCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC 2161 CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG 2221 GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC 2281 AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG 2341 ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC 2401 TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT 2461 GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC 2521 AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT 2581 ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC 2641 TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC 2701 TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA 2761 AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT 2821 CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG 2881 ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CCTGCACCAG 2941 TCAGTAAAAC GACGGCCAGT AGTCAAAAGC CTCCGACCGG AGGCTTTTGA CTTGGTCAGG 3001 TGGAGTGAAT TCGCGGCCGC TTCTAGAGGC TCTTCGTAAT TACGAAGAGG TCTCTA //