LOCUS       Exported     3245 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3245
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1258..1305
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3133..3149
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3150..3181
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /label="MMSYN1_0814"
                     /note="glf: UDP-GALP_mutase: UDP-galactopyranose mutase"
                     /translation="MNTLKALPTNINSYDYIFIGCGLSTATVCAKLPKDKRILIIEKREHIGGNVYDHKKNDILVHQYGPHIFHTNDKEVFDFLNQFTTFNSYKNVVQAKIDDELIPLPVNVDSIKILFPNEAEDFINYLKEKFPNQEQVTILELSQIDKYQHIYQTIYTRIFASYTGKMWDKKIEDLDVSVFARVPIYLTKRNTYFTDTYEGLPTKGYTQMVLNMLDSSNIDIVLNINITKHLQIKDNQIYINDELITKPVINCAPIDEIFGYKYDKLPYRSLNIKFEELNNPNLQTTAVVNYPEHPKMTRITEYKNFYPEISNDKNTIISKEFPGAFEQNSKEFSERYYPIPNDVSRDQYNKYVEESKKISNLYQLGRLAQYRYINMDQAVRSALDFADELIKKYEK"
     primer_bind     complement(1310..1326)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1393..1981)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2152..3012)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3013..3117)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /label="MMSYN1_0814"
                     /note="glf: UDP-GALP_mutase: UDP-galactopyranose mutase"
                     /translation="MNTLKALPTNINSYDYIFIGCGLSTATVCAKLPKDKRILIIEKREHIGGNVYDHKKNDILVHQYGPHIFHTNDKEVFDFLNQFTTFNSYKNVVQAKIDDELIPLPVNVDSIKILFPNEAEDFINYLKEKFPNQEQVTILELSQIDKYQHIYQTIYTRIFASYTGKMWDKKIEDLDVSVFARVPIYLTKRNTYFTDTYEGLPTKGYTQMVLNMLDSSNIDIVLNINITKHLQIKDNQIYINDELITKPVINCAPIDEIFGYKYDKLPYRSLNIKFEELNNPNLQTTAVVNYPEHPKMTRITEYKNFYPEISNDKNTIISKEFPGAFEQNSKEFSERYYPIPNDVSRDQYNKYVEESKKISNLYQLGRLAQYRYINMDQAVRSALDFADELIKKYEK"
ORIGIN
        1 ATGAACACGT TGAAGGCGCT TCCAACAAAT ATCAATAGCT ATGATTACAT TTTCATCGGT
       61 TGCGGGTTGT CTACCGCTAC GGTGTGCGCG AAATTACCGA AGGACAAGCG CATTCTTATT
      121 ATTGAGAAAC GTGAACACAT CGGGGGAAAC GTTTATGATC ATAAGAAAAA CGACATTTTG
      181 GTACACCAAT ACGGTCCGCA CATTTTTCAT ACCAATGACA AGGAAGTTTT CGACTTCTTG
      241 AACCAGTTTA CCACATTCAA CTCCTATAAG AACGTCGTAC AGGCCAAAAT TGACGATGAG
      301 CTGATCCCAC TGCCCGTCAA TGTTGACAGC ATCAAAATCT TGTTCCCTAA CGAGGCTGAG
      361 GACTTCATCA ATTATTTGAA GGAGAAATTC CCGAACCAAG AGCAAGTCAC AATTCTGGAA
      421 CTGTCCCAAA TTGACAAGTA CCAGCACATT TATCAAACTA TCTACACGCG TATTTTTGCC
      481 TCTTATACGG GGAAGATGTG GGACAAGAAA ATTGAGGATC TTGACGTGTC TGTATTCGCG
      541 CGTGTCCCCA TTTACTTAAC TAAGCGCAAC ACATACTTTA CAGACACCTA CGAGGGGCTT
      601 CCCACAAAAG GATACACCCA GATGGTGCTT AATATGTTAG ACAGTAGCAA CATTGACATC
      661 GTTCTTAATA TCAACATCAC TAAGCATTTA CAGATCAAAG ACAACCAAAT TTATATTAAT
      721 GACGAGTTGA TTACTAAGCC TGTCATTAAC TGTGCCCCCA TCGACGAAAT CTTTGGATAC
      781 AAATACGATA AGTTACCCTA CCGCAGTTTG AATATTAAGT TTGAGGAGTT AAATAACCCT
      841 AATCTGCAGA CAACGGCGGT AGTCAACTAT CCGGAGCACC CAAAAATGAC GCGTATCACA
      901 GAGTATAAGA ATTTCTACCC TGAGATCAGC AATGATAAAA ACACGATTAT CAGTAAAGAA
      961 TTCCCTGGGG CCTTCGAGCA AAACAGTAAG GAATTTTCCG AACGTTATTA TCCAATTCCC
     1021 AATGATGTAT CACGTGACCA ATATAACAAA TATGTCGAAG AGTCCAAGAA AATCAGCAAT
     1081 CTGTACCAGC TTGGGCGCTT AGCTCAGTAT CGCTATATTA ATATGGACCA AGCAGTCCGT
     1141 AGCGCCTTAG ATTTCGCAGA CGAATTAATT AAAAAGTATG AGAAGTGAAG AGCTTAGAGA
     1201 CCGGTACTAG TTCCTGCAGG GCTCGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA
     1261 GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT
     1321 TTCCTGAGAG CTTGGCAGGT GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA
     1381 GAATGCCATT AATTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC
     1441 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG
     1501 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT
     1561 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA
     1621 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC
     1681 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC
     1741 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT
     1801 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT
     1861 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC
     1921 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA
     1981 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
     2041 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
     2101 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
     2161 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     2221 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     2281 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     2341 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     2401 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     2461 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     2521 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     2581 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     2641 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     2701 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     2761 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     2821 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     2881 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     2941 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     3001 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     3061 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     3121 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     3181 TTGGTCAGGT GGAGTGAATT CGCGGCCGCT TCTAGAGGCT CTTCGTAATT ACGAAGAGGT
     3241 CTCTA
//