LOCUS       Exported     3454 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3454
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2010..3440
                     /label="MMSYN1_0818"
                     /note="Catalyzes the transfer of the diacylglyceryl group from phosphatidylglycerol to the sulfhydryl group of the N-terminal cysteine of a prolipoprotein, the first step in the formation of mature lipoproteins."
                     /translation="MKSKLKLKVDKRSIVTTSLWVILSILIISLIVLSSKHFRIDWNQNRGFDTVLEYGGVKDVYLARSDGSGGLRVYALTMTLGMLVAIGFSLYNFNKKDLSISELAIGVIFIIPCSLLGASFFGKLNADGIGRHANGATFWGLFAFWEGGLAIHGGVYVGSIVGLIIFYIIGRKTKVSLLVYADCIIPNILLGQAIGRWGNFFNHEVLGTPVAKIADNISAHTDKAIDQIFSTYLQTHSRPLFLPDFILKNCLSIYSGETQTINNITLENGNVVLLSPIFIYESIALLLGWFVIMFIIPNVWKLFVKPIDKNNNVYKIDYKFSFYQFFNPWLKSTQTKLSSKDAWKKALSDNVYENASEIYINKTSDLDNKKYWEKRVIESHLLNKLNNKNNYPITKAGVQMFAYFLVWNIVRYVLESQRPSDHLFIMHLKNLSLFVIGFSAIIGLIGMIVCQYGLPNISRKPGWLYEVEYFKIRKSN"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
     promoter        complement(1813..1917)
                     /note="ampicillin resistance gene promoter"
                     /label="bla promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GGGTCTCAAA TGAAATCTAA GCTGAAATTA AAGGTCGATA
     2041 AGCGTTCTAT TGTGACTACC TCGCTTTGGG TCATTCTGAG TATCTTAATT ATTAGCCTGA
     2101 TTGTACTTTC TTCGAAGCAC TTTCGTATCG ACTGGAATCA GAACCGCGGA TTCGACACCG
     2161 TATTGGAGTA CGGTGGGGTG AAAGATGTCT ATCTGGCACG CTCAGACGGC AGCGGGGGTT
     2221 TGCGTGTTTA TGCGCTTACT ATGACCCTGG GGATGTTAGT GGCGATTGGT TTCTCATTAT
     2281 ACAATTTTAA CAAGAAGGAT CTGTCTATCT CGGAACTTGC CATTGGTGTG ATTTTCATTA
     2341 TTCCGTGCTC CCTTCTTGGA GCCTCGTTTT TCGGCAAGTT GAATGCTGAC GGTATCGGAC
     2401 GTCACGCGAA TGGGGCCACG TTTTGGGGGC TGTTTGCGTT TTGGGAGGGG GGCCTGGCAA
     2461 TCCACGGGGG AGTGTATGTA GGTAGTATTG TCGGGTTGAT TATTTTCTAC ATTATTGGAC
     2521 GCAAGACAAA AGTAAGTCTT TTGGTTTATG CGGATTGCAT TATCCCTAAT ATTTTGTTGG
     2581 GTCAGGCCAT TGGCCGCTGG GGGAATTTTT TCAACCATGA AGTTCTGGGG ACGCCCGTTG
     2641 CGAAAATCGC CGATAACATT TCAGCACATA CAGACAAGGC CATTGATCAG ATTTTTAGTA
     2701 CCTATCTGCA AACTCACTCG CGTCCATTAT TCTTACCAGA TTTCATTTTA AAAAACTGTC
     2761 TTAGCATTTA CAGTGGCGAA ACACAGACCA TCAACAACAT TACGCTGGAA AACGGGAACG
     2821 TGGTCTTGTT ATCACCAATC TTCATCTATG AGTCGATTGC CCTTCTTTTG GGTTGGTTTG
     2881 TCATTATGTT CATTATTCCG AACGTCTGGA AGTTATTTGT CAAGCCGATT GACAAAAATA
     2941 ACAACGTATA TAAAATCGAC TACAAATTTT CATTCTACCA GTTCTTCAAT CCGTGGCTGA
     3001 AGTCAACCCA GACCAAGCTT TCTTCTAAGG ACGCTTGGAA GAAAGCGTTG AGTGATAATG
     3061 TTTATGAAAA CGCGTCGGAG ATCTACATTA ACAAAACCTC GGATTTGGAT AATAAAAAGT
     3121 ACTGGGAAAA GCGTGTCATC GAAAGCCACC TGTTAAATAA ATTGAATAAC AAAAACAATT
     3181 ATCCGATCAC TAAAGCCGGA GTACAAATGT TCGCTTATTT TCTTGTTTGG AATATTGTTC
     3241 GTTACGTACT TGAGTCCCAG CGTCCATCAG ATCATCTGTT TATCATGCAT TTGAAAAATC
     3301 TTAGTTTGTT TGTGATTGGG TTTAGTGCGA TCATCGGGCT TATTGGTATG ATTGTATGCC
     3361 AGTACGGTCT GCCGAATATC AGCCGTAAAC CGGGTTGGTT GTACGAAGTG GAATACTTCA
     3421 AGATCCGCAA ATCTAACTGA AGAGCTTAGA GACC
//