LOCUS       Exported     2990 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2990
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1003..1050
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2878..2894
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2895..2926
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /note=""
                     /translation="MKNLTNEIYDVIIIGSGPAGLTSAIYTARAGLKTVIYEQSAPGGKLIKTDLIENYPGFETIQGPDLASKMYLQALSLNASVEFVGVNSIFKNADDIFEISLANGETKKAYAVILATGTQENKLGIKGEDELYGKGVSYCAVCDGAFYKNKPVAVVGGGYSAVQEAMYLSQLVDKVYLIVRRDVFRADANKVAKLKAQPNVEFLLKSQVKEIHGTEKVESLTITTPNGEKKLEVSAIFPYIGSTPLIDSVKHLCIENEKGYIPTNDKMQTEIKGLFVAGDVRDVPLRQIAIACGDGAIAGQMAVEYVQEIK"
                     /label="MMSYN1_0819"
     primer_bind     complement(1055..1071)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1138..1726)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1897..2757)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2758..2862)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /label="MMSYN1_0819"
                     /note=""
                     /translation="MKNLTNEIYDVIIIGSGPAGLTSAIYTARAGLKTVIYEQSAPGGKLIKTDLIENYPGFETIQGPDLASKMYLQALSLNASVEFVGVNSIFKNADDIFEISLANGETKKAYAVILATGTQENKLGIKGEDELYGKGVSYCAVCDGAFYKNKPVAVVGGGYSAVQEAMYLSQLVDKVYLIVRRDVFRADANKVAKLKAQPNVEFLLKSQVKEIHGTEKVESLTITTPNGEKKLEVSAIFPYIGSTPLIDSVKHLCIENEKGYIPTNDKMQTEIKGLFVAGDVRDVPLRQIAIACGDGAIAGQMAVEYVQEIK"
ORIGIN
        1 ATGAAGAACT TAACCAATGA GATTTACGAC GTAATTATTA TTGGCTCAGG CCCTGCCGGT
       61 TTAACCAGCG CAATCTACAC AGCCCGTGCC GGCCTGAAAA CCGTTATCTA TGAGCAATCC
      121 GCGCCTGGAG GGAAATTAAT CAAGACAGAC CTTATCGAAA ACTATCCTGG GTTCGAAACG
      181 ATTCAGGGAC CAGACTTGGC GTCGAAGATG TACTTACAAG CCCTGAGCTT GAACGCATCG
      241 GTGGAGTTTG TAGGTGTCAA TAGTATCTTT AAAAATGCCG ATGACATCTT CGAGATTTCG
      301 TTAGCCAACG GGGAAACTAA GAAGGCCTAT GCTGTCATTT TAGCGACCGG GACTCAGGAA
      361 AATAAATTAG GAATCAAAGG GGAGGACGAA CTGTACGGGA AAGGGGTAAG TTACTGTGCT
      421 GTATGTGATG GGGCCTTCTA CAAGAACAAG CCGGTGGCAG TTGTCGGGGG CGGGTACTCC
      481 GCAGTGCAGG AGGCTATGTA CTTGTCACAG TTGGTGGACA AAGTGTACCT GATTGTGCGC
      541 CGTGATGTTT TCCGCGCGGA CGCAAATAAA GTCGCAAAGC TTAAAGCACA ACCTAACGTG
      601 GAGTTTTTAC TTAAATCCCA GGTTAAAGAG ATCCACGGAA CAGAAAAAGT AGAAAGTTTG
      661 ACGATTACAA CGCCAAATGG AGAGAAAAAA CTTGAGGTGA GCGCCATTTT CCCATACATT
      721 GGGTCAACGC CGTTAATCGA TTCCGTCAAG CACTTATGTA TTGAGAATGA AAAAGGCTAT
      781 ATTCCGACGA ATGACAAGAT GCAGACGGAG ATCAAAGGAT TATTCGTAGC TGGGGACGTA
      841 CGTGACGTTC CGTTGCGCCA GATCGCCATT GCGTGTGGTG ATGGCGCAAT TGCAGGCCAA
      901 ATGGCTGTTG AGTATGTCCA AGAAATCAAG TGAAGAGCTT AGAGACCGGT ACTAGTTCCT
      961 GCAGGGCTCG AGGTGTCAAT CGTCGGAGCC GCTGAGCAAT AACTAGCATA ACCCCTTGGG
     1021 GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CATGGTCATA GCTGTTTCCT GAGAGCTTGG
     1081 CAGGTGATGA CACACATTAA CAAATTTCGT GAGGAGTCTC CAGAAGAATG CCATTAATTT
     1141 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG
     1201 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC
     1261 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT
     1321 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA
     1381 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA
     1441 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA
     1501 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA
     1561 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT
     1621 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT
     1681 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT
     1741 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT
     1801 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC
     1861 AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC
     1921 ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA
     1981 GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA
     2041 ACCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG
     2101 CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC
     2161 TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT
     2221 CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG
     2281 GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT
     2341 CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA
     2401 TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA
     2461 GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA
     2521 TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG
     2581 GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC
     2641 ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG
     2701 AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT
     2761 CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT
     2821 ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACCTGCA CCAGTCAGTA
     2881 AAACGACGGC CAGTAGTCAA AAGCCTCCGA CCGGAGGCTT TTGACTTGGT CAGGTGGAGT
     2941 GAATTCGCGG CCGCTTCTAG AGGCTCTTCG TAATTACGAA GAGGTCTCTA
//