LOCUS       Exported     4864 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4864
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2010..4850
                     /label="MMSYN1_0824"
                     /note="UvrA: excinuclease ABC subunit A"
                     /translation="MSTDKIIIKGAREHNLKNIDLELPKNKLIVFTGLSGSGKSSLAFSTIYQEGRRRYIESLSAYARQFLGGNEKPDVDSIEGLSPAISIDQKTTSHNPRSTVGTVTEIYDYLRLLYARIGQPYCINNHGQIKAVSIKEIVENIKQSTSDGEQIHILSPVIRDKKGTHIDILEKLRNDGFIRVIVDDQLRMLDDQINLEKNQRHNIDIVVDRIIYHNNDEINSRIFTAVEMGLKYSNNLIKIAFPNSNKQEKLFSTSFSCKVCDFVVPELEPRLFSFNAPLGACELCNGLGVSLEPDINLILPDLKLSINQGGVVYYKNFMHTKNIEWQKFRILCDYYYIDLNTPLKDLTQKQRDIILWGSDREIDIKIVTENNNKYEKYDFIEGNAALIKRRYFESKSEEARKWYSKFMSSKICKQCKGSRLNDIALSVKINEKSIFDYTNMSISEQLDFLLNIDLTPTQATIAKLVLDEIISRTNFLNEVGLGYLNLSRTATTLSGGESQRIRLAKQIGSQLTGILYVLDEPSIGLHQKDNDKLIKTLKHLRDLGNTLIVVEHDEDTMKSSDWIVDIGPRAGEYGGEITFSGTYQDILKSDTITGRYLSRKEGIVVPKTRRGGNGKKIEIIGASENNLKNINVTIPLNKFITITGVSGSGKSTLLEDIVYKGIHNNLSKEYLPIGKVKEIKGIENINKAIYISQEPIGKTPRSNPATYTSVFDDIRDLFTNLPEAKIRGYKKGRFSFNVSGGRCEHCQGDGVITISMQFMPSVEVVCEICEGKRYNDETLTVKYKNKSIADVLNMSVSEAYVFFENIPQIKQKLETILEVGLGYIKLGQNATTLSGGESQRIKLSTYLLKKQTGNTMFLLDEPTTGLHVDDVKRLIGVLNKLVDLGNTVLCIEHNLDFIKVSDHIIDLGPDGGEYGGQVVVTGTPEQIINHPTSYTAKYLKDYIIND"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GGGTCTCAAA TGTCGACTGA CAAGATCATT ATCAAAGGTG
     2041 CCCGCGAGCA CAATCTGAAG AATATCGATT TAGAACTGCC CAAGAACAAG TTGATTGTAT
     2101 TCACGGGCCT GAGCGGGAGT GGGAAGTCCT CTCTGGCATT TTCGACAATT TACCAGGAGG
     2161 GTCGCCGCCG CTATATCGAA TCTCTGAGCG CTTATGCCCG CCAATTCCTG GGAGGAAATG
     2221 AGAAGCCGGA CGTTGATAGT ATCGAGGGGT TAAGCCCTGC CATTTCAATT GACCAAAAGA
     2281 CTACATCCCA CAACCCTCGT AGCACAGTTG GGACTGTTAC TGAAATCTAT GATTATTTGC
     2341 GTTTGCTGTA TGCACGTATT GGCCAGCCGT ACTGCATTAA TAACCACGGA CAGATCAAAG
     2401 CAGTCTCTAT TAAAGAGATC GTAGAGAACA TTAAGCAATC AACTAGCGAC GGGGAGCAGA
     2461 TTCATATCTT GAGTCCTGTG ATTCGTGACA AAAAGGGAAC ACACATTGAC ATCCTGGAGA
     2521 AGTTACGCAA TGATGGCTTC ATCCGCGTGA TTGTAGACGA CCAATTACGT ATGTTGGACG
     2581 ACCAAATTAA CTTAGAGAAA AATCAGCGCC ATAATATCGA TATTGTAGTG GATCGTATTA
     2641 TCTATCACAA CAATGATGAA ATTAACTCTC GTATCTTTAC GGCTGTTGAA ATGGGTCTTA
     2701 AATATTCAAA CAATTTAATT AAAATTGCGT TTCCAAATTC GAACAAACAA GAGAAGCTGT
     2761 TCAGCACGTC TTTCTCTTGC AAAGTGTGTG ACTTTGTCGT ACCTGAACTT GAGCCCCGCC
     2821 TGTTCTCTTT CAATGCGCCG CTGGGCGCAT GTGAACTGTG CAATGGCCTT GGAGTCAGCC
     2881 TTGAGCCGGA TATTAACCTT ATCCTTCCCG ACTTGAAATT GTCTATCAAC CAAGGCGGTG
     2941 TCGTTTATTA CAAGAACTTC ATGCATACGA AAAACATTGA GTGGCAAAAG TTTCGTATTC
     3001 TGTGCGATTA TTACTATATT GATTTGAATA CACCTTTAAA AGATTTGACG CAGAAGCAGC
     3061 GCGACATCAT TTTGTGGGGT TCGGACCGCG AAATTGACAT CAAAATTGTG ACCGAGAATA
     3121 ACAACAAGTA TGAGAAATAC GATTTTATCG AAGGTAATGC AGCCTTAATC AAGCGTCGCT
     3181 ACTTTGAGTC AAAGTCTGAA GAGGCTCGCA AATGGTACTC CAAGTTCATG TCATCCAAGA
     3241 TCTGCAAGCA ATGTAAGGGT TCACGCCTTA ACGATATCGC CTTGTCCGTC AAAATTAATG
     3301 AGAAATCGAT TTTTGATTAC ACAAATATGA GTATTTCTGA ACAACTTGAT TTTTTACTGA
     3361 ACATCGATCT GACTCCCACT CAGGCGACCA TTGCAAAACT GGTATTGGAC GAAATCATCT
     3421 CCCGCACAAA CTTTCTGAAT GAGGTCGGGT TGGGTTACTT AAATTTGTCT CGCACAGCCA
     3481 CGACTTTGAG TGGGGGCGAA AGTCAGCGCA TCCGTTTGGC AAAACAAATT GGCTCTCAGT
     3541 TAACCGGAAT TTTATACGTC TTGGACGAAC CCAGTATCGG CTTACATCAA AAAGACAACG
     3601 ACAAGTTAAT TAAGACACTT AAACATTTGC GTGACTTAGG TAACACGCTT ATTGTTGTGG
     3661 AACATGATGA GGACACGATG AAAAGCAGTG ATTGGATTGT CGATATTGGG CCTCGCGCCG
     3721 GGGAATACGG GGGAGAGATT ACGTTTAGCG GAACGTACCA AGACATCCTT AAATCTGATA
     3781 CAATTACGGG ACGTTACTTG TCTCGTAAGG AGGGTATCGT AGTTCCTAAG ACCCGTCGTG
     3841 GCGGAAACGG CAAAAAGATT GAGATCATCG GTGCATCTGA AAACAACCTT AAGAACATTA
     3901 ACGTTACGAT CCCTTTAAAT AAGTTTATTA CTATCACTGG CGTTTCGGGT TCGGGAAAGT
     3961 CAACACTTCT GGAAGATATT GTATACAAAG GTATTCACAA TAACTTGTCG AAGGAGTATC
     4021 TGCCGATCGG AAAAGTCAAA GAGATCAAAG GGATCGAAAA CATCAACAAG GCCATTTATA
     4081 TCAGTCAGGA ACCCATCGGG AAAACACCAC GCAGTAACCC CGCTACCTAT ACCTCTGTTT
     4141 TTGACGATAT TCGCGACTTG TTTACAAATC TGCCCGAAGC CAAGATCCGT GGATACAAGA
     4201 AGGGGCGTTT TAGCTTTAAC GTTAGTGGAG GCCGCTGTGA GCATTGCCAA GGCGACGGCG
     4261 TAATCACCAT CTCAATGCAG TTTATGCCGT CGGTAGAAGT AGTCTGCGAG ATCTGCGAGG
     4321 GAAAACGTTA TAATGACGAA ACGCTTACTG TAAAGTACAA GAACAAATCG ATCGCAGACG
     4381 TACTGAACAT GTCGGTTTCC GAGGCCTATG TGTTCTTCGA AAATATCCCA CAAATTAAGC
     4441 AAAAACTGGA AACGATCCTG GAGGTAGGTC TGGGGTATAT TAAGTTAGGC CAGAACGCAA
     4501 CTACGCTGAG TGGAGGTGAG AGTCAGCGTA TCAAACTTTC TACCTATCTG CTTAAAAAGC
     4561 AGACCGGGAA TACCATGTTT TTGCTGGATG AGCCGACAAC TGGTTTACAC GTTGACGACG
     4621 TCAAACGTTT AATCGGCGTG TTAAACAAGT TAGTGGATTT AGGTAACACG GTTTTATGCA
     4681 TTGAACACAA CCTTGATTTT ATTAAAGTCT CCGACCACAT TATTGACTTA GGACCCGACG
     4741 GCGGGGAATA TGGAGGTCAA GTTGTGGTAA CAGGAACCCC TGAGCAGATC ATCAACCATC
     4801 CCACTTCCTA TACCGCTAAA TATCTGAAGG ATTACATTAT TAACGATTGA AGAGCTTAGA
     4861 GACC
//