LOCUS       Exported     4055 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4055
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      2068..2115
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3943..3959
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3960..3991
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /note="UvrB: excinuclease ABC subunit B"
                     /translation="MFIANNKYKLVTKYKPSGDQNQAIEKLNKGIIENKKHQVLLGATGTGKTFTIANIIAKHNKQALVIAHNKTLAMQLYYELKEMFPENRVEYFVSNFDFFQPEAYIPSKDLYIDKDSRQNMELDMMRLSACNALLTRNDTIVVASVAALFALQNPLEYSSAFIELKVGQKIKRNELLTWLVRSGYTRNDIENQLGSFSAKGDVVKIVPGWVNNIMFRISLFDDEIESIHTLNTITNSILDNITTVTIHPAQSYITPQDKLKTICNNIRNELVQRLAELQSENKLLEAQRLEQRTKYDLESLEEFGFCSGIENYSSHLDFRSKGQRPYVLLDYFNNDFITIIDESHITLPQIRGMYNTDRSRKLTLVEYGFRLPSALDNRPLNFDEFNSLIKQVIYTSATPGDYELDLVNHQVVQQIIRPTGLLDPQIEIRKTTNQIDDIINEIHLRKLQNERVFITTLTIRMSEDLTAFLQEKNIKVAYLHSELKTLERSEILNDLRKGVYDVVVGVNLLREGLDLPEVSLVCILDADKQGFLRNYRSLIQTIGRVARNVNGKAIMYADTVSQAMDEAIKETNRRRKIQEEFNKKHNITPKTISKAISESILSEQTKKTLAKAKKIKDKKQKLQTIQQTIDTLRQEMLQAAKELDFERAAILRDTIIELENEKNTN"
                     /label="MMSYN1_0825"
     primer_bind     complement(2120..2136)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(2203..2791)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2962..3822)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3823..3927)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /label="MMSYN1_0825"
                     /note="UvrB: excinuclease ABC subunit B"
                     /translation="MFIANNKYKLVTKYKPSGDQNQAIEKLNKGIIENKKHQVLLGATGTGKTFTIANIIAKHNKQALVIAHNKTLAMQLYYELKEMFPENRVEYFVSNFDFFQPEAYIPSKDLYIDKDSRQNMELDMMRLSACNALLTRNDTIVVASVAALFALQNPLEYSSAFIELKVGQKIKRNELLTWLVRSGYTRNDIENQLGSFSAKGDVVKIVPGWVNNIMFRISLFDDEIESIHTLNTITNSILDNITTVTIHPAQSYITPQDKLKTICNNIRNELVQRLAELQSENKLLEAQRLEQRTKYDLESLEEFGFCSGIENYSSHLDFRSKGQRPYVLLDYFNNDFITIIDESHITLPQIRGMYNTDRSRKLTLVEYGFRLPSALDNRPLNFDEFNSLIKQVIYTSATPGDYELDLVNHQVVQQIIRPTGLLDPQIEIRKTTNQIDDIINEIHLRKLQNERVFITTLTIRMSEDLTAFLQEKNIKVAYLHSELKTLERSEILNDLRKGVYDVVVGVNLLREGLDLPEVSLVCILDADKQGFLRNYRSLIQTIGRVARNVNGKAIMYADTVSQAMDEAIKETNRRRKIQEEFNKKHNITPKTISKAISESILSEQTKKTLAKAKKIKDKKQKLQTIQQTIDTLRQEMLQAAKELDFERAAILRDTIIELENEKNTN"
ORIGIN
        1 ATGTTTATCG CCAATAACAA GTATAAGCTG GTCACGAAAT ATAAACCCTC GGGTGACCAA
       61 AACCAAGCCA TTGAGAAACT TAACAAAGGT ATCATCGAGA ACAAGAAACA CCAAGTGCTT
      121 TTAGGCGCTA CTGGAACTGG CAAGACTTTC ACTATCGCTA ATATTATCGC CAAGCACAAT
      181 AAACAGGCGC TGGTGATCGC ACACAACAAA ACGTTAGCAA TGCAACTGTA CTACGAGTTA
      241 AAGGAAATGT TTCCCGAGAA CCGCGTCGAA TACTTCGTGT CAAACTTCGA TTTCTTTCAA
      301 CCCGAAGCAT ATATTCCGTC AAAAGACCTT TATATCGATA AAGACTCACG CCAAAACATG
      361 GAGCTGGATA TGATGCGTCT GAGCGCGTGT AATGCTCTGC TTACGCGCAA CGATACTATC
      421 GTCGTAGCCT CCGTAGCTGC TCTTTTCGCG TTACAGAACC CGCTTGAGTA TTCTAGCGCT
      481 TTTATTGAAC TGAAGGTTGG GCAGAAAATT AAACGCAACG AGTTGCTTAC TTGGCTGGTT
      541 CGTTCGGGAT ATACCCGTAA CGACATCGAA AACCAATTAG GGTCTTTTTC TGCCAAGGGA
      601 GATGTCGTAA AAATCGTTCC GGGGTGGGTG AACAATATCA TGTTTCGTAT CTCCCTGTTT
      661 GATGACGAGA TCGAAAGTAT CCATACATTA AACACGATTA CTAACAGCAT CTTAGATAAT
      721 ATCACGACGG TAACGATCCA CCCTGCCCAA TCTTACATTA CTCCTCAGGA CAAACTGAAA
      781 ACTATCTGCA ACAATATCCG CAACGAGCTG GTGCAGCGTT TAGCTGAGTT ACAATCGGAG
      841 AATAAACTTC TTGAGGCCCA GCGTCTGGAG CAGCGCACCA AGTATGACTT AGAATCCTTA
      901 GAAGAGTTCG GTTTCTGCTC GGGGATCGAG AATTATTCCA GTCACTTAGA CTTTCGTTCA
      961 AAGGGTCAAC GTCCCTACGT GCTTCTGGAT TATTTTAATA ATGATTTCAT TACTATCATT
     1021 GATGAAAGCC ATATCACATT ACCACAAATC CGTGGCATGT ATAATACTGA TCGTTCACGC
     1081 AAATTAACAT TGGTCGAGTA TGGCTTTCGC CTGCCGTCGG CCTTAGACAA CCGTCCCCTT
     1141 AACTTCGACG AGTTTAACAG TTTAATTAAG CAAGTCATTT ACACATCTGC CACGCCAGGC
     1201 GACTATGAAT TGGACCTTGT AAACCATCAG GTTGTTCAAC AGATCATCCG TCCGACTGGT
     1261 CTGCTGGATC CACAAATTGA GATTCGTAAG ACCACAAACC AAATTGACGA TATTATTAAC
     1321 GAAATTCATT TGCGCAAATT GCAAAACGAA CGCGTTTTCA TTACAACTTT AACTATCCGC
     1381 ATGAGTGAGG ATCTGACTGC CTTTCTGCAA GAGAAAAATA TTAAGGTAGC ATATTTACAC
     1441 TCTGAACTGA AAACTCTTGA ACGTTCTGAG ATTCTTAACG ATTTACGTAA GGGCGTCTAC
     1501 GATGTCGTAG TTGGCGTAAA CTTATTACGC GAGGGCCTGG ATTTACCAGA AGTGTCTTTA
     1561 GTTTGTATTT TAGATGCCGA CAAGCAAGGC TTTTTGCGTA ATTACCGCTC ATTGATTCAA
     1621 ACGATCGGTC GTGTAGCCCG TAACGTAAAT GGTAAGGCCA TTATGTATGC CGACACAGTC
     1681 TCCCAAGCAA TGGATGAAGC CATTAAGGAA ACGAATCGTC GTCGTAAGAT TCAAGAAGAA
     1741 TTCAATAAGA AACACAACAT TACGCCAAAA ACAATCTCTA AGGCGATCAG CGAGTCTATC
     1801 CTTTCAGAAC AAACGAAAAA GACGTTAGCC AAAGCAAAAA AGATTAAGGA CAAAAAGCAA
     1861 AAGCTGCAAA CAATTCAACA GACCATCGAT ACACTGCGTC AAGAAATGTT GCAGGCAGCT
     1921 AAAGAGCTGG ATTTCGAACG TGCTGCAATT CTGCGTGATA CCATCATCGA GTTAGAGAAT
     1981 GAAAAGAATA CCAACTGAAG AGCTTAGAGA CCGGTACTAG TTCCTGCAGG GCTCGAGGTG
     2041 TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC
     2101 TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT GATGACACAC
     2161 ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA GGCTCCGCCC
     2221 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT
     2281 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT
     2341 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG
     2401 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA
     2461 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA
     2521 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC
     2581 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG
     2641 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG
     2701 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA
     2761 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC
     2821 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG
     2881 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA
     2941 TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT
     3001 CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG
     3061 GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGAACCAC GCTCACCGGC
     3121 TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC
     3181 AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC
     3241 GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC
     3301 GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC
     3361 CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA
     3421 GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT
     3481 GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA
     3541 GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA
     3601 TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG
     3661 GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC
     3721 AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC
     3781 AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA
     3841 TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA
     3901 GAAAAATAAA CAAATAGGGG TTCCGCGCAC CTGCACCAGT CAGTAAAACG ACGGCCAGTA
     3961 GTCAAAAGCC TCCGACCGGA GGCTTTTGAC TTGGTCAGGT GGAGTGAATT CGCGGCCGCT
     4021 TCTAGAGGCT CTTCGTAATT ACGAAGAGGT CTCTA
//