LOCUS       Exported     3008 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3008
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
                     /label="T7 terminator"
     terminator      1021..1068
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2896..2912
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2913..2944
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /label="MMSYN1_0826"
                     /note=""
                     /translation="MYFFYSEDIFLLNNQIKKTIKELQQKDQYDVLSFSLIEDDFNTIYDNVTNLNFFSSKSIIVISDAYFVTEIKTNFNKNYSLNKLEIMLKNFNPNNVIIFTLNSNKFSKKLKIAKYIESNFNVKYLSLWDEKQTIKYIIDYLKSKNKIIDINLANQIYNLLPNDLQIITNETNKLANLKSELNRDIIKTNLNKYHNEDIFKLVDAFINNNIDKFIKLYHDYILLNDDIIGLISLLDTNLSFYRDVVILKNQFKSEEQISTILKSHIYRIKLAINNSYDINTLNDKIKIVYKIYKGIINWNINKKTLVEYMLIKNMKG"
     primer_bind     complement(1073..1089)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1156..1744)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1915..2775)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
     promoter        complement(2776..2880)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /note=""
                     /translation="MYFFYSEDIFLLNNQIKKTIKELQQKDQYDVLSFSLIEDDFNTIYDNVTNLNFFSSKSIIVISDAYFVTEIKTNFNKNYSLNKLEIMLKNFNPNNVIIFTLNSNKFSKKLKIAKYIESNFNVKYLSLWDEKQTIKYIIDYLKSKNKIIDINLANQIYNLLPNDLQIITNETNKLANLKSELNRDIIKTNLNKYHNEDIFKLVDAFINNNIDKFIKLYHDYILLNDDIIGLISLLDTNLSFYRDVVILKNQFKSEEQISTILKSHIYRIKLAINNSYDINTLNDKIKIVYKIYKGIINWNINKKTLVEYMLIKNMKG"
                     /label="MMSYN1_0826"
ORIGIN
        1 ATGTATTTTT TCTATAGCGA GGATATTTTC TTGCTTAACA ACCAGATCAA AAAGACCATC
       61 AAGGAATTGC AGCAGAAAGA TCAGTACGAT GTGTTAAGTT TCTCACTGAT TGAGGACGAC
      121 TTCAACACAA TTTATGATAA CGTCACTAAC CTTAATTTTT TCTCTAGCAA GAGTATTATC
      181 GTAATTTCTG ATGCATATTT TGTTACTGAG ATTAAGACGA ACTTTAATAA AAACTATTCA
      241 CTGAATAAGT TGGAAATTAT GCTTAAGAAC TTCAATCCGA ATAATGTTAT TATCTTCACC
      301 CTTAATTCCA ATAAGTTCTC AAAGAAACTG AAGATTGCAA AGTACATTGA ATCGAATTTT
      361 AATGTTAAAT ATCTGAGTCT TTGGGATGAG AAACAGACTA TCAAATATAT CATTGACTAT
      421 CTTAAAAGTA AGAACAAAAT TATTGACATC AACCTGGCTA ACCAGATCTA TAACTTGCTG
      481 CCGAATGACT TGCAAATTAT CACTAACGAG ACTAACAAAT TAGCAAATCT TAAGAGCGAG
      541 CTGAATCGTG ACATCATTAA AACCAATCTT AACAAATACC ACAACGAGGA CATCTTTAAA
      601 TTGGTCGACG CCTTCATCAA TAATAATATC GACAAGTTTA TCAAGTTATA CCATGACTAT
      661 ATCTTGCTGA ACGATGACAT CATTGGCTTA ATCTCCTTAC TTGATACTAA TTTGAGTTTT
      721 TATCGTGATG TGGTGATCTT GAAGAATCAA TTTAAGAGCG AGGAACAGAT TTCAACAATT
      781 CTTAAATCAC ATATCTACCG CATTAAGCTG GCAATCAACA ATTCCTACGA TATTAACACA
      841 CTTAATGACA AAATTAAGAT CGTCTACAAA ATTTATAAGG GCATTATTAA TTGGAACATT
      901 AACAAGAAAA CTCTGGTTGA ATATATGCTG ATCAAAAACA TGAAGGGCTG AAGAGCTTAG
      961 AGACCGGTAC TAGTTCCTGC AGGGCTCGAG GTGTCAATCG TCGGAGCCGC TGAGCAATAA
     1021 CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCA TGGTCATAGC
     1081 TGTTTCCTGA GAGCTTGGCA GGTGATGACA CACATTAACA AATTTCGTGA GGAGTCTCCA
     1141 GAAGAATGCC ATTAATTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG
     1201 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG
     1261 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT
     1321 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT
     1381 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG
     1441 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT
     1501 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT
     1561 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT
     1621 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC
     1681 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC
     1741 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG
     1801 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA
     1861 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA
     1921 ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC
     1981 CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC
     2041 TGCAATGATA CCGCGAGAAC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC
     2101 AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT
     2161 TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT
     2221 TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC
     2281 CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG
     2341 CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT
     2401 TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC
     2461 TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG
     2521 CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT
     2581 TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC
     2641 GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC
     2701 TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA
     2761 ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG
     2821 TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG
     2881 CACCTGCACC AGTCAGTAAA ACGACGGCCA GTAGTCAAAA GCCTCCGACC GGAGGCTTTT
     2941 GACTTGGTCA GGTGGAGTGA ATTCGCGGCC GCTTCTAGAG GCTCTTCGTA ATTACGAAGA
     3001 GGTCTCTA
//